Murine OVCA cell line ID8 and human OVCA cell lines A2780.IP2, A2780.CP20 (platinum resistant), and SKOV3.IP were plated in 96-well tissue culture plates (Corning Costar, NY) at 2000 cells/well in 45μl RPMI + 10% FBS. The plates were incubated at 37 °C in a humidified 5% CO2 atmosphere and treated on day 2 with a single dose of entinostat (LC Laboratories, Woburn, MA), panobinostat (LC Laboratories), azacytidine (TOCRIS, Bristol, UK) or entinostat combined with azacytidine. Testing was done in duplicate assays with 8 replicates each. Cells were collected after either 24 or 72 hours of drug exposure and cytotoxicity was evaluated using the ATPlite luminescence-based assay (PerkinElmer, Waltham, MA) as previously described [33 (link)].
Turner T.B., Meza-Perez S., Londoño A., Katre A., Peabody J.E., Smith H.J., Forero A., Norian L.A., Straughn JM J.r., Buchsbaum D.J., Rand all T.D, & Arend R.C. (2017). Epigenetic modifiers upregulate MHC II and impede ovarian cancer tumor growth. Oncotarget, 8(27), 44159-44170.
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