SOG was bought from Shanghai Yuanye Bio-Technology (Shanghai, China). RPMI 1640 Medium, TRIzol reagent, fetal bovine serum (FBS), streptomycin and penicillin were from Invitrogen-Gibco (Grand Island, NY, U.S.A.). LPS, dimethyl sulfoxide (DMSO) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetra-zolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.). Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 and mouse TNF-α were obtained from eBioscience (San Diego, CA, U.S.A.). The antibodies against phosphorylated proteins including p65, ERK1/2, p38, JNK and β-actin were obtained from Cell Signaling Technology (Danvers, MA, U.S.A.). Other antibodies applied in the present study were from Bioworld (St. Louis Park, MN, U.S.A.).
Enzyme linked immunosorbent assay elisa kits for il 6
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 are laboratory test kits designed to detect and quantify the levels of the interleukin-6 (IL-6) protein in biological samples. These kits utilize antibody-based detection methods to measure the concentration of IL-6 present in the samples.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Mouse tnf α, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Sodium thioglycolate, by Merck Group (1 mentions)
Radioimmunoprecipitation assay ripa buffer, by Abcam (1 mentions)
Pierce enhanced chemiluminescence ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Rpmi medium, by Merck Group (1 mentions)
Lps from escherichia coli o111 b4, by Merck Group (1 mentions)
2 protocols using enzyme linked immunosorbent assay elisa kits for il 6
1
Modulation of Inflammatory Responses
2
Inflammatory Response Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TNBS, sodium thioglycolate, LPS from Escherichia coli O111:B4, and Roswell Park Memorial Institute (RPMI) medium were obtained from Sigma-Aldrich (USA). Radio immunoprecipitation assay (RIPA) buffer was obtained from Abcam (UK). Enzyme-linked immunosorbent assay (ELISA) kits for IL-6, IL-1β, and TNF-α were purchased from eBioscience (USA). Antibodies against p-p65, p65, cyclooxygenase (COX)-2, inducible NO synthase (iNOS), and β-actin were procured from Cell Signaling Technology (USA) and Abcam (USA). A Pierce enhanced chemiluminescence (ECL) western blotting substrate was obtained from Thermo Fisher Scientific (USA). The Gram staining kit was obtained from BioMerieux (France).
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