Egfp rab7a

Manufactured by Addgene

Sourced in China

EGFP-Rab7A is a plasmid that expresses the late endosome marker Rab7A fused to enhanced green fluorescent protein (EGFP). Rab7A is a small GTPase that regulates late endosome and lysosome dynamics.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (4 mentions) Lamp1 gfp, by Addgene (2 mentions) Mruby clathrin, by Addgene (2 mentions) Pegfp sec23a, by Addgene (2 mentions) Zsgreen rab5, by Addgene (2 mentions) Psnapf tomm20, by Thermo Fisher Scientific (2 mentions) Psnapf cox8a, by Addgene (2 mentions) Nunc glass bottom dishes, by Thermo Fisher Scientific (2 mentions) Emtb 3xgfp, by Addgene (2 mentions) Pegfp parkin c431s, by Addgene (1 mentions) Halo tomm20 n 10, by Addgene (1 mentions) Lamp1 rfp, by Addgene (1 mentions) Pegfp parkin wt, by Addgene (1 mentions) Pmturquoise2 n1, by Addgene (1 mentions) Lamp1 mgfp, by Addgene (1 mentions) Mapple tomm20 n 10, by Addgene (1 mentions) Pmxs 3xha egfp omp25, by Addgene (1 mentions) Pgex 4t 3 mr7bd, by Addgene (1 mentions) Pspax2, by Addgene (1 mentions)

3 protocols using egfp rab7a

Fluorescent Protein Transfection Workflow

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Cells were grown overnight in 24-well plates at 37°C in a 5% CO₂ atmosphere. After reaching over 80% confluence, the plasmids mRuby-Clathrin (Addgene #55852), pEGFP-Sec23A (Addgene #66609), ZsGreen-Rab5 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.), EGFP-Rab7A (Addgene #28047), GFP-LAMP1 (Addgene #16290), EMTB-3XGFP (Addgene #26741), pSNAPf-Cox8A (Addgene #101129), or pSNAPf-TOMM20 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.) was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. After 24 h, the transfected cells were digested with trypsin-EDTA and seeded into Nunc Glass Bottom Dishes (Φ 12 mm, Thermo Fisher Scientific, Inc.) at a density of 1.5 ~ 2.0 × 10⁴ per well in growth medium (150 μL). The cells were grown for an additional 12 ~ 24 h before incubation with the indicated probes.

Han Y., Zhang Z., Liu W., Yao Y., Xu Y., Liu X., Kuang C, & Hao X. (2021). A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging. Frontiers in Chemistry, 8, 601436.

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Plasmid Sourcing and Cloning for Organelle Imaging

Cited 2 times

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The following plasmids were obtained from Addgene: mApple-TOMM20-N-10 (Addgene; 54955), mCitrine-N1 (Addgene; 54594) (27 (link)), and EBFP2-Lysosomes-20 (Addgene; 55246) were gifts from M. Davidson; LAMP1-mGFP (Addgene; 34831) (28 (link)) was a gift from E. Dell-Angelica; pmTurquoise2-N1 (Addgene; 60561) (29 (link)) was a gift from D. Gadella; Halo-KDEL (Addgene; 124316) (30 (link)) was a gift from J. Wang; pEGFP-parkin WT (Addgene; 45875) (31 (link)) and pEGFP-parkin C431S (Addgene; 45877) (31 (link)) were gifts from E. Fon; EGFP-Rab7A (Addgene; 28047) (32 (link)) was a gift from Q. Zhong; pMXs-3xHA-EGFP-OMP25 (Addgene; 83356) (24 (link)) and pLJC5-Lamp1-RFP-3xHA (Addgene; 102932) (23 (link)) were gifts from D. Sabatini; psPAX2 (Addgene; 12260) was a gift from D. Trono; Lamp1-RFP (Addgene; 1817) (33 (link)) was a gift from W. Mothes; pGEX-4T-3-mR7BD (Addgene; 79149) (34 (link)) was a gift from A. Edinger; and Halo-TOMM20-N-10 (Addgene; 123284) was a gift from K. McGowan. Additional plasmids included the following: EGFP-Rab7 K38R (a gift from P. Song) (9 (link)), pLP3 (Invitrogen; K497500), and pGEX-4T-1 (Millipore Sigma; GE28-9545-49). The following plasmids were generated for this study using standard cloning procedures: LAMP1-Halo, LAMP1-pmTurquoise2, mCitrine-TOMM20, Halo-Parkin WT, Halo-Parkin C431S, pER4-mApple-TOMM20, pER4-LAMP1-mGFP, and pER4-3xHA-EGFP-OMP25.

Peng W., Schröder L.F., Song P., Wong Y.C, & Krainc D. (2023). Parkin regulates amino acid homeostasis at mitochondria-lysosome (M/L) contact sites in Parkinson’s disease. Science Advances, 9(29), eadh3347.

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Fluorescent protein-tagged cell imaging

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Cells were grown overnight in 24-well plates at 37°C in a 5% CO 2 atmosphere. After reaching over 80% confluence, the plasmids mRuby-Clathrin (Addgene #55852), pEGFP-Sec23A (Addgene #66609), ZsGreen-Rab5 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.), EGFP-Rab7A (Addgene #28047), GFP-LAMP1 (Addgene #16290), EMTB-3XGFP (Addgene #26741), pSNAPf-Cox8A (Addgene #101129), or pSNAPf-TOMM20 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.) was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. After 24 h, the transfected cells were digested with trypsin-EDTA and seeded into Nunc Glass Bottom Dishes (Φ 12 mm, Thermo Fisher Scientific, Inc.) at a density of 1.5~2.0 × 104 per well in growth medium (150 µL). The cells were grown for an additional 12~24 h before incubation with the indicated probes.

Han Y., Zhang Z., Liu W., Yao Y., Chen Y., Luo X., Wang W., Xu Y., Liu X., Kuang C., & Hao X. (2020). Labeling subcellular structures in living specimens using live-cell incompatible dyes with excellent optical properties.

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