Cell lysates were resolved by reducing SDS-PAGE, and assessed by standard western blotting. Antibodies used included: anti-GRHL2 (HPA004820) from Sigma-Aldrich; anti-E-cadherin (610182) from BD Transduction Laboratories; anti-ZEB1 (3396) and anti-ErbB3 (12708) from Cell Signaling Technology; anti-EPCAM/TROP1 (MAB960) and anti-TACSTD2/TROP2 (AF650) from R&D Systems; anti-Vimentin (M7020) from Dako; anti-β-actin (A1978) and anti-GAPDH (G9545) from Sigma-Aldrich. Secondary antibodies from Li-COR Biosciences were used: IRDye 800CW goat anti-mouse/rabbit (926-32210, 926-32211), IRDye 680LT goat anti-mouse/rabbit (926-68020, 926-68021) and IRDye 800CW donkey anti-goat (926-32214). Blots were scanned using the Odyssey Infrared Imaging System (Li-COR).
Anti e cadherin 610182
Manufactured by BD
Anti-E-cadherin (610182) is a laboratory equipment product. It functions as an antibody that specifically binds to the E-cadherin protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti vimentin m7020, by Agilent Technologies (2 mentions)
Odyssey infrared imaging system, by LI COR (2 mentions)
Anti grhl2 hpa004820, by Merck Group (2 mentions)
Anti β actin a1978, by Merck Group (2 mentions)
Irdye 800cw donkey anti goat, by LI COR (1 mentions)
Anti gapdh g9545, by Merck Group (1 mentions)
Anti e cadherin, by BD (1 mentions)
Irdye 680lt goat anti mouse rabbit, by LI COR (1 mentions)
Anti h3ac 06 599, by Merck Group (1 mentions)
2 protocols using anti e cadherin 610182
1
Western Blot Analysis of Epithelial-Mesenchymal Markers
2
Immunoblotting and Immunofluorescence Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For western blotting, primary antibodies used include: anti-GRHL2 (HPA004820) from Sigma-Aldrich; anti-E-cadherin (610182) from BD Transduction Laboratories; anti-ZEB1 (3396) from Cell Signaling Technology; anti-vimentin (M7020) from Dako; anti-β-actin (A1978) from Sigma-Aldrich; anti-H3K27me3 (ab6002) and anti-H3 (ab24834) from Abcam; anti-H3Ac (06-599) from Millipore. Secondary antibodies from Li-COR Biosciences were used: IRDye 800CW goat anti-mouse/rabbit (926-32210, 926-32211) and IRDye 680LT goat anti-mouse/rabbit (926-68020, 926-68021). Blots were scanned using the Odyssey Infrared Imaging System (Li-COR). Full blots are shown in Supplementary Fig. 15 . For immunofluorescence staining, primary antibodies used include anti-GRHL2 (HPA004820, Sigma-Aldrich) and anti-E-cadherin (610182, BD). Alexa Fluor 488-conjugated anti-rabbit (A11034) and Alexa Fluor 594-conjugated anti-mouse (A11032) from Invitrogen were used as secondary antibodies. Coverslips were mounted using Vectashield mounting medium with DAPI (4′,6-diamidino-2-phenylindole) (H-1200).
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