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4 protocols using total protein

1

Biochemical Analysis of Compounds

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Monosodium glutamate and other chemicals were obtained from Sigma Aldrich Chemical Co. (St Louis USA) except where otherwise stated. Cholesterol, triglyceride, total protein, estrogen and progesterone kits were obtained from Randox Laboratories, London, England.
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2

Phytochemical and Biochemical Analysis

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Methanol, acetic acid, quercetin, and gallic acid were purchased from Merck (Darmstadt, Germany). Sodium carbonate (Na2CO3), aluminum chloride, potassium acetate, and Folin-Ciocalteu’s reagent were procured from Sigma Aldrich, Inc (St Louis, MO). Isobutyl alcohol, magnesium carbonate (MgCO3), iron (III) chloride (FeCl3), acetone, chloroform, sodium nitroprusside, Methanol, and ferric chloride were source from BDH Chemical Ltd (Poole, England). Water used was glass distilled. Creatinine (Lot No. 315822), aspartate aminotransferase (AST; Lot No. 295800), alanine aminotransferase (ALT; Lot No. 295830), bilirubin (Lot No. 311158), uric acid (UA; Lot No. 309349), total protein (Lot No. 261368), and albumin (Lot No. 302605) kits were sourced from RANDOX Laboratories Ltd, Crumlin, Co, Antrim, UK. Kenxin refrigerated centrifuge model KX3400C was used, while a Jenway UV-visible spectrophotometer (Model 6305; Jenway, Barlo World Scientific, Dunmow, UK) was used to measure the absorbance.
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3

Comprehensive Biochemical Profiling Protocol

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Total cholesterol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin and direct bilirubin, total protein, albumin, urea, creatinine, calcium, sodium, chloride, potassium, and bicarbonate test kits were purchased from Randox Laboratory (United Kingdom).
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4

Hepatotoxicity Evaluation Protocol

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Acetaminophen, chloroform, and methanol used were obtained from Fam-lab Nigeria Limited, Akure, and Lixok-k chemicals, Akure, respectively. Alanine transaminase (ALT), aspartate transaminase (AST), gamma glutamyltransferase (GGT) and total protein, and bilirubin were obtained from Randox Laboratories Limited, UK.
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