Anti α sma

Manufactured by Novus Biologicals

Sourced in United States

Anti-α-SMA is a laboratory reagent used for the detection and localization of α-smooth muscle actin (α-SMA) in various types of cells and tissues. It is a commonly used marker for the identification of myofibroblasts and smooth muscle cells.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Anti yap taz, by Cell Signaling Technology (1 mentions) Collagen 1, by Abcam (1 mentions) Chamber slide, by Thermo Fisher Scientific (1 mentions) Anti tgf β, by Abcam (1 mentions) Normal donkey serum (nds), by Jackson ImmunoResearch (1 mentions) Anti vimentin, by Novus Biologicals (1 mentions) Anti α sarcomeric actin, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

α-SMA

4 protocols using anti α sma

Immunolabeling of Primary Fibroblasts and Heart Tissue

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For immunolabeling, primary fibroblasts were seeded and grown on chamber slides (ThermoFisher Scientific) for 24 h, fixed in 4% paraformaldehyde (Sigma-Aldrich), blocked in 10% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA), then incubated overnight (at 4 °C) with anti-α-SMA, anti-vimentin (Novus Biologicals, Abingdon, UK), anti-TGF-β, and collagen 1 (Abcam, Hong Kong, China). Heart sections were incubated at 4 °C overnight with anti-α-SMA, anti-vimentin (Novus Biologicals), and anti-α-sarcomeric actin (Sigma-Aldrich). The primary antibody was revealed using respective anti-mouse IgG/IgM, anti-rabbit IgG, or anti-chicken IgG secondary antibody (Jackson ImmunoResearch). The nuclei were visualized via DAPI staining [12 (link)]. Samples were analyzed using a confocal microscope (LSM700, Zeiss, Jena, Germany).

Telesca M., Donniacuo M., Bellocchio G., Riemma M.A., Mele E., Dell’Aversana C., Sgueglia G., Cianflone E., Cappetta D., Torella D., Altucci L., Castaldo G., Rossi F., Berrino L., Urbanek K, & De Angelis A. (2023). Initial Phase of Anthracycline Cardiotoxicity Involves Cardiac Fibroblasts Activation and Metabolic Switch. Cancers, 16(1), 53.

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Immunodetection of Myofibroblasts

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Primary antibodies used: anti-αSMA (1:100, Novus Biologicals, Littleton, CO).

Makino K., Makino T., Stawski L., Mantero J.C., Lafyatis R., Simms R, & Trojanowska M. (2017). Blockade of PDGF receptors by crenolanib has therapeutic effect in patient fibroblasts and in preclinical models of systemic sclerosis. The Journal of investigative dermatology, 137(8), 1671-1681.

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Histological Analysis of Mouse Skin

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For histology, 5-μm sections of mouse skin biopsies were stained with Gomori trichrome (Chromaview, Dublin, OH). For immunohistochemistry slides were processed and stained as described before (Stawski et al. 2012 (link)). Primary antibodies used: anti-αSMA (1:100, Novus Biologicals), anti-YAP/TAZ (1:100, D24E4; Cell Signaling Technology).

Toyama T., Looney A.P., Baker B.M., Stawski L., Haines P., Simms R., Szymaniak A.D., Varelas X, & Trojanowska M. (2017). Therapeutic targeting of TAZ and YAP by dimethyl fumarate in systemic sclerosis fibrosis. The Journal of investigative dermatology, 138(1), 78-88.

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Histological Analysis of Pancreatic Tumor

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Primary and surrounding tissues of pancreatic tumor were fixed in 10% formalin and embedded in paraffin. Tissue slides were stained with haematoxylin and eosin and Sirius red (collagen deposition). For immunohistochemistry staining, anti-α-SMA (Novus, Cat. No. NBP1-30894) and cleaved-Caspase3 (Cell Signaling Tech. Cat. No. 9661) were used.

Hong E., Barczak W., Park S., Heo J.S., Ooshima A., Munro S., Hong C.P., Park J., An H., Park J.O., Park S.H., La Thangue N.B, & Kim S.J. (2023). Combination treatment of T1-44, a PRMT5 inhibitor with Vactosertib, an inhibitor of TGF-β signaling, inhibits invasion and prolongs survival in a mouse model of pancreatic tumors. Cell Death & Disease, 14(2), 93.

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