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2 protocols using polystyrene co maleic anhydride

1

Polystyrene-co-maleic anhydride cell culture

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Polystyrene co-maleic anhydride (molecular weight = ~1600), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), Hank’s balanced salt solution, Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, UAE). L-glutamine and an antibiotic solution of penicillin/streptomycin were purchased from (Merck Hertfordshire, UK). All consumable materials such as Petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 cm2 and 75 cm2), and dialysis tubing were purchased from (Merck Hertfordshire, 120 Moorgate London, UK).
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2

Cell Culture Reagents and Materials

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Dasatinib were retained from LC Laboratories (Woburn, MA, USA). Polystyrene co-maleic anhydride (molecular weight~1600), Roswell Park Memorial Institute (RPMI) 1640 medium, Hank’s balanced salt solution, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, United Arab Emirates). N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), L-glutamine, antibiotic solution of penicillin/streptomycin were acquired from (Merck Hertfordshire, UK). All consumable materials including petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 and 75 cm2), and dialysis tubing were purchased from (Merck Hertfordshire, UK).
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