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Urobilinogen

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Urobilinogen is a laboratory product that is used to measure the level of urobilinogen in biological samples, such as urine or blood. Urobilinogen is a metabolic byproduct formed during the breakdown of hemoglobin in the body.

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Lab products found in correlation

Biliverdin, by Merck Group (2 mentions) Conjugated bilirubin, by Frontier Scientific (2 mentions) Unconjugated bilirubin, by Merck Group (2 mentions) Synergymx multi mode microplate reader, by Agilent Technologies (2 mentions)

2 protocols using urobilinogen

1

Bilirubin Pathway Metabolites Modulate Trypsin Activity

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Metabolites within the bilirubin pathway were tested for the ability to inhibit trypsin activity in vitro. Biliverdin (Sigma Aldrich, 30891), mesobilirubin (Frontier Specialty Chemical, M588-9), urobilinogen (Mybiosource, MBS173206, unconjugated bilirubin (Sigma Aldrich, B4126) and conjugated bilirubin (Frontier Scientific, B850) were reconstituted to 1mg/mL. These stock solutions were then used to prepare a series of dilutions. 69μL of buffer (0.046M Tris-HCl, 0.0115M CaCl2, pH 8.1) was added to a black bottom plate along with 3μL of trypsin (10ng/μL) and 3μL of either unconjugated or conjugated bilirubin. A 100μM substrate solution of N-p-Tosyl-Gly-Pro-Arg 7-amido-methylcoumarin HCl was prepared in buffer as previously described and kept on ice. 75μL of substrate was automatically dispensed into each well to give a final reaction volume of 150μL. Reactions were briefly shaken and then transferred to a Synergy Mx Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA) and read kinetically every 30 seconds for a total of 5 min at 37°C. ΔRFU/min were calculated in the linear range and trypsin activity was expressed as a ratio of the fluorescence measured in the control trypsin well to the fluorescence measured in the presence of the potential metabolite inhibitor.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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2

Bilirubin Pathway Metabolites Modulate Trypsin Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metabolites within the bilirubin pathway were tested for the ability to inhibit trypsin activity in vitro. Biliverdin (Sigma Aldrich, 30891), mesobilirubin (Frontier Specialty Chemical, M588-9), urobilinogen (Mybiosource, MBS173206, unconjugated bilirubin (Sigma Aldrich, B4126) and conjugated bilirubin (Frontier Scientific, B850) were reconstituted to 1mg/mL. These stock solutions were then used to prepare a series of dilutions. 69μL of buffer (0.046M Tris-HCl, 0.0115M CaCl2, pH 8.1) was added to a black bottom plate along with 3μL of trypsin (10ng/μL) and 3μL of either unconjugated or conjugated bilirubin. A 100μM substrate solution of N-p-Tosyl-Gly-Pro-Arg 7-amido-methylcoumarin HCl was prepared in buffer as previously described and kept on ice. 75μL of substrate was automatically dispensed into each well to give a final reaction volume of 150μL. Reactions were briefly shaken and then transferred to a Synergy Mx Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA) and read kinetically every 30 seconds for a total of 5 min at 37°C. ΔRFU/min were calculated in the linear range and trypsin activity was expressed as a ratio of the fluorescence measured in the control trypsin well to the fluorescence measured in the presence of the potential metabolite inhibitor.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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