Anti kif5b

Manufactured by Abcam

Anti-KIF5B is a protein that functions as a motor protein involved in intracellular transport and organelle movement. It is a member of the kinesin-1 family and plays a role in the movement of cargo along microtubules.

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Lab products found in correlation

Anti rab5, by Cell Signaling Technology (2 mentions) Anti lamp1, by Abcam (1 mentions) Folch fraction 1, by Merck Group (1 mentions) Anti β tubulin aa2, by Merck Group (1 mentions) Anti rab7 d95f2, by Cell Signaling Technology (1 mentions) Cell fractionation kit, by Cell Signaling Technology (1 mentions) Anti actin ac 74, by Merck Group (1 mentions) Anti histone h3, by Abcam (1 mentions) Anti ha ha 7, by Merck Group (1 mentions) Brain ps, by Avanti Polar Lipids (1 mentions) Egg pa, by Avanti Polar Lipids (1 mentions) Bovine liver phosphatidylinositol pi, by Avanti Polar Lipids (1 mentions) Non targeting control sirna, by Horizon Discovery (1 mentions) Anti eea1, by Cell Signaling Technology (1 mentions) Anti mhc mf20, by Developmental Studies Hybridoma Bank (1 mentions) Anti p p38 mapk, by Cell Signaling Technology (1 mentions) Anti ha epitope, by Thermo Fisher Scientific (1 mentions) Anti tubulin, by HuaAn Biotechnology (1 mentions) Anti myogenin f5d, by Developmental Studies Hybridoma Bank (1 mentions) Anti flag epitope, by Merck Group (1 mentions)

Spelling variants of this product

KIF5B (5 citations) Rabbit anti-KIF5B (5 citations)

3 protocols using anti kif5b

Endosomal Trafficking Lipid Composition

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DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), bovine liver phosphatidylinositol (PI), brain PS, and egg PA were purchased from Avanti Polar Lipids. PI 3-phosphate diC16 [PI(3)P], PI 4-phosphate diC16 [PI(4)P], and PI 5-phosphate diC16 [PI(5)P] were obtained from Echelon Biosciences. Folch fraction I and egg PC were purchased from Sigma-Aldrich. YM201636 PIKfyve inhibitor was purchased from Cayman Chemical. A cell fractionation kit was obtained from Cell Signaling Technology. Nontargeting control siRNA was obtained from Horizon Discovery, and human KLC1/2 siRNAs were from Santa Cruz Biotechnology. The following primary antibodies were used: horseradish peroxidase (HRP)–conjugated anti-His⁶ (71841, Novagen), anti-GFP for immunoblotting (3E1, Roche), anti-actin (AC-74, Sigma-Aldrich), anti–β-tubulin (AA2, Sigma-Aldrich), anti–histone H3 (Abcam), anti-giantin (PRB-114C, Covance), anti-HA (HA-7, Sigma-Aldrich), anti-KLC1 ([EPR12441(B)], Abcam), anti-KLC2 (Abcam), anti-KIF5B (Abcam), anti-LAMP1 (lysosome-associated membrane protein 1) (D2D11, Cell Signaling Technology), anti-Rab7 (D95F2, Cell Signaling Technology), and anti-Rab6 (D37C7, Cell Signaling Technology). Alexa 568– and Alexa 633–conjugated anti-mouse or anti-rabbit secondary antibodies were from Thermo Fisher Scientific.

Antón Z., Weijman J.F., Williams C., Moody E.R., Mantell J., Yip Y.Y., Cross J.A., Williams T.A., Steiner R.A., Crump M.P., Woolfson D.N, & Dodding M.P. (2021). Molecular mechanism for kinesin-1 direct membrane recognition. Science Advances, 7(31), eabg6636.

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Antibodies for Cellular Protein Analysis

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Antibodies used were the following: anti-EEA1 (#3288; Cell Signaling Technology, Danvers, MA), anti-Rab5 (#3547; Cell Signaling Technology), anti-p38MAPK (sc-7972; Santa Cruz Biotechnology, Dallas, TX), anti-pp38MAPK (#4511; Cell Signaling Technology), anti-MHC (MF-20; Developmental Studies Hybridoma Bank, Iowa City, IA), anti-myogenin (F5D; Developmental Studies Hybridoma Bank), anti-Bnip-2 (H-126; Santa Cruz Biotechnology), anti-KIF5B (ab42492; Abcam, Cambridge, MA), anti-JLP (ab12331; Abcam), anti-CDO (R&D systems), anti-FLAG epitope (F7425; Sigma-Aldrich, St. Louis, MO), anti-HA epitope (715500; Invitrogen), and anti-tubulin (M1301-3; HuaAn Biotechnology, Hangzhou, China).

Yi P., Chew L.L., Zhang Z., Ren H., Wang F., Cong X., Zheng L., Luo Y., Ouyang H., Low B.C, & Zhou Y.T. (2015). KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation. Molecular Biology of the Cell, 26(1), 29-42.

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Immunofluorescence Analysis of Wing Disc

Cited 1 time

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Third instar wing discs were staged and fixed in 4% paraformaldehyde. Immuno-fluorescence was performed as described(Das et al., 2013 (link)). Antibodies used: anti-pRET[Y905], anti-pJnk, anti-pAkt, anti-SOX2, anti-slug, anti-NCadherin, anti-pEGFR[Y845], anti-pEGFR[Y1068], anti-pFGFR[Y653/654], anti-Rab5, anti-Rab7, anti-Rab9(Cell Signaling), anti-pSRC[Y418] (Invitrogen), anti-dpERK (SIGMA), plus anti-Actin,, anti-E-cadherin, anti-α-Catenin, anti-Rho1, anti-Syntaxin, anti-β-tubulin (Developmental Studies Hybridoma Bank), anti-Actin, anti-GAPDH antibodies (Santa Cruz Biotechnology), anti-Rac1 antibody (BD biosciences), anti-KIF5B(Abcam), anti-EGFR (Julia Cordero), anti-Arp3(William Theurkauf).

Das T.K, & Cagan R.L. (2017). KIF5B-RET Oncoprotein Signals Through A Multi-Kinase Signaling Hub. Cell reports, 20(10), 2368-2383.

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