Amoxicillin, atropine, carbamazepine, dicloxacillin, digoxin, erythromycin, estradiol, furosemide, halothane, streptomycin, ticlopidine and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Allopurinol, azathioprine, diclofenac, diphenhydramine, flutamide, ibuprofen, imipramine, indomethacin, isoniazid, kanamycin, ketoconazole, metronidazole, nifedipine, phenobarbital, phenytoin, pioglitazone, sulfamethoxazole, troglitazone and valproic acid were purchased from Wako Pure Chemical (Osaka, Japan). Primidone was purchased from the Tokyo Chemical Industry (Tokyo, Japan). Primers were commercially synthesized at Life Technologies (Carlsbad, CA, USA). HaCaT cells were purchased from CLS Cell Lines Service (Eppelheim, Germany). CnT-Prime (CnT-PR) Epithelial Culture Medium and CnT-Prime 2D Diff (CnT-PR-D) Epithelial Culture Medium were from CELLnTEC Advanced Systems (Bern, Switzerland). All other chemicals and solvents were of analytical grade or the highest grade commercially available.
Nifedipine
Manufactured by Fujifilm
Sourced in Japan
Nifedipine is a laboratory equipment product manufactured by Fujifilm. It is a calcium channel blocker used in various research and analytical applications. The core function of Nifedipine is to regulate the flow of calcium ions across cell membranes, which can be important in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Indomethacin, by Fujifilm (2 mentions)
Nitrendipine, by Fujifilm (2 mentions)
Dicloxacillin, by Merck Group (1 mentions)
Allopurinol, by Fujifilm (1 mentions)
Azathioprine, by Fujifilm (1 mentions)
Diclofenac, by Fujifilm (1 mentions)
Diphenhydramine, by Fujifilm (1 mentions)
Flutamide, by Fujifilm (1 mentions)
Imipramine, by Fujifilm (1 mentions)
Isoniazid, by Fujifilm (1 mentions)
Ketoconazole, by Fujifilm (1 mentions)
Metronidazole, by Fujifilm (1 mentions)
Phenobarbital, by Fujifilm (1 mentions)
Phenytoin, by Fujifilm (1 mentions)
Pioglitazone, by Fujifilm (1 mentions)
Sulfamethoxazole, by Fujifilm (1 mentions)
Troglitazone, by Fujifilm (1 mentions)
Valproic acid, by Fujifilm (1 mentions)
Digoxin, by Merck Group (1 mentions)
Ibuprofen, by Fujifilm (1 mentions)
11 protocols using nifedipine
1
Comprehensive Pharmacological Compound Database
2
Nifedipine and NMDG Transport Assay
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Nifedipine, N-methyl-D-glucamine (NMDG) and 3-O-methylglucose were purchased from Wako Pure Chemical Industries, Ltd (Tokyo, Japan).
3
Glucose-stimulated Insulin Secretion in MIN6-K8 Cells
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MIN6‐K8 β‐cells18 were incubated for 30 min in Krebs–Ringer buffer containing 2.8 mmol/L glucose, and then stimulated for 240 min by 2.8 mmol/L glucose, 16.7 mmol/L glucose, 16.7 mmol/L glucose plus 10 μmol/L nifedipine (Wako, Osaka, Japan) or 2.8 mmol/L glucose plus 100 nmol/L glimepiride (Wako). In some experiments, cells were incubated in the presence of an insulin receptor antagonist, S961 (Phoenix Pharmaceuticals, Burlingame, CA, USA), at the concentration of 100 nmol/L throughout the experiments. Released insulin and insulin content were measured by an HTRF Insulin Kit (Cisbio Bioassays, Codolet, France), as previously reported19 .
4
Activators and Inhibitors of TRPC Channels
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The following TRPC3/C6/C7 activators were employed in the current study: L687 (Supplementary Figure S1 , WO/2022/118966) (29 ), Cannabidiol (CBD) (#Axon1234; Axon Medchem, VA, USA), and GSK1702934A (#6508; Tocris Bioscience, Bristol, UK). SKF96365 (#1147/10; R&D Systems, MN, USA) was used as the TRPC channel inhibitor. BAPTA-AM (#B035; DOJINDO, Kumamoto, Japan) was used as the Ca2+ chelator. EIPA (#14406; Funakoshi Co., Tokyo, Japan) and Cytochalasin D (#034-25881; FUJIFILM Wako Pure Chemical Co., Osaka, Japan) were used as the macropinocytosis inhibitors. Nifedipine (#141-05783; FUJIFILM Wako Pure Chemical Co., Osaka, Japan) was used as the L-type calcium channel blocker. UNC7938 (#AOB13597; AOBIOUS Inc., MA, USA) was used as the destabilizer of the endosomal membrane. All reagents were dissolved in anhydrous DMSO (#D12345; Thermo Fisher Scientific, MA, USA) and added to the medium at a final concentration of 0.3–1% (v/v).
5
Molecular Regulation of Myoblast Differentiation
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C2C12 myoblasts described previously (4 (link)) or mouse primary myoblasts were maintained and induced to differentiate into myotubes, and they were treated with STO-609 (Calbiochem), EGTA (Wako), GsMTx-4 (Peptide Institute), Yoda1, nifedipine (Wako), or tranilast (Tokyo Chemical Industry). Adenoviral vectors for LacZ and mouse KLF15 were described previously (35 (link)). Mouse KLF15 and negative control siRNAs were obtained from Invitrogen and were delivered into cells with the use of the Lipofectamine RNAiMAX transfection reagent (Invitrogen). Isolation of total RNA and quantitative RT-PCR analysis were performed as previously described (4 (link)). Data were normalized by the amount of 36B4 mRNA. The sequences of PCR primers are provided in Supplemental Tables 2 and 3 . Immunoblot analysis was performed with antibodies against STAT3 (4904, Cell Signaling Technology) and against Tyr705-phosphorylated STAT3 (9131, Cell Signaling Technology). Uncropped immunoblots are presented in Supplemental Figure 12 .
6
Nifedipine-Induced Oxidative Stress Assay
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Nifedipine [1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester], hydrogen peroxide (H2O2), and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Wako (Osaka, Japan). Dihydroethidium (DHE) was purchased from DOJINDO (Kumamoto, Japan). The anti-ICAM-1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The anti-mouse/rat AGT antibody was obtained from Immuno-Biological Laboratories (Takasaki, Japan).
7
Gingival Fibroblast Proliferation Modulation
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The effects of IL-1β (Fujifilm Wako Pure Chemical Corp., Osaka, Japan) and nifedipine (Fujifilm Wako Pure Chemical Corp.) on proliferation of human gingival fibroblasts were assessed using WST-8 tetrazolium (Cell Counting Kit-8, Dojindo, Kumamoto, Japan) by the MTT assay. Human gingival fibroblasts were seeded in 96-well plates and incubated for 24 h, after which the medium was changed as follows: 0.1, 1, or 10 µg/mL nifedipine, 10 ng/mL IL-1β + 0.1, 1, or 10 µg/mL nifedipine (0.1N, 1N, 10N, IL + 0.1N, IL + 1N, IL + 10N). After addition of WST-8 solution, cell proliferation was assayed by monitoring the absorbance at 450 nm over a period of 7 days using a spectrophotometer (SH-9000, Corona Electric Co., Ibaraki, Japan).
8
Photochemical and Pharmaceutical Compounds
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8-Methoxypsoralen (8-MOP) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anthracene, indomethacin, ketoprofen, naproxen, nifedipine, nimodipine, nitrendipine, promethazine, piroxicam, quinine, retinol, tamoxifen, pirfenidone, acetone, and sterilized 0.5 w/v% methyl cellulose (MC) solution were from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Acridin was from Cayman Chemical Company (Ann Arbor, MI, USA). Amlodipine and benzoyl peroxide were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Bufexamac, chlorpromazine, diclofenac, furosemide, gatifloxacin, and nalidixic acid were obtained from LKT Laboratories, Inc. (St Paul, MN, USA). Haloperidol was purchased from MP Biomedicals LLC (Santa Ana, CA, USA). Ibuprofen was from Polysciences Inc. (Warrington, PA, USA). Amiodarone, lomefloxacin, and sparfloxacin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dimethyl sulfoxide (DMSO) was from Dojindo Laboratories (Kumamoto, Japan).
9
Hydrogen Sulfide Signaling in Neurons
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Following drugs were used. Sodium hydrosulfide (NaHS) was purchased from Strem Chemicals (Newburyport, MA, USA). NaHS was used at concentrations from 0.03 mM to 3 mM because DRG neurons were sensitivity to more than 1 mM and RIN14B cells were stimulated by 0.1 mM to 1 mM (Miyamoto et al., 2011; Ujike et al., 2015) . Na 2 S 3 was purchased from Dojindo Molecular Technologies Inc (Kumamoto, Japan). Nifedipine (1 µM) (Wako, Osaka, Japan), ῳ-conotoxin GVIA (1 µM) (Peptide Institute, Osaka, Japan), ruthenium red (30 µM) (Wako Pure Chemical Industries, Osaka, Japan), HC030031 (0.3 mM), SB366791 (0.3 mM) (Bristol, UK), and trans-cinnamaldehyde (0.1-3 mM) (Aldrich Chemistry, USA) were prepared from stock solutions and dissolved in Hepes-buffered saline solution.
Na 2 S 3 (3-30 µM)-containing aqueous solution was made just before each experiment.
Na 2 S 3 (3-30 µM)-containing aqueous solution was made just before each experiment.
10
Reagents for Cellular Signaling Experiments
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Lactisole, monensin, 1,2-Bis(2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid tetrakis-acetoxymethylester (BAPTA/AM), 1,2-dioctanoyl-snglycerol (diC 8 ) and sucralose were obtained from Sigma-Aldrich (St. Louise, MO, U.S.A). Nifedipine, D-luciferin potassium salt, and N-methyl-D-glucamine (NMDG) were from Wako Pure Chemicals (Osaka, Japan). Forskolin was obtained from EMD Millipore (Billerica, MA, U.S.A). Fura-2/AM was obtained from Dojindo (Kumamoto, Japan).
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