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Mdm2 af1244

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MDM2 (AF1244) is a recombinant human protein that serves as a negative regulator of the tumor suppressor p53. The protein is produced in a baculovirus expression system and is purified by standard biochemical methods.

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Lab products found in correlation

Desmin, by Abcam (2 mentions) β actin ac 15, by Merck Group (2 mentions) P19arf, by Abcam (2 mentions) Pmdm2 s395, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

AF1244 (3 citations)

2 protocols using mdm2 af1244

1

Comprehensive Protein Analysis of Tissue Samples

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Tissue was homogenized using a Tissumizer from Tekmar in RIPA buffer and equal amounts of protein were separated by SDS-PAGE. The following antibodies were used for protein detection: MDM2 clone 2A10 (kind gift from Dr. Arthur Levine); MDM2 (AF1244) from R&D Systems; Gapdh (14C10), Pten (138G6), pAkt S473 (D9E) from Cell Signaling; Rela (C-20), Xiap (E-2), Slug (A-7) from Santa Cruz Biotechnology; p19Arf from Abcam; pMDM2 S395 from Thermo Fisher; and β-Actin (AC-15) from Sigma. Tissue specimens were stained with the following antibodies: B220 from BD Pharmingen; CD3, Myogenin (F5D), and Smooth Muscle Actin (1A4) from Dako; and Desmin from Abcam.
Comiskey DF J.r., Jacob A.G., Sanford B.L., Montes M., Goodwin A.K., Steiner H., Matsa E., Tapia-Santos A.S., Bebee T.W., Grieves J., La Perle K., Boyaka P, & Chandler D.S. (2017). A novel mouse model of rhabdomyosarcoma underscores the dichotomy of MDM2-ALT1 function in vivo. Oncogene, 37(1), 95-106.
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2

Comprehensive Protein Analysis of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was homogenized using a Tissumizer from Tekmar in RIPA buffer and equal amounts of protein were separated by SDS-PAGE. The following antibodies were used for protein detection: MDM2 clone 2A10 (kind gift from Dr. Arthur Levine); MDM2 (AF1244) from R&D Systems; Gapdh (14C10), Pten (138G6), pAkt S473 (D9E) from Cell Signaling; Rela (C-20), Xiap (E-2), Slug (A-7) from Santa Cruz Biotechnology; p19Arf from Abcam; pMDM2 S395 from Thermo Fisher; and β-Actin (AC-15) from Sigma. Tissue specimens were stained with the following antibodies: B220 from BD Pharmingen; CD3, Myogenin (F5D), and Smooth Muscle Actin (1A4) from Dako; and Desmin from Abcam.
Comiskey DF J.r., Jacob A.G., Sanford B.L., Montes M., Goodwin A.K., Steiner H., Matsa E., Tapia-Santos A.S., Bebee T.W., Grieves J., La Perle K., Boyaka P, & Chandler D.S. (2017). A novel mouse model of rhabdomyosarcoma underscores the dichotomy of MDM2-ALT1 function in vivo. Oncogene, 37(1), 95-106.
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