Enzyme linked chemiluminescence ecl western detection reagents

Dasatinib was purchased from LC Laboratories. Roswell Park Memorial Institute (RPMI)‐1640 medium, Dulbecco's modified Eagle's medium (DMEM), foetal bovine serum (FBS), penicillin‐streptomycin and phosphate‐buffered saline (PBS) were bought from WelGENE. 3‐(4,5‐dimethylthiazol‐2‐y)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) reagent was from Promega. Bradford reagent was from Bio‐Rad. Protease inhibitor cocktail (PIC, 100×) and z‐VAD‐fmk (627610) were purchased from Calbiochem. Control siRNA (sc‐37007) and Src (sc‐29228) were obtained from Santa Cruz Biotechnology. Enzyme‐linked chemiluminescence (ECL) Western detection reagents were bought from Thermo Scientific. PCR primers were purchased from Bioneer. LY294002 and PD98059 were from Biomol Research Lab. Cell culture plastic wares were purchased from SPL Life Sciences. A detailed list of antibodies used in this study is included in Supplementary Table S1.

Roswell Park Memorial Institute Medium (RPMI)-1640 (LM011-01), Dulbecco’s Modified Eagles Medium (DMEM; LM001-05), fetal bovine serum (FBS; S001-01), penicillin-streptomycin (LS202-02) and phosphate-buffered saline (PBS) were purchased from Welgene (Daegu, Republic of Korea). PHA-665752 (A2307), SU11274 (A2678), JNJ38877605 (943540-75-8), and INCB28060 (A8448) were purchased from ApexBio Technology (Seoul, Republic of Korea). Doxorubicin (BML-GR319) was purchased from Enzo Life Science (Seoul, Republic of Korea). Antibodies and their dilution used in this study are mentioned in Table S1 as Supplementary File. Enzyme-linked chemiluminescence (ECL) Western detection reagents were bought from Thermo Scientific (Waltham, MA, USA). Cell culture plastic wares were purchased from SPL Life Sciences (Gyeonggi-do, Republic of Korea).