The concentrations of the serum creatinine (Scr) and blood urea nitrogen (BUN) were determined using a creatinine assay kit (Bio Vision, Milpitas) and enzymatic assay kit (Sigma Diagnostics), respectively, according to the protocol provided by the manufacturer.
Creatinine assay kit
Manufactured by Abcam
Sourced in United States, United Kingdom, China
The Creatinine Assay Kit is a quantitative colorimetric assay designed to measure creatinine levels in various biological samples, including serum, plasma, urine, and cell culture media. The kit utilizes a simple, reliable, and sensitive method to determine creatinine concentration.
Automatically generated - may contain errors
Lab products found in correlation
Mouse albumin elisa kit, by Abcam (4 mentions)
Urea assay kit, by Abcam (3 mentions)
Mouse tnf α uncoated elisa kit, by Thermo Fisher Scientific (3 mentions)
Mouse il 6 uncoated elisa kit, by Thermo Fisher Scientific (3 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Alt activity assay kit, by Abcam (2 mentions)
Anti cdk2, by Santa Cruz Biotechnology (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti cdk4, by Santa Cruz Biotechnology (1 mentions)
Alt and ast assay kit, by Thermo Fisher Scientific (1 mentions)
Horse radish peroxidase conjugated anti rabbit and anti mouse igg, by Cytiva (1 mentions)
Anti p21waf1 cip1, by Santa Cruz Biotechnology (1 mentions)
Anti cyclin a, by Santa Cruz Biotechnology (1 mentions)
Anti cyclin d1, by Santa Cruz Biotechnology (1 mentions)
Bradford method, by Bio-Rad (1 mentions)
Mouse albumin elisa quantitation kit, by Fortis Life Sciences (1 mentions)
Coomassie brilliant blue, by Merck Group (1 mentions)
Au480, by Beckman Coulter (1 mentions)
Creatine kinase activity assay kit, by Abcam (1 mentions)
Flexstation 3 multi mode microplate reader, by Molecular Devices (1 mentions)
47 protocols using creatinine assay kit
1
Serum Creatinine and BUN Analysis
2
Serum Creatinine Quantification by Fluorimetry
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Creatinine concentration in serum was measured by the fluorimetry-based Creatinine Assay Kit (Biovision, Milpitas, CA, U.S.A.) as per manufacturer's protocol. The creatinine concentration was recorded as mg/dl of serum.
3
Evaluating Cell Cycle Regulation
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The following reagents and chemicals were purchased from the indicated suppliers: anti-β-actin antibody and BITC from Sigma (St. Louis, MO, USA); horseradish peroxidase-conjugated anti-rabbit and anti-mouse IgG from Amersham (Arlington Heights, IL, USA); creatinine assay kit from Bio Vision (Milpitas, CA, USA); ALT and AST assay kits from Thermo Fisher Scientific Inc. (Middletown, VA, USA). Antibodies (anti-p21WAF1/CIP1, anti-CDK2, anti-CDK4, anti-cyclin A, and anti-cyclin D1) were ordered from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
4
Urinary Protein and Creatinine Analysis
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Urine was collected from the mice in individual metabolic cages (CL-0355; CLEA). The amount of urinary protein excretion was measured by the Bradford method (Bio-Rad, Oakland, CA). Serum creatinine was measured using Creatinine Assay Kit manufactured by BioVision (Milpitas, CA).
5
Urine Protein Composition Analysis
6
Uric Acid and Creatinine Quantification
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Blood was centrifuged at 3000 rpm for 10 min and the serum was separated. The levels of uric acid in the urine and serum were determined using the uric acid assay kits (BioVision Inc., Milpitas, CA, USA), while the levels of creatinine in the urine and serum were determined using the creatinine assay kit (BioVision Inc., Milpitas, CA, USA).
7
Quantification of Urine Albumin and Renal Angiotensin II
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Urine albumin and creatinine concentrations were determined using a direct competitive ELISA kit (Albuwell, Exocell, Philadelphia, PA), and a creatinine assay kit (BioVision, Milpitas, CA), respectively. For the determination of renal Ang II concentrations, kidney was homogenized and purified with acetone and ether extraction, and radioimmunoassay of homogenized renal tissue was performed.
8
Feline Metabolic Acidosis and CKD
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Demographic data including age, breed, sex, and weight were recorded. The serum bicarbonate concentration was recorded and cats were classified as having metabolic acidosis if the concentration was ≤16 mEq/L. For the CKD cats, stage and substages (based on IRIS guidelines for serum creatinine concentrations, systolic blood pressure, and urine protein‐to‐creatinine ratio) were recorded, as well as whether or not they were fed a renal diet.
Urine ammonia and creatinine concentrations were measured using commercially available enzymatic assays (Ammonia Reagent Assay; Pointe Scientific, Canton, Michigan and Creatinine Assay Kit [ab204537]; Abcam, Cambridge, Massachusetts). The coefficient of variation (CV) and total observed error (TEobs) for both assays have been determined previously.
22 (link) The intra‐assay CV was 1.27%, the inter‐assay CV was 2.34%, and allowable total error (TEA) was 6.0% for ammonia. For creatinine, the intra‐assay CV was 1.27%, the inter‐assay CV was 2.54%, and the TEA was 3.5%.
Concentrations of Na+, K+, and Cl− in urine were measured using an indirect ion‐selective electrode (ISE) with a commercially available chemistry analyzer (AU480, Beckman Coulter). The UAG was calculated using the formula (Na+ + K+) − Cl− = UAG.
Urine ammonia and creatinine concentrations were measured using commercially available enzymatic assays (Ammonia Reagent Assay; Pointe Scientific, Canton, Michigan and Creatinine Assay Kit [ab204537]; Abcam, Cambridge, Massachusetts). The coefficient of variation (CV) and total observed error (TEobs) for both assays have been determined previously.
22 (link) The intra‐assay CV was 1.27%, the inter‐assay CV was 2.34%, and allowable total error (TEA) was 6.0% for ammonia. For creatinine, the intra‐assay CV was 1.27%, the inter‐assay CV was 2.54%, and the TEA was 3.5%.
Concentrations of Na+, K+, and Cl− in urine were measured using an indirect ion‐selective electrode (ISE) with a commercially available chemistry analyzer (AU480, Beckman Coulter). The UAG was calculated using the formula (Na+ + K+) − Cl− = UAG.
9
Maternal Exposure to Insecticides Study
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The study subjects were pregnant women in their second to the third trimester, who were randomly selected from participants of a research project of maternal exposure to insecticides. The study consent and protocols were reviewed and approved by the Ethical Committee of Tzu Chi General Hospital/University (No. IRB102–71, approved on 8 October 2015). Blood and urine samples were collected from each of the 30 subjects during a check-up visit. The blood sampling method was performed by intravenous sampling with an empty needle, and 15 mL of blood was drawn into a blood collection tube without anticoagulant, which was transferred and stored in an ultra-low temperature freezer at −80 °C for later processing and analysis.
Urine was collected in a temporary storage container, and 50 mL was taken and stored in a sealed bottle, temporarily stored in a household refrigerator at 4 °C during the visit and transferred to a −80 °C ultra-low temperature freezer prior to analysis. Each urine sample was tested with 100 μL of the abcam creatinine assay kit prior to extraction to confirm that the sample urine was normally metabolized for analysis.
Urine was collected in a temporary storage container, and 50 mL was taken and stored in a sealed bottle, temporarily stored in a household refrigerator at 4 °C during the visit and transferred to a −80 °C ultra-low temperature freezer prior to analysis. Each urine sample was tested with 100 μL of the abcam creatinine assay kit prior to extraction to confirm that the sample urine was normally metabolized for analysis.
10
Urinary Biomarkers of Tubular Injury
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At the end of the experiment, the rats were placed in metabolic cages (Nalgene, Rochester, NY, USA) to collect 24-h urine. Urine samples were centrifuged at 5000× g for 15 min to remove debris, and the supernatant was analyzed. The variables measured were diuresis, creatinine (Creatinine Assay Kit (ABCAM, Cambridge, MA, USA)), glucosuria (IL 300 plus, Clinical Chemistry Analyzer, Instrumentation Laboratory, Bedford, MA, USA), and the urinary NAG activity (spectrophotometric assay) in urine sample as markers of tubular injury.
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