Poly(I:C) (HMW) VacciGrade was purchased from Invivogen (Toulouse, France). Control RNA (5-GCG CUA UCC AGC UUA CGU ATT-3), siRNA against human MDA5 (5GUAUCGUGUUAUUGGAUUATT-3) and TLR3 (5GGUUGGUAAGGAUUCCUUU GCTT-3) were designed according to published guidelines and purchased from Eurofins MWG Operon (Ebersberg, Germany). In vitro transfection of cell lines with RNA was performed using Lipofectamine RNAiMax (Invitrogen, Darmstadt, Germany). For in vivo administration, poly(I:C) was complexed with in vivo-jetPEI (Peqlab, Erlangen, Germany) at an N/P ratio of 6 in 5% glucose solution. IFNα and zVAD-fmk were from Merck Millipore (Darmstadt, Germany), ELISA for CXCL10 from R&D Systems (Wiesbaden, Germany) and for IFNα from PBL Interferon source (Lörrach, Germany). The peptide p15E604611 was synthesized by Jerini Peptide Technologies (Berlin, Germany).
Elisa for cxcl10
Manufactured by R&D Systems
Sourced in Germany
The ELISA for CXCL10 is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of CXCL10 levels in biological samples. The assay utilizes a specific antibody coated on a microplate to capture CXCL10 from the sample, and a second antibody conjugated to an enzyme for detection. The enzymatic activity is proportional to the concentration of CXCL10 present in the sample.
Automatically generated - may contain errors
2 protocols using elisa for cxcl10
1
Poly(I:C) Immune Activation Protocol
2
Quantification of Chlamydia Infection and Immune Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
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C. trachomatis LGV-L2 RifR pGFP::SW2, WT C. trachomatis LGV-L2, RST5 LGV-L2, RST17 LGV-L2, or Targetron-mediated CPAF mutant LGV-L2 was grown and purified as previously described49 (link),51 (link),63 (link). Each preparation was titered uniformly by counting inclusion forming units on monolayers of Vero cells. C. trachomatis was diluted in RPMI with 10% FBS and added at MOI 5 in 100 µl in 96-well plates, mixed via pipetting, and centrifuged onto cells at 1500×g for 30 min at 4 °C. At 27, 46, and 70 h, cells were mixed and 25 µl was used for flow cytometry quantification of C. trachomatis burden and cell death. At 70 h, 25 µl of cell culture supernatant was measured by custom Luminex assay for 17 human cytokines (Millipore), or 50 µl of cell culture supernatant was measured by ELISA for [CXCL10] or [RANTES] (R&D).
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