Elav geneswitch

Flies were reared at 25 °C in medium containing Nutri-Fly^TM Bloomington formulation (Genesee Scientific, San Diego, CA), Jazz mix (Fisher Scientific, Waltham, MA, USA), sugar and powdered yeast (Genesee Scientific) in an 8:5:1:1 ratio and made according to standard procedures. The following fly stocks were used in this study: UAS-Par-1, UAS-Par-1^T408A, and UAS-Par-1^RNAi (All gifts from Bingwei Lu, Stanford School of Medicine, Stanford, CA, USA^{25 (link)}), slmb^3A1 (Bloomington Stock Center)^{52 (link)}, olk^{1 (link)} and olk^{3 (link)} (a gift from Doris Kretzschmar, Oregon Health and Science University, Portland, OR, USA)^{53 (link)}. The following GAL4 lines were used: BG380-Gal4 (A gift from Aaron DiAntonio, Washington University Medical School, St. Louis, MO, USA)^{26 (link)}, and ELAV-GeneSwitch (Bloomington Stock Center)^{27 (link)}.

w¹¹¹⁸, y'w’, OreR, ry⁵⁰⁶, elav::GeneSwitch, and elav::Gal4^c155 were obtained from the Bloomington Drosophila Stock Center. UAS::G6PD was generously provided by Dr. William C. Orr. Puc^E69 was a gift from Dr. Dirk Bohmann.
Female flies were used throughout. Flies were reared on yeast/molasses‐based food (agar [13.8 g], molasses [22 g], malt extract [75 g], dry yeast [Inactivated, 18 g], corn flour [80 g], soy flour [10 g], propionic acid [6.2 ml], methyl 4‐hydroxybenzoate [2 g], EtOH [7.2 ml], H₂O [1 L]) at 25°C, and 65% humidity on a 12‐hr light/dark cycle.

Elav-GeneSwitch, UAS-hTau^0N3R, UAS-Atg1 were obtained from Bloomington Drosophila Stock Center, Indiana, US. Bigenic flies (UAS-hTau^0N3R/UAS-Atg1) were created using standard genetic recombination methods. The flies were raised and maintained at 25°C on SYA diet using standard protocols.