Cobas e immunoassay analysers

Retrieved data include fasting blood samples at baseline and at 12 weeks of IVF therapy for all subjects. Female reproductive hormones (oestrogen and progesterone), insulin and TSH were measured with electrochemiluminescence immunoassay ECLIA, using Cobas E immunoassay analysers (Roche Diagnostics, Indianapolis, USA). Plasma glucose was measured by the enzymatic reference method with hexokinase-glucose-6-phosphate dehydrogenase. At 4 weeks following initiation of IVF hormonal therapy, a β-HCG test was performed to confirm pregnancy status. Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated as follows: (fasting plasma insulin x fasting plasma glucose)/405.[23 (link)] GDM screening was conducted at 28 weeks of gestation with the 2-h 75-g oral glucose tolerance test (OGTT). Diagnostic GDM levels suggested by the International Association of Diabetes and Pregnancy Study Groups were applied and included the following plasma glucose levels: 95 mg/dL (5.3 mmol/L) fasting; 180 mg/dL (10 mmol/L) at 1 h and 153 mg/dL (8.5 mmol/L) at 2 h post-OGTT.[24 (link)]