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Rna hs assay kit

Manufactured by Thermo Fisher Scientific
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The RNA HS Assay Kit is a laboratory instrument designed for the quantification of low-abundance RNA samples. It utilizes fluorescent dye-based technology to detect and measure RNA concentrations with high sensitivity.

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Lab products found in correlation

Qubit fluorometer, by Thermo Fisher Scientific (9 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (8 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (8 mentions) Qubit 2, by Thermo Fisher Scientific (7 mentions) Qubit 3.0 fluorometer, by Thermo Fisher Scientific (7 mentions) 2100 bioanalyzer, by Agilent Technologies (6 mentions) Qubit 4 fluorometer, by Thermo Fisher Scientific (5 mentions) Hiseq 2500, by Illumina (4 mentions) Rneasy mini kit, by Qiagen (4 mentions) Allprep dna rna mini kit, by Qiagen (3 mentions) Novaseq 6000, by Illumina (3 mentions) Rneasy plant mini kit, by Qiagen (3 mentions) Recoverall total nucleic acid isolation kit, by Thermo Fisher Scientific (3 mentions) Rna 6000 pico assay kit, by Agilent Technologies (3 mentions) Nucleospin rna xs kit, by Macherey-Nagel (3 mentions) Qubit 4.0 fluorometer, by Thermo Fisher Scientific (3 mentions) Qubit 4.0 hs assay kit, by Thermo Fisher Scientific (3 mentions) Dsdna hs assay kit, by Thermo Fisher Scientific (2 mentions) Nanodrop 1000, by Thermo Fisher Scientific (2 mentions) Bioanalyzer 2100 system, by Agilent Technologies (2 mentions)

Spelling variants of this product

Qubit RNA HS Assay Kit (706 citations) RNA HS Assay (9 citations) Quant-iT RNA HS Assay Kit (6 citations) RNA HS Assay Kit with Qubit 3.0 Fluorometer (3 citations) Qubit RNA BR and HS Assay Kits (2 citations) Quant-iT Qubit RNA HS Assay Kit (2 citations) Qubit RNA HS (High Sensitivity) Assay Kit (2 citations) Qubit® 3.0 Fluorometer and RNA HS Assay Kit (2 citations) Qubit 2.0 Fluorometer (Qubit RNA HS Assay Kit (2 citations) Qbit RNA HS assay kit (2 citations)

61 protocols using rna hs assay kit

1

Extracting Nucleic Acids from FFPE Tissues

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For FFPE tissues, tumor-rich areas (>30-50% of neoplastic cells) were microdissected from three to six 4-μm unstained histologic sections under stereomicroscopic visualization with an Olympus SZ61 microscope (Olympus, Hamburg, Germany). Total nucleic acids were isolated from each target with the DNeasy Blood and Tissue kit on the automated QIAcube (Qiagen, Valencia, CA) instrument according to the manufacturer's instructions. Extracted DNA and RNA were quantitated on the Qubit 2.0 Fluorometer using the dsDNA HS Assay Kit and the RNA HS Assay Kit (Invitrogen, Carlsbad, CA).
Dacic S., Villaruz L.C., Abberbock S., Mahaffey A., Incharoen P, & Nikiforova M.N. (2016). ALK FISH patterns and the detection of ALK fusions by next generation sequencing in lung adenocarcinoma. Oncotarget, 7(50), 82943-82952.
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2

Quantifying Gene Expression in Drosophila Ring Glands

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Larvae were raised on NutriFly media and staged at 42 hr after the L2/L3 molt. 50 individual ring glands were collected per replicate, dissolved in Trizol, and stored at -80° for later qPCR analysis. RNA was extracted using Qiagen RNeasy extraction kit (74106) and concentrations were measured via the RNA HS assay kit (Invitrogen Q32852) in a Qubit 2.0 (Invitrogen Q10210). Extracted samples were reverse- transcribed via the ABI High capacity cDNA synthesis kit (ThermoFisher 4368814). Synthesized cDNAs were used for qPCR (QuantStudio 6 Flex, Applied Biosystems) using the Luna Universal qPCR master mix (NEB M3003S). Samples were normalized to rp49 by calculating fold changes via the ΔΔCT method.
Huynh N., Zeng J., Liu W, & King-Jones K. (2018). A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues. G3: Genes|Genomes|Genetics, 8(11), 3593-3605.
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3

Cytogenetic and Molecular Profiling of MDS

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Cytogenetic analysis was performed following standard procedure and G-banded karyotypes (7 ). FISH experiments on metaphase chromosomes were performed as previously described (8 (link)) using the following probes: Vysis CEP 1 (D1Z5, SpectrumOrange Probe, Abbott) for alphoid sequences of chromosome 1 and Vysis CEP 7 (D7Z1, SpectrumGreen Probe, Abbott) for chromosome 7 α-sat DNA (Fig. 1b). Analyses were carried out using a fluorescence microscope Olympus BX61 equipped with a highly sensitive camera JAI and driven by CytoVision 4.5.4 software. At least 7 abnormal metaphases were analyzed in each experiment.
Nucleic acids were extracted from unsorted bone marrow cells of patients using All Prep DNA/RNA Mini Kit (Qiagen), quantified with Qubit fluorimeter using Quant-i-T dsDNA HS Assay Kit and RNA HS Assay Kit (Invitrogen) respectively and samples quality was evaluated using Tapestation visualization (Agilent 2100 Bioanalyzer). Mutational diagnostic screening of 14 MDS-related genes was performed using DHPLC (Wavemaker software, Wave System, MD Transgenomic Inc., USA) and Sanger sequencing (3500 Genetic Analyzer, Applied Biosystems) (Table 1; Supplementary Table 1).
Fernandez A.G., Crescenzi B., Pierini V., Di Battista V., Barba G., Pellanera F., Di Giacomo D., Roti G., Piazza R., Adelman E.R., Figueroa M.E, & Mecucci C. (2019). A Distinct Epigenetic Program Underlies the 1;7 Translocation in Myelodysplastic Syndromes. Leukemia, 33(10), 2481-2494.
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4

RNA Isolation and RNA-Seq Library Preparation

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Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen, USA) after grinding plant material (100–150 mg) in liquid nitrogen. The amount of RNA was determined using a Qubit fluorometer (Invitrogen, USA) using the Qubit ™ RNA HS Assay Kit. To create barcoded RNA-Seq libraries, the NEBNext® Ultra ™ II RNA Library Prep Kit for Illumina® (New England BioLabs, USA) was used according to the manufacturer’s protocol. Sequencing of the obtained libraries was performed on a high-performance sequencer NovaSeq 6000 (Illumina, USA) using the NovaSeq 6000 S1 Reagent Kit v1.5 (300 cycles).
Gladysheva-Azgari M., Petrova K., Tsygankova S., Mitrofanova I., Smykov A., Boulygina E., Slobodova N., Rastorguev S, & Sharko F. (2022). A de novo genome assembly of cultivated Prunus persica cv. ‘Sovetskiy’. PLoS ONE, 17(6), e0269284.
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5

Ventral Root RNA Extraction and Normalization

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The RNA from ventral roots were obtained using TRI Reagent (Sigma-Aldrich), following the recommendations of the manufacturer. Quality and quantity of RNA was evaluated using spectrophotometry (Nanodrop 1000, Thermo Scientific) and fluorimetry (Qubit 2.0 and RNA HS Assay kit, Invitrogen, ThermoFisher Scientific). To be in comparable concentration as the RNA extracted from axons, each ventral root RNA was diluted down to 500 pg and then reisolated with the RNAqueous-Micro Total RNA Isolation Kit, following the procedure detailed above.
Farias J., Holt C.E., Sotelo J.R, & Sotelo-Silveira J.R. (2020). Axon microdissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons. RNA, 26(5), 595-612.
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6

Comprehensive RNA Extraction from Plant Tissues

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For each plant species, four infected samples and one mock inoculated sample were randomly selected out of ten replicates for total RNA extraction and downstream analysis using the different quantification techniques. For N. tabacum, RNA was extracted from 100 μl of homogenised leaf tissue using the RNeasy Plant Mini Kit (Qiagen), following the manufacturer's protocol (with on-column DNAse digestion). For C. quinoa, RNA was extracted from 200 μl of homogenised leaf tissue using the Direct-zol RNA miniprep kit (Zymo), following the manufacturer's protocol. For N. benthamiana, RNA was extracted from 100 μl or 200 μl of homogenised leaf tissue using the Direct-zol RNA miniprep kit (Zymo), following the manufacturer's protocol. For all samples, RNA was eluted in 40 μl RNase free water, and yield and purity were determined using the Nanodrop spectrophotometer and Qubit3 fluorometer (RNA HS Assay kit, Invitrogen). After extraction, multiple aliquots of RNA were stored at −80 °C.
Boezen D., Johnson M.L., Grum-Grzhimaylo A.A., van der Vlugt R.A, & Zwart M.P. (2023). Evaluation of sequencing and PCR-based methods for the quantification of the viral genome formula. Virus Research, 326, 199064.
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7

Lexogen Split-RNA Extraction Protocol

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The RNA was extracted via the Lexogen Split-RNA extraction kit (Lexogen, Vienna, Austria) according to the manufacturer’s instructions. The kit is based on phenol-chloroform extraction in acidic conditions, and we found this technique to yield the highest amount of RNA. RNA concentrations were measured via Qubit 4 fluorometer (Invitrogen, Carlsbad, CA, USA), using the RNA HS Assay kit (Invitrogen).
Mattei D., Ivanov A., van Oostrum M., Pantelyushin S., Richetto J., Mueller F., Beffinger M., Schellhammer L., vom Berg J., Wollscheid B., Beule D., Paolicelli R.C, & Meyer U. (2020). Enzymatic Dissociation Induces Transcriptional and Proteotype Bias in Brain Cell Populations. International Journal of Molecular Sciences, 21(21), 7944.
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8

Milk Whey Total RNA Extraction

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Total RNA was extracted from 300 μL of milk whey using a miRNeasy Micro Kit (Qiagen, Hilden, Germany) and following the manufacturer’s protocol. The RNA concentration was determined using a Qubit RNA HS Assay Kit on a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA).
Smulski S., Pszczoła M., Stachowiak M., Bilińska A, & Szczerbal I. (2023). Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland. Journal of Veterinary Research, 67(4), 583-591.
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9

Transcriptional Analysis of CQ and 5-FU Treatments

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To investigate how treatment with the combination of CQ and 5-FU changes tumor cell gene expression, we performed a transcription analysis of these cells. Total RNA was extracted using a QIAGEN RNeasy Plus Micro Kit. RNA was quantified using an RNA HS Assay Kit (Invitrogen) and a QBIT® system. The quality of RNA was analyzed using an Agilent RNA 6000 chip in a Bioanalyzer 2100 system. Only samples with an RNA integrity number higher than 8.0 (optimal quality) were processed.
All indications and steps of the Sure Select Strand-Specific RNA Library Preparation Kit were followed, and the dsDNA libraries of each treatment group with the adapters and index were analyzed on the Illumina Miseq platform. After obtaining the data, the reads were assembled based on the sequences of each transcript of interest using the CLC Genomics Workbench program. The effect of the treatments on gene expression was evaluated by comparing the fold change in RNA expression of the samples with that of the untreated control.
Zamame Ramirez J.A., Romagnoli G.G., Falasco B.F., Gorgulho C.M., Sanzochi Fogolin C., dos Santos D.C., Junior J.P., Lotze M.T., Ureshino R.P, & Kaneno R. (2020). Blocking drug-induced autophagy with chloroquine in HCT-116 colon cancer cells enhances DC maturation and T cell responses induced by tumor cell lysate. International Immunopharmacology, 84, 106495.
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10

Extracting DNA and RNA from Paraffin Sections

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DNA and RNA extraction was performed from 5 µm paraffin sections with a commercial kit following the manufacturer’s instructions (Wizard Genomic DNA purification Kit, Promega, Milano, Italia and RecoverAll Total Nucleic Acid Isolation Kit, Ambion, Austin, Texas). Total DNA and RNA were quantified with a fluorometer (Qubit 2.0, Invitrogen, Carlsbad, California) using a high-sensibility commercial kit (Qubit dsDNA HS or RNA HS Assay Kit, Invitrogen, Carlsbad, California) as per the manufacturer’s instructions.
Porcellato I., Brachelente C., Guelfi G., Reginato A., Sforna M., Bongiovanni L, & Mechelli L. (2014). A Retrospective Investigation on Canine Papillomavirus 1 (CPV1) in Oral Oncogenesis Reveals Dogs Are Not a Suitable Animal Model for High-Risk HPV-Induced Oral Cancer. PLoS ONE, 9(11), e112833.
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