Microplate vortex mixers

ArrayJet^® Printer Marathon v1.4, JetSpyder™ 12 samples, JetStar™ (ArrayJet, Roslin, UK); Scanner SensoSpot Fluorescence (Miltenyi Imaging GmbH, Radolfzell, Germany); Orbital shaker (FALC Instruments S.r.l.; Treviglio, Italy); Fisherbrand™ Microplate Vortex Mixers (Fisherbrand™, EEUU); T100 Thermal Cycler (Biorad, Hercules/CA, USA); Magnetic 96-Well Separator, Digital Dry Block Heater (Thermo Scientific, Rockford/IL, USA); GenePix^® Pro Microarray Analysis Software (Molecular Devices, San Jose/CA, USA); R statistics software (R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/); MAGPIX^® System of xMAP^® instruments and xPONENT^® Software (Luminex Corporation, Austin, Texas, USA).

Kit ProcartaPlex Human Coronavirus Ig Total Panel 11-plex was used for antigen study as previously reported using xMAP’s methodology (16 (link)). All plasma samples were incubated in Corning^® 96 Well Solid Polystyrene Microplate together with the standard samples for relative quantification, medium and low control CoV and Assay Buffer as blank. Standard serial dilution was made in PCR 8-Tube Strip. All soluble immunoglobulins (S1 protein for all CoV and for SARS-CoV-2 in addition to the spike trimer -S1 + S2-, RBD and N proteins) are captured with a Bead mix. All washes were carried out with Wash Buffer diluted in deionized water and used a Magnetic 96-Well Separator. Detection antibody was made with Ig total Det Antibody diluted with Detection Ab Diluent (1×). Acquisition was made in Reading Buffer with xMAP^® instruments and the MAGPIX^® System software was used for analysis. All incubation steps were made in agitation with Fisherbrand™ Microplate Vortex Mixers and cover the plate with Plate Cover and Black Microplate Lid.