Anti actin a5441

Mouse monoclonal antibodies used in this study were purchased from the following companies: anti-CD147 (306202) from BioLegend, anti-MMP1 (sc-58377) from Santa Cruz, anti-GFP (04363-24) from Nacalai tesque, anti-HA (MMS-101P) from Covance Research Products, and anti-Actin (A5441) from Sigma-Aldrich. Rabbit polyclonal anti-TRE17/USP6 (ab179751) and anti-LAMP1 (ab24170) antibodies were purchased from Abcam, and goat polyclonal anti-MMP9 (AF911) antibody was purchased from R&D Systems. All Alexa-Fluor-conjugated secondary antibodies were purchased from Invitrogen Molecular Probes.

Protein content was quantified in cellular extracts using the BCA assay (Pierce, Rockford, IL, USA). Twenty-five micrograms of protein extract were loaded onto SDS-PAGE gel and subjected to electrophoresis following standard immunoblotting techniques. The following primary antibodies and titres were used: anti-WT1 (antibody C19: sc-192, 1/200, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Actin (A5441, 1/10000, Sigma-Aldrich, Oakville, ON, Canada). Immunoreactive bands were detected using species-specific horseradish peroxidase-conjugated secondary antibodies (1/2000, Cell Signaling, Danvers, MA, USA) and visualized and analyzed using the GE Healthcare ECL Plus Western Blotting Detection Reagents and the BioRad Imager Scanner and software (GE Healthcare, Mississauga, ON, Canada).

The anti‐progerin (ab66587), anti‐lamin A (ab8980), anti‐aquaporin 1 (ab65837), anti‐Arl3b (ab136648), and anti‐pericentrin (ab4448) antibodies were purchased from Abcam. The anti‐lamin A/C antibody (for immunoblotting, customized) was generated by GeneTex (Taiwan). The anti‐emerin (sc‐15378), anti‐Arp2 (sc‐15389), anti‐WASP (sc‐8353), and anti‐CEP164 (used in Fig 7A, sc‐515403) antibodies were purchased from Santa Cruz Biotechnology. The anti‐acetylated tubulin (T6793), anti‐γ‐tubulin (T5326), anti‐TTBK2 (HPA018113), anti‐actin (A5441), and anti‐α–tubulin (T6199) antibodies were purchased from Sigma‐Aldrich. The anti‐nesprin 2 (PA5‐51933) and anti‐Arp3 (PA5‐80307) antibodies were purchased from Thermo Fisher Scientific. The anti‐CEP164 antibody (used in Fig 8A, 4533.00.02) was purchased from SDIX. The anti‐myosin Va antibody (NBP1‐92156) was purchased from Novus. The anti‐CP110 antibody (12780‐1‐AP) was purchased from ProteinTech. The anti‐keratin 8 antibody (AB 531826) was purchased from DSHB. The anti‐centrin antibody (04‐1624) was purchased from Merck Millipore. Phalloidin (conjugated with Alexa Fluor‐488 or ‐546), DNase I (conjugated with Alexa Fluor‐488), and fetal bovine serum (FBS) were purchased from Invitrogen. Cytochalasin D was purchased from Sigma‐Aldrich. SAG was purchased from Enzo.

Antibodies: anti-MEKK5 (F-9; SC-5294) and anti-ACK (A11; SC-28336) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), anti-NEDD4 (07–049) was from Millipore (Billerica, MA, USA), anti-GFP (MMS-118R) and anti-ubiquitin (P4G7; MMS-258R) were from BioLegend (San Diego, CA, USA), anti-Myc (MMS-150R) and anti-HA (MMS-101R) were from Covance (Princeton, NJ, USA), anti-tubulin (BS1699) was from Bioworld Technology (Shanghai, China), and anti-actin (A5441) was from Sigma-Aldrich (St. Louis, MO, USA). Protein A–Sepharose beads (P3391) and glutathione agarose (G-4510) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Secondary antibodies were HRP-conjugated goat anti-mouse (31430) and goat anti-rabbit IgG (31460) from ThermoFisher Scientific (Waltham, MA, USA). MG-132 was purchased from Sigma-Aldrich (St. Louis, MO, USA). The MEKK5 and luciferase (control) shRNA oligos were synthesized by Sangon Biotech (Shanghai, China) Company. The lung cancer cell lines A549 and NCI-H1650 were purchased from the American Type Culture Collection (ATCC).

The ethyl alcohol (pure (E7023) and FastStart Essential DNA Green Master (06402712001)) were obtained from Sigma-Aldrich (Darmstadt, Germany). The 16% formaldehyde (methanol-free) was obtained from Thermo Fisher Scientific (28906, Seoul, Republic of Korea). The μ-Dish 35mm image dishes with polymer coverslip bottoms were obtained from ibidi GmbH (Gräfelfing, Germany). N-acetyl-L-cysteine (A7250) and MitoTEMPO (SML0737) were obtained from Sigma-Aldrich (Darmstadt, Germany).
The antibodies against RAP1 were obtained from Bethyl laboratories, Inc. (A300-306A-9, MA, USA); the anti-TPP1 (ab195234), anti-TRF1 (ab10579), anti-p16 (ab211542), and anti-53BP1 (ab175933) were from Abcam (Cambridge, UK); the anti-POT1 (NB500-176) and anti-TRF2 (NB100-56506) were from Novus Biologicals (Littleton, CO, USA); the anti-TIN2 (PA5-67076) was from Invitrogen (Waltham, MA, USA); the anti-p53 (P6874), p16 (ab211542), and anti-actin (A5441) were from Sigma-Aldrich (Darmstadt, Germany); and the anti-P21 (2947), PINK1 (6946), Parkin (4211), autophagy sampler kit (4445), SirT3 (2627), SirT5 (8779), and Tom20 (42406) were obtained from Cell Signaling Technology (Danvers, MA, USA). Ginsenoside F1 was prepared as described in our previous report [34 (link)].