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4 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride

1

Doxorubicin-Loaded Alginate Nanoparticles

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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), D, L-dithiothreitol (DTT), and polyvinyl alcohol (PVA, Mw: 72,000 Da) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Doxorubicin (DOX), a cationic anticancer drug, was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Alginate lyase was purchased from Hubei Xinmingtai Chemical Co., Ltd. (Wuhan, China). Alginate acid sodium salt (from brown algae, Mw: 12–58 kD, cell culture tested), dioctylsulfosuccinate (AOT), cystamine dihydrochloride (Cys), and gold(III) chloridehydrate (HAuCl4·3H2O) were purchased from Sigma–Aldrich (USA).
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2

Synthesizing Gold Nanoparticles with Functionalized Ligands

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Chloroauric acid, 4-mercaptobenzoic acid, ethanol, silver nitrate, trisodium citrate, ascorbic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, potassium chloride, sodium chloride, sodium dihydrogen phosphate dehydrate, disodium hydrogen phosphate dodecahydrate, sodium sulfide, quinol and N-hydroxysuccinimide were purchased from Sinopharm Group Chemical Reagent Co., Ltd. Ketamine and amphetamine standard were got from cerilliant Co., Ltd. Bovine serum albumin was obtained from Genview Co., Ltd. Calf serum was purchased from Shanghai Xiaopeng Biological Technology Co., Ltd. Carboxyl magnetic beads was got from Shanghai Taoyu International Trade Co., Ltd.
The experimental water was ultra-pure water.
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3

Synthesis and Characterization of Cellulose Nanocrystals-TPE Conjugates

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Cellulose nanocrystals were purchased from Shanghai ScienceK Ltd, China, which were synthesized by sulphuric acid hydrolysis of microcrystal cellulose. 4-(1,2,2-Triphenylvinyl)benzoicacid (TPE-COOH) was purchased from Xi'an Qiyue Biotechnology Co. Ltd, China. Epichlorohydrin, sodium hydroxide (NaOH), ammonium hydroxide (NH3·H2O), dimethyl sulfoxide (DMSO), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 4-dimethylaminopyridine (DMAP), as well as NaCl, MgCl2·6H2O, CaCl2, ZnCl2, Pb(NO3)2, HgCl2, FeSO4·7H2O, FeCl3·6H2O, CuSO4·5H2O, CoCl2·6H2O, BaCl2·2H2O, AlCl3, CdCl2 and MnSO4 were purchased from Sinopharm Chemical Reagent Co. Ltd, China. All reagents were analytical grade and received directly without any further purification. The deionized water was used to prepare all aqueous solutions.
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4

Bacterial Detection with Aptamer-Functionalized PEI

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Citric acid monohydrate (C6H8O7•H2O), N-hydroxy succinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Sinopharm Chemical Reagent Co. Ltd. (China). Polyethyleneimine (PEI) was purchased from Aldrich-Sigma Chemical Co. Ltd. (USA). E. coli O157:H7, Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) were purchased from China Center of Industrial Culture Collection (CICC). E. coli O157:H7 aptamer was purchased from Sangon Biotech Co. Ltd. (China) and its sequence was given as follows: 5′-COOH-GCA ATG GTA CGG TAC TTC CCC ATG AGT GTT GTG AAA TGT TGG GAC ACT AGG TGG CAT AGA GCC GCA AAA GTG CAC GCT ACT TTG CTA A-3′. All reagents were of analytical grade and used directly without any treatment. Ultrapure water (18.2 MΩ) was utilized throughout the experiment.
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