Anti gst cryptate

(Biotin-DPPLHS-pT-AI), and poloboxtide (MAGPMQS-pT-PLNGAKKK) peptides were obtained from Peptron (Daejeon, South Korea). Full-length Human Plk1 expressed as an N-terminal glutathione S transferase (GST)fusion protein was obtained from Carna (Kobe, Japan). Streptavidin-XL665 and anti-GST-cryptate were purchased from Cisbio (Codolet, France). Full-length Human Plk1 fused with enhanced green fluorescent protein (EGFP) was prepared from mitotic HEK293T lysates expressing EGFP-Plk1. Streptavidin-coated 384-well plates, horseradish peroxidase (HRP)-conjugated secondary antibodies, and a 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution were obtained from Thermo Fisher Scientific (Waltham, MA, U.S.A.). To detect bound EGFP-Plk1, an anti-Plk1 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). Purpurogallin and poloxin were purchased from TCI America (Portland, OR, U.S.A.) and Sigma-Aldrich (St. Louis, MO, U.S.A.), respectively. All other reagents including MgCl 2 , MnCl 2 , and dithiothreitol were obtained from Sigma-Aldrich.