Drp1 sirna

The YFP-fused mito-tracker plasmid (pmito-YFP) was previously described [28] (link). 3-nitropropionic acid (3-NP) and N-acetylcysteine (NAC) were purchased from Sigma (St. Louis, MO USA). Mdivi-1 (3-(2,4-Dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1H)-quinazolinone) was purchased from Enzo life sciences (Farmingdale, NY, USA). A mitoTracker probe was purchased from Invitrogen (Carlsbad, CA). The validated siRNA targeting for Cpn10 (#1, 5′-CAAAGUAGUUCUAGAUGAC-3′), (#2, 5′-GCGUGAAAGUUGGAGAUAA-3′) negative scrambled siRNA (5′-CCUACGCCACCAAUUUCGU-3′) were purchased form Dharmacon (Thermo Scientific). And previously validated Drp1 siRNA (5′-GAGGUUAUUGAACGACUCA-3′) and Opa1 siRNA (5′-CUGGAAAGACUAGUGUGUU-3′) were synthesized from Bioneer (Daejeon, Korea).

YFP-fused mito-tracker plasmid (pmito-YFP) was previously described [32] . GFP-fused hnRNP A1 plasmid (pEGFP-hnRNP A1) was purchased from Origene Technologies (Rockville, MD). 3-nitropropionic acid (3-NP) was purchased from Sigma (St. Louis, MO). A mitoTracker® probe and Hoechst 33,342 dye were purchased from Invitrogen (Carlsbad, CA). The validated siRNAs targeting for hnRNP A1 (#1, 5′-GCUCUUCAUUGGAGGGUUG-3′), (#2, 5′-CUACAAUGAUUUUGGGAAUUU-3′) and a negative scrambled siRNA (5′-CCUACGCCACCAAUUUCGU-3′) were purchased from Dharmacon (Thermo Scientific). Previously validated Drp1 siRNA (5′-GAGGUUAUUGAACGACUCA-3′) was synthesized from Bioneer (Daejeon, Korea) [32] .