Apyrase type 3

Collagen (Kollagenreagent Horm; Nycomed), convulxin (Enzo Lifesciences) α-thrombin (Roche Diagnostics), adenosine diphosphate (ADP), sodium heparin, human fibrinogen, apyrase type III, prostacyclin (PGI₂), Igepal CA-630 (all from Sigma-Aldrich), U46619 (Alexis Biochemicals) and ECL solution (PerkinElmer) were purchased, collagen-related peptide (CRP) was generated as described [21] (link). Rhodocytin was a generous gift from Prof. Dr. J. Eble (Münster University Hospital, Germany). The anesthetic drugs medetomidine (Pfizer), midazolam (Roche Pharma AG), and fentanyl (Janssen-Cilag GmbH) and the antagonists atipamezol (Pfizer), flumazenil, and naloxon (both from Delta Select GmbH) were used according to the regulation of the local authorities. The antibody against the activated form of integrin αIIbβ3 (JON/A-PE) was from Emfret Analytics. Anti-murine CD84 monoclonal antibody JER1 [19] (link) and other antibodies were generated and modified in our laboratories as described [22] (link).

Midazolam (Roche Pharma AG), fentanyl (Janssen-Cilag GmbH) and the antagonists atipamezol (Pfizer), flumazenil and naloxone (both from Delta Select GmbH) were used according to the regulation of the local authorities. Thrombin (Roche Diagnostics), ADP, low-molecular-weight heparin and human fibrinogen (Sigma-Aldrich), U46619 (Enzo Life Sciences), collagen (Kollagenreagent Horm; Nycomed), apyrase Type III (Sigma-Aldrich), Fura-2 AM and Pluronic F-127 (Molecular Probes) were purchased, collagen-related peptide (CRP) was generated as described [29] (link). Antibodies against Rac1 (Millipore), actin (Sigma-Aldrich), tubulin (Millipore), mDia1 (Abcam), PAK1/2/3, phosphor-PAK1/2 (423/402), Syk, phosphor-Syk (Y519/520), cofilin, phospho-cofilin (Ser3) as well goat anti-rabbit IgG-HRP (all from Cell Signaling) were purchased. The antibody against the activated form of integrin αIIbβ3 (JON/A-PE) was from Emfret Analytics (Eibelstadt, Germany). Annexin-V was generously provided by Jonathan F Tait, University of Washington Medical Center and conjugated to DyLight 488 by standard methods. All other antibodies were generated and modified in our laboratories as previously described [30] (link), [31] (link).