Phytantriol was purchased from A & E Connock (Hampshire, England). Poloxamer 407 (Lutrol® F127) was obtained from BASF (Ludwigshafen, Germany) and 1,2-propandiol (Propylene Glycol, purity≥99.5%) from Merck (Darmstadt, Germany). CD8 Ovalbumin peptide (SIINFEKL, OVA257–264) and CD4 Ovalbumin peptide (ISQAVHAAHAEINEAGR, OVA323–339) and 5(6)-tetramethylcarboxy rhodamine-labelled OVA257–264 (TMR-SIINFEKL) was purchased from Mimotopes (Clayton, Australia). Monophosphoryl Lipid A from Salmonella Minnesota RE 595 was purchased from Sigma-Aldrich (Missouri, USA). Purified saponin (Quil-A) was purchased from Brenntag Biosector (Frederikssund, Denmark). All other chemicals were of analytical grade.
Poloxamer 407 lutrol f127
Manufactured by BASF
Sourced in Germany
Poloxamer 407 (Lutrol F127) is a nonionic block copolymer that consists of polyethylene oxide and polypropylene oxide segments. It is commonly used in various laboratory applications due to its unique physicochemical properties.
Automatically generated - may contain errors
Lab products found in correlation
Milli rx 20, by Merck Group (2 mentions)
Methanol, by Carl Roth (2 mentions)
Sorbitol, by Merck Group (1 mentions)
Phospholipon 85g, by Lipoid (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Propylene glycol, by Merck Group (1 mentions)
Transcutol p, by Gattefosse (1 mentions)
Xanthan gum, by Merck Group (1 mentions)
Polyethylene oxide peo, by Merck Group (1 mentions)
Poloxamer 188 lutrol f68, by BASF (1 mentions)
Crospovidone kollidon cl, by BASF (1 mentions)
Egg phosphatidylcholine epc, by Lipoid (1 mentions)
Hepes, by AppliChem (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Tetrahydrofurane thf, by Thermo Fisher Scientific (1 mentions)
5 protocols using poloxamer 407 lutrol f127
1
Formulation and Characterization of Peptide-Loaded Nanoparticles
2
Artemether Topical Formulation Development
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Artemether (ART) was procured from Ipca Laboratories Ltd., India. Gelucire ® 43/01 Pellets and Transcutol ® P were obtained from Gattefossé, France whereas Phospholipon ® 85G (P85G) was donated by Lipoid GmbH, Germany. Tween ® 80, sodium azide and sorbitol were obtained from Sigma-Aldrich, USA. Poloxamer 407 (Lutrol ® F-127; BASF, Ludwigshafen, Germany) and Carbopol ® 971P NF (The Lubrizol Corporation, USA) were equally gift samples. Propylene glycol, ethanol (Merck, Germany) and triethanolamine (Spectrum Chem. Mfg.
Corp., California) were procured from local suppliers. All reagents were of analytical grade and used without further processing. Bi-distilled water was used throughout the study.
Corp., California) were procured from local suppliers. All reagents were of analytical grade and used without further processing. Bi-distilled water was used throughout the study.
3
Formulation and Characterization of β-Lapachone
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β-Lapachone was supplied by the Mazence Pharmaceutical Company (Suwon, Korea). Crospovidone (Kollidon CL), poloxamer 407 (Lutrol F 127), and poloxamer 188 (Lutrol F 68) were obtained from BASF (Ludwigshafen, Germany). Poly(acrylic acid) (Carbopol 940) was procured from Lubrizol Advanced Materials Inc. (Cleveland, OH, USA). Xanthan gum, hydroxypropyl methylcellulose (HPMC), sucrose, and poly(ethylene oxide) (PEO) were purchased from the Sigma-Aldrich Company (St. Louis, MO, USA). Polyethylene glycol (PEG) 4000 was obtained from Daejung Chemicals and Metal Co., Ltd. (Siheung, Korea). Polydextrose was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Spray dried lactose was kindly provided by Whanin Pharm Co., Ltd. (Suwon, Korea). All other materials used were of pharmaceutical grade.
4
Poloxamer 407-Based Nanocarrier Extraction
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Poloxamer 407 (Lutrol F127) was from BASF AG (D-Ludwigshafen), cholesterol, sucrose, tween 80 and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide) were from Sigma-Aldrich (D-Steinheim), egg phosphatidyl choline (EPC) was obtained from Lipoid GmbH (D-Ludwigshafen), monoolein (GMOrphic-801) from Eastman Chemical Company (Kingsport, TN), methanol was from Carl Roth GmbH (D-Karlsruhe), ethanol and chloroform all from VWR International (D-Darmstadt) , and Hepes and sodium chloride were from AppliChem GmbH (D-Darmstadt). The cell lines were purchased from ATCC, USA. All cell culture materials were purchased from Lonza Bioscience (Morristown, USA). Puri ed water was prepared by ltration and deionization/reverse osmosis (Milli RX 20, Millipore, D-Schwalbach) Plant collection Ficus carica L. fam. Moraceae was collected from Wadi Fatima, Makkah, KSA and identi ed by Dr. Hany Gouda, Department of Pharmacognosy, Faculty of Pharmacy, Najran University. A voucher specimen (UQU-2019-1) of the plant is available in the herbarium, Department of Pharmacognosy, Faculty of Pharmacy, Umm al-Qura University. Dried plant leaves were powdered and extracted with petroleum ether. The obtained extract was evaporated and then subjected to separation using preparative HSCCC.
5
Lipid-based Nanoparticle Formulation
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Triglycerides trimyristin (D114, Dynasan 114) and Miglyol 812 were from Condea Chemie (D-Witten), Poloxamer 407 (Lutrol F127) was from BASF AG (D-Ludwigshafen), sodium glycocholate (SGC) and 5,10,15,20-tetrakis (4-hydroxyphenyl)-21H, 23H-porphine (porphyrin) were from Sigma-Aldrich (D-Steinheim), Lipoid S75 was from Lipoid GmbH (D-Ludwigshafen), monoolein (GMOrphic-801) from Eastman Chemical Company (Kingsport, TN), methanol was from Carl Roth GmbH (D-Karlsruhe), acetonitrile, ethanol and chloroform all from VWR International (D-Darmstadt), tetrahydrofurane (THF) was from Fisher Scientific (D-Nidderau), and Hepes and sodium chloride were from AppliChem GmbH (D-Darmstadt). Purified water was prepared by filtration and deionization/reverse osmosis (Milli RX 20, Millipore, D-Schwalbach).
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