Histamine dihydrochloride

Manufactured by Bio-Techne

Sourced in Germany, United Kingdom

Histamine dihydrochloride is a chemical compound commonly used in various research applications. It serves as a precursor and intermediate in the synthesis of other compounds. This product can be utilized in various laboratory settings, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

α thrombin, by Enzyme Research (3 mentions) Dopamine hydrochloride, by Bio-Techne (2 mentions) Furimazine, by Promega (2 mentions) Ici 118 551, by Merck Group (2 mentions) Flat bottomed transparent microtiter plates, by Thermo Fisher Scientific (2 mentions) U46619, by Merck Group (2 mentions) Vuf 8430 dihydrobromide, by Bio-Techne (1 mentions) Jnj 10191584 maleate, by Bio-Techne (1 mentions) Thioperamide, by Bio-Techne (1 mentions) 3h namh, by PerkinElmer (1 mentions) Nluc substrate furimazine, by Promega (1 mentions) Poly d lysine, by Merck Group (1 mentions) Geneticin, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit and goat anti mouse antibodies, by Bio-Rad (1 mentions) Pf 3644022, by Bio-Techne (1 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (1 mentions) Polyclonal rabbit anti mlc, by Cell Signaling Technology (1 mentions) Fasudil, by LC Laboratories (1 mentions) Sb203580, by LC Laboratories (1 mentions) Vorapaxar, by Axon Medchem (1 mentions)

10 protocols using histamine dihydrochloride

Solubilizing Compounds for Pharmacological Studies

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VUF 8430 dihydrobromide and histamine dihydrochloride (Tocris Bioscience, Bristol, UK) were dissolved in saline (0.9% NaCl) immediately before use; GSK189254 (6-((3-cyclobutyl-2,3,4,5-tetrahydro-1H-3-benzazepin-7-yl)oxy)-Nmethyl-3-pyridinecarboxamide) (MedChemExpress, Monmouth Junction NJ, USA) and JNJ 10191584 maleate (Tocris Bioscience, Bristol, UK) were dissolved in 5% DMSO.

Borgonetti V, & Galeotti N. (2022). The Histamine H4 Receptor Participates in the Neuropathic Pain-Relieving Activity of the Histamine H3 Receptor Antagonist GSK189254. International Journal of Molecular Sciences, 23(22), 14314.

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Histamine Receptor Ligands Procurement

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Histamine dihydrochloride,
imetit dihydrobromide,
clobenpropit dihydrobromide, thioperamide, and pitolisant (BF 2649
hydrochloride) were purchased from Tocris Bioscience (Wiesbaden-Nordenstadt,
Germany). Poly-d-lysine and geneticin were obtained from
Sigma-Aldrich (Taufkirchen, Germany). [³H]NAMH (specific
activity: 79.7 Ci/mmol) was from PerkinElmer (Waltham, MA). Z27743747
and Z3303614736 were purchased from Enamine Ltd. (Kyiv, Ukraine).
The Nluc substrate furimazine and the HaloTag fluorescent dye NanoBRET
618 were obtained from Promega (Madison, WI). UR-PI294, VUF4903, VUF4904,
and VUF5207 were synthesized as described previously.^{27 (link),28 (link)} White-wall, white-bottomed 96-well microtiter plates were from Brand
(Wertheim, Germany) and Gibco (Waltham, MA).

Schihada H., Ma X., Zabel U., Vischer H.F., Schulte G., Leurs R., Pockes S, & Lohse M.J. (2020). Development of a Conformational Histamine H3 Receptor Biosensor for the Synchronous Screening of Agonists and Inverse Agonists. ACS Sensors, 5(6), 1734-1742.

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Molecular Mechanisms of Thrombosis and Angiogenesis

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α-Thrombin was purchased from Enzyme Research Laboratories. The murine PAR1 agonist peptide TFLLRN and the human PAR1 agonist peptide TFLLRNPNDK were synthesized and purified by reverse-phase, high-pressure liquid chromatography at Tufts University Core Facility. Histamine dihydrochloride was obtained from Tocris. VEGF was purchased from PeproTech. Rabbit IgG antibody and purified GST-HSP27 were obtained from Rockland Immunochemicals. The J2 HSP27 inhibitor was synthesized and purchased from ProbeChem. PF3644022 was purchased from Tocris. SB203580 was from LC laboratories. Vorapaxar was from Axon MedChem and Fasudil was from LC Laboratories. Polyclonal rabbit anti-p38, polyclonal rabbit anti-MK2, polyclonal rabbit anti-MK3, polyclonal rabbit phospho-MK2 (Thr³³⁴), polyclonal rabbit anti-MLC, polyclonal rabbit anti-MYPT1, polyclonal rabbit anti-phospho-MYPT1, monoclonal mouse anti-phospho-MLC, monoclonal mouse anti-HSP27, and polyclonal rabbit phospho-HSP27 (Ser¹⁵, Ser⁷⁸, and Ser⁸²) antibodies were purchased from Cell Signaling Technology. Monoclonal mouse anti-GAPDH antibody was from GeneTex. Monoclonal mouse anti-RhoA antibody was from Santa Cruz Biotechnology. HRP-conjugated goat–anti rabbit and goat–anti mouse antibodies were from Bio-Rad Laboratories.

Rada C.C., Mejia-Pena H., Grimsey N.J., Cordova I.C., Olson J., Wozniak J., Gonzalez D.J., Nizet V, & Trejo J. (2021). Heat shock protein 27 activity is linked to endothelial barrier recovery after proinflammatory GPCR-induced disruption. Science signaling, 14(698), eabc1044.

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Mechanosensitive Ion Channel Modulation

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Whole-cell patch clamp recordings were performed in parallel by two experimenters using either an Axopatch 200B amplifier or a Multiclamp 700A amplifier. Baseline mechanically activated currents were measured as described above using increasing 0.5-µm displacement increments until the stimulus-intensity–response relationship approached I_max. Histamine dihydrochloride (100 µM; Tocris) was delivered by gravity perfusion at a rate of 2–3 ml min⁻¹. Mechanically activated currents were assessed again in the presence of histamine, which elicited an inward current. Washout of histamine was performed over several minutes. Cells were finally perfused with 10 µM Yoda1 to assess whether the inactivation kinetics of the mechanically activated currents were slowed as has been previously shown for heterologous PIEZO1 currents²⁶.

Hill R.Z., Loud M.C., Dubin A.E., Peet B, & Patapoutian A. (2022). PIEZO1 transduces mechanical itch in mice. Nature, 607(7917), 104-110.

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Pharmacological Modulation of Neuroreceptors

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(RS)-Baclofen (BAC), sumatriptan succinate, [D-Ala², NMe-Phe⁴, Gly-ol⁵]-enkephalin (DAMGO), (-)–U50488, WIN 55-212, (1R, 4R, 5S, 6R)-4-Amino-2-oxabicyclo [3.1.0] hexane-4,6-dicarboxylic acid (LY379268), histamine dihydrochloride (HA), N⁶-Cyclopentyladenosine (CPA), all purchased from Tocris Bioscience. Picrotoxin (PTX) purchased from Sigma Aldrich. Benzyloxy-Cyclopentyladenosine (BnOCPA) was provided by the Hamm Lab.

Manz K.M., Zepeda J.C., Zurawski Z., Hamm H.E, & Grueter B.A. (2023). SNAP25 differentially contributes to Gi/o-coupled receptor function at glutamatergic synapses in the nucleus accumbens. Frontiers in Cellular Neuroscience, 17, 1165261.

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Neuroactive Compounds Acquisition Protocol

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Serotonin hydrochloride, Histamine dihydrochloride, Asenapine maleate were purchased from Tocris, Dopamine hydrochloride, Epinephrine hydrochloride, [Arg8]-Vasopressin acetate salt, Paliperidone, Somatostatin were obtained from Sigma-Aldrich, Aripiprazole was purchased from Toronto Research Chemical.

Galinski S., Wichert S.P., Rossner M.J, & Wehr M.C. (2018). Multiplexed profiling of GPCR activities by combining split TEV assays and EXT-based barcoded readouts. Scientific Reports, 8, 8137.

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Intranasal PEA-OXA for Neuropathic Pain Relief

Cited 2 times

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Forskolin (Cat. F6886), thapsigargin (Cat. T9033), medetomidine, idazoxan, and kolliphor were purchased from Sigma Aldrich (Milan, Italy). Histamine dihydrochloride (Cat. 3545), Immepip (Cat. 0932) were purchased from Tocris (UK). 2-Pentadecyl-2-oxazoline (PEA-OXA) was kindly provided by EPITECH Group SpA, Saccolongo (PD). PEA-OXA was dissolved in kolliphor 5% in saline. PEA-OXA (10 mg/kg) or vehicle (kolliphor 5%) were intraperitoneally daily administered starting 14 days after SNI or sham surgery for 16 days (day 30 from the SNI or sham surgery). The dosage of PEA-OXA was chosen based on previous studies of our and other laboratories [30 (link), 58 (link)].

Boccella S., Guida F., Iannotta M., Iannotti F.A., Infantino R., Ricciardi F., Cristiano C., Vitale R.M., Amodeo P., Marabese I., Belardo C., de Novellis V., Paino S., Palazzo E., Calignano A., Di Marzo V., Maione S, & Luongo L. (2021). 2-Pentadecyl-2-oxazoline ameliorates memory impairment and depression-like behaviour in neuropathic mice: possible role of adrenergic alpha2- and H3 histamine autoreceptors. Molecular Brain, 14, 28.

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Histamine Receptor Assay Protocol

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Histamine dihydrochloride was purchased from Tocris Bioscience (Wiesbaden-Nordenstadt, Germany). Poly-D-lysine (PDL), isoprenaline, ICI118.551 and U-46619 were obtained from Sigma Aldrich (Merck KGaA, Darmstadt, Germany). The Nluc substrate furimazine was from Promega (Madison, WI, USA) and white-wall, white-bottomed 96-well, as well as flat-bottomed transparent microtiter plates were from Gibco (Waltham, MA, USA).

Schihada H., Shekhani R, & Schulte G. (2021). Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Science signaling, 14(699).

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Histamine Receptor Assay Protocol

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Schihada H., Shekhani R., & Schulte G. (2021). Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors.

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Cocktail Stimulation of Neurotransmitters

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A cocktail was prepared freshly with AMPA (1 μM, Sigma-Aldrich), noradrenaline (1 μM, Sigma-Aldrich), kainic acid (kainate), ibotenic acid, serotonin, histamine dihydrochloride, dopamine hydrochloride, and N-methyl-D-aspartate (NMDA) (each 1 μM, from Tocris Bioscience), carbachol (10 μM, Sigma-Aldrich), and orexin (0.01 μM, Sigma-Aldrich). Distilled H 2 O was added in sister cultures as control in all stimulation protocols. To avoid differences in gene expression caused circadian rhythms, stimulations and RNA and protein extractions from different conditions were performed at the same circadian time. RNA was extracted three hours after stimulation and again 24 hours later (recovery).

Mikhail C., Vaucher A., Jimenez S, & Tafti M. (2017). ERK signaling pathway regulates sleep duration through activity-induced gene expression during wakefulness. Science signaling, 10(463).

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