Glycerol quantification kit

Manufactured by Merck Group

The Glycerol Quantification Kit is a laboratory tool designed to accurately measure the concentration of glycerol in various samples. It provides a reliable and efficient method for quantifying glycerol levels, which is essential in various research and industrial applications.

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Lab products found in correlation

Isoproterenol, by Merck Group (1 mentions) Fluostar omega spectrophotometer, by BMG Labtech (1 mentions) Tissuelyser, by Qiagen (1 mentions)

2 protocols using glycerol quantification kit

Quantification of Cellular Glycerol Release

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After treatment of differentiated OP9 cells with various concentrations of CFE and CO, free glycerol content in cell supernatants was quantified using a glycerol quantification kit according to the manufacturer's instructions (Sigma Chemicals). Glycerol was quantified at OD540 with a model Emax (Molecular Devices). Isoproterenol (Merck, Darmstadt, Germany), a known stimulator of lipolysis, was used as a positive control.

Kim M.S., Kim H.R., So H.S., Lee Y.R., Moon H.C., Ryu D.G., Yang S.H., Lee G.S., Song J.H, & Kwon K.B. (2014). Crotonis Fructus and Its Constituent, Croton Oil, Stimulate Lipolysis in OP9 Adipocytes. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 780385.

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Quantification of FFA and Glycerol in Skeletal Muscle

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For FFA and free glycerol determination, 40 mg of Skm tissue were homogenized in 1 ml of 2-isopropanol in a TissueLyser (Qiagen). Five microliters of Skm extracts were used for TAGs quantification using the Glycerol Quantification Kit (Sigma-Aldrich) and a FLUOstar-Omega spectrophotometer (BMG Labtech). All results were adjusted for exact protein content.

Sánchez-González C., Herrero Martín J.C., Salegi Ansa B., Núñez de Arenas C., Stančič B., Pereira M.P., Contreras L., Cuezva J.M, & Formentini L. (2022). Chronic inhibition of the mitochondrial ATP synthase in skeletal muscle triggers sarcoplasmic reticulum distress and tubular aggregates. Cell Death & Disease, 13(6), 561.

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