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Human sae cells from healthy subjects

Manufactured by Lonza
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Human SAE cells from healthy subjects are primary epithelial cells derived from small airway epithelium. They provide a model system to study lung biology and respiratory diseases.

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Lab products found in correlation

Control sirna, by Qiagen (2 mentions) Ddx58, by OriGene (2 mentions) Human recombinant ifn β protein, by Merck Group (2 mentions) Pcmv6 entry, by OriGene (2 mentions)

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SAE cells (4 citations)

2 protocols using human sae cells from healthy subjects

1

Silencing RNA Inhibition of IRF Pathways

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Monolayers of human SAE cells from healthy subjects (Lonza, Walkersville, MD) were submerged cultured. All cells are assayed and test negative for HIV-1, mycoplasma, Hepatitis-B, Hepatitis-C, bacteria, yeast and fungi upon isolation by Lonza. Cells were only used for experiments at passages 3–6 and at a confluency of approximately 70%. SAE cells were transfected by administering silencing RNA (siRNA) for MDA-5, RIG-I, Trif, MAVS, LGP2, IFNAR1, IFNAR2 or control siRNA (Qiagen, Gaithersburg, MD). Cells were treated with RSV at a MOI of 0.3 for 24 hours. Cells were also treated with mock control as described above. Additionally, SAE cells were transfected with either an empty vector (Origene, Rockville, MD; pCMV6-Entry) or with the same vector that overexpresses RIG-I (DDX58) as recommended by the manufacturers (Origene). Human recombinant IFN-β protein (EMD Millipore) was administered to SAE cells at 1000 Units (U) cells for 24 hours.
Foronjy R.F., Taggart C.C., Dabo A.J., Weldon S., Cummins N, & Geraghty P. (2014). Type I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors. Mucosal immunology, 8(1), 161-175.
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2

Silencing RNA Inhibition of IRF Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monolayers of human SAE cells from healthy subjects (Lonza, Walkersville, MD) were submerged cultured. All cells are assayed and test negative for HIV-1, mycoplasma, Hepatitis-B, Hepatitis-C, bacteria, yeast and fungi upon isolation by Lonza. Cells were only used for experiments at passages 3–6 and at a confluency of approximately 70%. SAE cells were transfected by administering silencing RNA (siRNA) for MDA-5, RIG-I, Trif, MAVS, LGP2, IFNAR1, IFNAR2 or control siRNA (Qiagen, Gaithersburg, MD). Cells were treated with RSV at a MOI of 0.3 for 24 hours. Cells were also treated with mock control as described above. Additionally, SAE cells were transfected with either an empty vector (Origene, Rockville, MD; pCMV6-Entry) or with the same vector that overexpresses RIG-I (DDX58) as recommended by the manufacturers (Origene). Human recombinant IFN-β protein (EMD Millipore) was administered to SAE cells at 1000 Units (U) cells for 24 hours.
Foronjy R.F., Taggart C.C., Dabo A.J., Weldon S., Cummins N, & Geraghty P. (2014). Type I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors. Mucosal immunology, 8(1), 161-175.
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