SOD activity in the ear of the main stem was measured with the hydroxylamine method using a Total Superoxide Dismutase (T-SOD) assay kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s protocol. Main stem ear POD activity measurement was performed using a Peroxidase assay kit (Jiancheng Bioengineering Institute) according to the manufacturer’s protocol. For each treatment group, five samples were harvested (one plants randomly selected per pot, five pots per treatment group) and pooled, before following the assay protocol to obtain enzyme values.
Total superoxide dismutase t sod assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Total Superoxide Dismutase (T-SOD) assay kit is a laboratory equipment product manufactured by Nanjing Jiancheng. It is used to measure the total superoxide dismutase activity in samples.
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Lab products found in correlation
Peroxidase assay kit, by Nanjing Jiancheng (3 mentions)
Catalase cat assay kit, by Nanjing Jiancheng (3 mentions)
Malondialdehyde mda assay kit, by Nanjing Jiancheng (3 mentions)
Ascorbate peroxidase apx test kit, by Nanjing Jiancheng (2 mentions)
Total protein quantitative assay kit, by Nanjing Jiancheng (2 mentions)
Ldh activity assay kit, by Nanjing Jiancheng (1 mentions)
Hydrogen peroxide assay kit, by Nanjing Jiancheng (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Gemini em, by Molecular Devices (1 mentions)
Malondialdehyde assay kit, by Nanjing Jiancheng (1 mentions)
Catalase assay kit, by Nanjing Jiancheng (1 mentions)
Glutathione peroxidase, by Nanjing Jiancheng (1 mentions)
Reduced glutathione gsh assay kit, by Nanjing Jiancheng (1 mentions)
Total antioxidant capacity t aoc assay kit, by Nanjing Jiancheng (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
β actin, by Abcam (1 mentions)
Caspase 3, by Abcam (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Anti calreticulin rabbit pab, by ABclonal (1 mentions)
Enhanced atp assay kit, by Beyotime (1 mentions)
13 protocols using total superoxide dismutase t sod assay kit
1
Measuring Antioxidant Enzyme Activities
2
Membrane Permeability Assessment via LDH
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The lactate dehydrogenase (LDH) content can be used to determine the permeability of cell membranes. As the method used to measure the zeta potential, the cell pellets were exposed to aqueous ozone and were subsequently disrupted ultrasonically. The LDH content was then measured using an LDH activity assay kit (Nanjing Jiancheng Bioengineering Institute, China). Cells that were neither treated with aqueous ozone nor ultrasonically processed were used as the spontaneous group. Cells that were lysed directly by ultrasound were set as the maximum LDH released group. The damage ratio of the cell membrane was expressed by the following equation: A total superoxide dismutase (T-SOD) assay kit (Nanjing Jiancheng Bioengineering Institute, China) and a catalase (CAT) assay kit (Nanjing Jiancheng Bioengineering Institute, China) were used to measure the intracellular SOD and CAT activities, respectively. The measuring methods and equations were the same as those used for LDH in accordance with the manual.
3
Physiological Stability in Poplar Leaves
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Without the influence of external factors, the physiological indexes of the mature leaves (functional leaves) of poplar are relatively stable [71 (link)]. The 5th–7th healthy mature leaves of each plant from the four treatment groups were sampled and quickly placed in the ice box. Then, the physiological and biochemical indexes were measured in the laboratory immediately. A total of 0.2 g of each leaf sample was weighted and ground with 1.5 mL of the phosphate buffer. The homogenized liquid was transferred to a 5 mL tube for the determination of malonaldehyde (MDA), hydrogen peroxide (H2O2), Superoxide Dismutase (SOD), Catalase (CAT), and Ascorbate peroxidase (APX). The content of MDA was determined using the Thiobarbituric acid method, and the absorption values at 600 nm, 532 nm, and 450 nm wavelengths were recorded [72 (link)]. The contents of H2O2, SOD, CAT, and APX were determined with the Hydrogen Peroxide assay kit, Total Superoxide Dismutase (T-SOD) assay kit (Hydroxylamine method), Catalase (CAT) assay kit (Visible light), and Ascorbate peroxidase (APX) test kit (Nanjing Jian cheng Biological Engineering Institute, Nanjing, China), respectively. There were 9 biological replicates and 3 technical replicates for each treatment.
4
Mesalazine Modulates Colitis Pathways
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Mesalazine enteric-coated tablets (Sunflower Pharmaceutical Group Co., Ltd.); Dextran sodium sulfate (DSS, MW: 36000–50000, MP Biomedicals, USA); The myeloperoxidase (MPO) assay kit, total superoxide dismutase (T-SOD) assay kit (hydroxylamine method), malondialdehyde (MDA) assay kit (TBA method) and nitric oxide (NO) assay kit (nitrate reductase method) were purchased from Nanjing Jiancheng Bioengineering Institute; LXR-MultiDTM (Shanghai Universal Biotech Co., Ltd.); Occult blood test kit (Beijing Leagene Biotechnology Co., Ltd.); Primary antibodies against NLRP3, ASC, Caspase-1, JAK2, phospho-JAK2, STAT3, phospho-STAT3, IL-6, GAPDH and secondary antibody (goat anti-rabbit IgG, HRP-linked antibody) supplied by Cell Signaling Technology (Danvers, MA, USA);
5
Oxidative Stress Evaluation in C. elegans
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The CL4176 worms, which had become L4 larvae, were treated with GS-Rd for 2 days at 16°C. They were then warmed up to 25 °C and incubated for 24 h; about 1,000 worms were collected into 200 μL M9 Buffer in Eppendorf tubes. The worms were sonicated (Xinzhi, JY92-IIN) and pipetted into wells of 96-well plates containing a final concentration of 10 μM/L DCF-DA (Reactive Oxygen Species Assay Kit, Beyotime). Samples were read in a Gemini EM fluorescence microplate reader (Molecular Devices) at 37°C with an excitation of 485 nm and an emission of 530 nm. The remaining worm supernatant solution was used for the SOD assay (Total Superoxide Dismutase (T-SOD) assay kit, Nanjing Jiancheng) according to the instructions. Samples were read in a Gemini EM fluorescence microplate reader with an excitation of 550 nm.
6
Measuring Intracellular Superoxide Dismutase
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Intracellular SOD activity was measured by the Total Superoxide Dismutase (T-SOD) assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) based on the auto-oxidation of hydroxylamine. Targeted cells were lysed inPBS by ultrasonic pyrolysis (5 s sonication plus 5 s rest; 10 times). The homogenate was used for total SOD activity determination using thehydroxylamine method. The OD of the volume was obtained at 550 nm and each test was repeated over three times.
7
Cyanobacterial Stress Response Analysis
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Cyanobacterial cells were pelleted by centrifugation at 12,000 × g and 4 °C for 20 min and washed twice with PBS (50 mM, pH 7.8). After the pellets had been resuspended in PBS, the cells were disrupted using an Ultrasonic Cell Disruption System (NingBo Scientiz Biotechnological Co., Ltd, China) (200 W, ultrasonic time: 2 s; rest time: 8 s, 30 times) in an ice-bath. The supernatant was then collected by centrifugation for 20 min at 12,000 × g and 4 °C, and used to investigate the physiological changes, including antioxidase activity, total protein and malondialdehyde (MDA, a byproduct of lipid peroxidation) content. Total protein content, concentration of MDA and the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were determined using Total Protein Quantitative assay kit (No. A045-2), Malondialdehyde assay kit (No. A003-1), Total Superoxide Dismutase (T-SOD) assay kit (No. A001-1), Catalase assay kit (No. A007-1), and Peroxidase assay kit (No. A084-3) purchased from Nanjing Jiancheng Institute, China, respectively, according to the manufacturer’s instructions.
8
Antioxidant Enzyme Activity Assays
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The antioxidant enzyme activities were determined by using spectrophotometric method. For extraction of enzymes, the seedling samples were ground into powder in the liquid nitrogen, then suspended in the ice-cold phosphate buffer (0.1 M, pH = 7). The homogenates were vortexed for 1 min, then centrifuged at 4 °C for 15 min at 12,000 rpm. The supernatants were used for the determination of enzymatic activity. The superoxide dismutase (SOD) activity was determined according to the instructions of Total Superoxide Dismutase (T-SOD) assay kit (Jiancheng Bioengineering Institute). One unit of SOD activity was defined as the amount of enzyme required to cause a 50% inhibition of the reduction rate monitored at 550 nm. The ascorbate peroxidase (APX) activity was determined according to the Ascorbate Peroxidase (APX) test kit (Jiancheng Bioengineering Institute). The APX activity was determined based on the changes in absorbance at 290 nm (A10s and A130s). The catalase (CAT) and peroxidase (POD) activities were determined by using Catalase (CAT) Assay Kit and Peroxidase Assay Kit, separately (Jiancheng Bioengineering Institute).
9
Oxidative Stress Biomarkers Evaluation
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The serum concentration of malondialdehyde (MDA) and the serum activities of catalase (CAT), superoxide dismutase (SOD) and Glutathione Peroxidase (GSH-Px) were determined with Malondialdehyde (MDA) Assay Kit (thiobarbituric acid method), Catalase (CAT) Assay Kit (visible light), Total Superoxide Dismutase (T-SOD) Assay Kit (hydroxylamine method) and Glutathione Peroxidase (GSH-Px) Assay Kit (colourimetric method), which were supplied by Nanjing Jiancheng Bioengineering Institute (China).
10
Antioxidant and Anti-Inflammatory Assays
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The dimethyl sulfoxide (DMSO), lipopolysaccharide (Escherichia coli 0111: B6) and CUR were purchased from Sigma (Sigma, St Louis, MO, USA). Antimycotics, fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s high glucose medium were purchased from HyClone (HyClone, Logan, UT, USA). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo (Dojindo Laboratories, Kumamoto, Japan). The malondialdehyde (MDA) assay kit, total superoxide dismutase (T-SOD) assay kit, total antioxidant capacity (T-AOC) assay kit and reduced glutathione (GSH) assay kit were purchased from Jiancheng (Jiancheng, Nanjing, China). 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), annexin V and propidium iodide (PI) were purchased from Beyotime (Beyotime, Shanghai, China). For Western blot analysis, antibodies against HMOX1, NFE2L2, caspase-3 and β-actin were purchased from Abcam (Abcam, Cambridge, MA, USA), and antibody against caspase-9 was purchased from Affinity Biosciences (Affinity, Cincinnati, OH, USA). The ELISA kits used in the experiment were purchased from Sigma (Sigma, St Louis, USA).
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