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5 protocols using gallic acid

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Antioxidant Capacity Evaluation Protocols

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Electrophoresis, DCFH (2′,7′-dichlorofluorescin diacetate), Folin-Ciocalteu, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)), and AAPH (2,2′-Azobis(2-methylpropionamidine) dihydrochloride) reagents were acquired from Sigma-Aldrich (São Paulo, Brazil). Catechin, quercetin, tiobarbituric acid (TBA), sulfuric acid, sodium carbonate, aluminum chloride, hexane, sodium hydroxide, methanol, and hydrogen peroxide were purchased from Merck (São Paulo, Brazil). Ascorbic acid and gallic acid of analytical degree were acquired from Dinâmica (São Paulo, Brazil).
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Characterization of Bioactive Compounds

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Caffeine (99% of purity), 1,1-diphenyl-2-picrilhhydrasyl and quercetin (95% of purity) were provided by Sigma-Aldrich (São Paulo, Brazil), methanol and aluminum chloride by Synth (São Paulo, Brazil), Folin–Ciocalteau, ethanol, gallic acid, algin, benzoic acid, glycerin and sodium hydroxide by Dinâmica (São Paulo, Brazil), acetonitrile by J.T. Baker (Phillipsburg, New Jersey, United States), acetic acid, sodium carbonate, tannic acid, lecithin, cetyl alcohol and sorbic acid by Êxodo Científica (Sumaré, São Paulo, Brazil), chlorogenic acid (95% of purity) by Fluorochem (Hadfield, United Kingdom) and persea gratissima (avocado) oil by Croda (Campinas, São Paulo, Brazil). All solvents and reagents used were of analytical grade.
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Araçá Film Production and Characterization

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Purple araçá (Psidium myrtoides) was acquired in the city of Dourados, Mato Grosso do Sul (latitude of 22°06′01.5′′ S and longitude of 54°43′04.7′′ W). For the film production, pea starch (Romariz, São Paulo, Brazil), glycerol (Vetec, Rio de Janeiro, Brazil), sorbitol (Dinâmico, São Paulo, Brazil), and polyethylene glycol 400 (Neon, São Paulo, Brazil) were used. For the analysis of active compounds, ethylic (Neon, 95%, Suzano, Brazil), Folin-Ciocalteau (Dinamica, Indaiatuba, Brazil), sodium carbonate (Impex São Paulo, Brazil), gallic acid (Dinâmica, Santa Catarina, Brazil), sodium acetate (Proquímicos, Rio de Janeiro, Brazil), potassium chloride (Synth, Sao Paulo, Brazil), hydrochloric acid (Vetec, Duque de Caxias, Brazil), aluminum trichloride (Synth, São Paulo, Brazil), and quercitin (Sigma-Aldrich, São Paulo, Brazil). For microbiological analysis, bacterial strains of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC WDCM00013), Brain Heart Infusion (BHI) broth (Prodimol Biotecnologia, Belo Horizonte, Brazil), and Mueller–Hinton agar (Kasvi, Paraná, Brazil) were used.
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Rice Starch-Based Bioplastic Synthesis

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White, red, and black rice (Armazém Santa Filomena, São Paulo, Brazil) were used as a source of starch. Sorbitol D (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil) and glycerol P.A. (Synth, São Paulo, Brazil) were used as plasticizer. Calcium chloride PA (Sigma-Aldrich, Saint Louiz, MI, USA), gallic acid (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil), acetone PA (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil), methanol PA (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil), ethanol PA (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil), DPPH (1,1-difenil-2-picril-hidrazil) (Sigma-Aldrich, Saint Louiz, MI, USA), ABTS [2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] (Sigma-Aldrich, Saint Louiz, MI, USA), and Folin–Ciocalteau reagent (Dinâmica Química Contemporânea LTDA, São Paulo, Brazil) were used for analyses.
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Guava Leaves Antioxidant Potential

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The leaves of the guava plant (Psidium guajava Linn. var. Pomifera) from the seedling bed was kindly donated by the Division of Parks and Conservation Units of the Secretariat for the Environment (Sorocaba, São Paulo, Brazil), ethanol (Synth, São Paulo, Brazil), hydrochloric acid (Neon, São Paulo, Brazil). Folin-Ciocalteau reagent (Dinâmica, São Paulo, Brazil), sodium carbonate (Dinâmica, São Paulo, Brazil), gallic acid (Dinâmica, São Paulo, Brazil). 2,2-diphenyl-1-picrylhydrazyl (Sigma-Aldrich, Steinheim, Germany), methanol (Chemco, São Paulo, Brazil). All reagents used were analytical grade.
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