Log In Sign up
The largest database of trusted experimental protocols

Cd25 pe cy5

Manufactured by BioLegend
Visit Supplier
Sourced in United States

CD25-PE/Cy5 is a fluorochrome-conjugated monoclonal antibody that binds to the human CD25 receptor. CD25 is expressed on activated T cells, B cells, and natural killer cells, and is a marker of immune cell activation. The PE/Cy5 fluorochrome combination provides a bright signal that can be detected using common flow cytometry instrumentation.

Automatically generated - may contain errors

Lab products found in correlation

Foxp3 efluor450, by Thermo Fisher Scientific (2 mentions) Live dead fixable yellow dead cell stain, by Thermo Fisher Scientific (2 mentions) Cd8 bv785, by BioLegend (1 mentions) Cd3 apc h7, by BD (1 mentions) Cd4 pe cy5, by BD (1 mentions) Il 22 apc, by Thermo Fisher Scientific (1 mentions) Il 2 percp cy5, by BioLegend (1 mentions) Ifn γ alexa fluor 700, by Thermo Fisher Scientific (1 mentions) Live dead fixable aqua blue, by Thermo Fisher Scientific (1 mentions) Il 17 alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Mip 1β pe cy7, by BD (1 mentions) Transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Gl7 efluor 660, by Thermo Fisher Scientific (1 mentions) Cd4 percp cyanine5, by BioLegend (1 mentions) Cd279 pd 1 pe, by BioLegend (1 mentions) Brilliant violet 711, by BioLegend (1 mentions) Cd4 percp cy5, by BioLegend (1 mentions) Cd3 apc cyanine7, by BioLegend (1 mentions) Cd107b alexa fluor 647, by BioLegend (1 mentions) Cd44 pe cyanine 7, by BioLegend (1 mentions)

Spelling variants of this product

CD25-PE (55 citations) Human Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD4 PE-Cy5/CD25 PE, (3 citations) PE/CY5-anti-mouse CD25 (2 citations)

4 protocols using cd25 pe cy5

1

Comprehensive Immunophenotyping of Antigen-Specific CD4 T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
6-day stimulated, CFSE-labeled PBMCs were subjected to three different panels of staining to measure: phenotypic and cytokine profile, HIV infectivity (p24) and intracellular transcription factors (T-bet and EOMES) in CFSE-low, Ag-specific CD4 T cells. Briefly, cells were first stained for viability with LIVE/DEAD Fixable Aqua Blue (Life Technologies), followed by surface makers staining, including CD3-APC-H7 (BD Bioscience), CD4-PE-Cy5 (BD Bioscience), CD8-BV785 (Biolegend), CD25-PE-Cy5 (Biolegend), CCR5-Pacific Blue (BD Bioscience), α4β7-APC (NIH AIDS Reagent Program), CCR6-APC (R&D Systems), CCR9-PerCP-Cy5.5 (Biolegend). After surface staining, cells were fixed, permeabilized (BD Bioscience) and subjected to intracellular staining, including HIV p24-PE (Beckman Coulter), IL-17-Alexa Fluor 488 (eBioscience), IL-22-APC (eBioscience), IL-2-PerCP-Cy5.5 (Biolegend), IFN-γ-Alexa Fluor 700 (eBioscience), MIP-1β-PE-Cy7 (BD Bioscience). Combination of surface and intracellular staining antibodies may vary according to different assays. For intracellular staining for T-bet and EOMES, cells were fixed and permeabilized using eBioscience’s transcription factor staining buffer set (eBioscience) according to manufacturer’s instructions. Cells were then subjected to intracellular staining with anti-T-bet-Pacific Blue (Biolegend) and anti-EOMES PE-eFluor 610 (eBioscience).
Liu F., Fan X., Auclair S., Ferguson M., Sun J., Soong L., Hou W., Redfield R.R., Birx D.L., Ratto-Kim S., Robb M.L., Kim J.H., Michael N.L, & Hu H. (2016). Sequential Dysfunction and Progressive Depletion of Candida albicans-Specific CD4 T Cell Response in HIV-1 Infection. PLoS Pathogens, 12(6), e1005663.
+ Open protocol
+ Expand
2

Multiparametric Flow Cytometry Analysis of T-Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
FOXP3‐eFluor 450 (eBioscience, Cat.# 48‐5773‐82, clone: FJK‐16s, 1:333), CD95 (Fas)‐Brilliant Violet 605 (BioLegend, Cat.# 152612, clone: SA367H8, 1:80), CD185 (CXCR5)‐Brilliant Violet 711 (BioLegend, Cat.# 145529, clone: L138D7, 1:40), CD4‐PerCP/Cyanine5.5 (BioLegend, Cat.# 100434, clone: GK1.5, 1:80), CD279 (PD‐1)‐PE (BioLegend, Cat.# 135206, clone: 29F.1A12, 1:20), CD25‐PE/Cy5 (BioLegend, Cat.# 102010, clone: PC61, 1:80), GL7‐eFluor 660 (eBioscience, Cat.# 50‐5902‐82, clone: GL7, 1:80), CD19‐APC/Cyanine7 (BioLegend, Cat.# 115530, clone: 6D5, 1:20), and LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher Scientific, Cat.# L34967, 1:7500).
Matos A.I., Peres C., Carreira B., Moura L.I., Acúrcio R.C., Vogel T., Wegener E., Ribeiro F., Afonso M.B., Santos F.M., Martínez‐Barriocanal Á., Arango D., Viana A.S., Góis P.M., Silva L.C., Rodrigues C.M., Graca L., Jordan R., Satchi‐Fainaro R, & Florindo H.F. (2023). Polyoxazoline‐Based Nanovaccine Synergizes with Tumor‐Associated Macrophage Targeting and Anti‐PD‐1 Immunotherapy against Solid Tumors. Advanced Science, 10(25), 2300299.
+ Open protocol
+ Expand
3

Multiparametric Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF‐α‐Brilliant Violet 421 (BioLegend, Cat.# 506328, clone: MP6‐XT22, 1:80), FOXP3‐eFluor 450 (eBioscience, Cat.# 48‐5773‐82, clone: FJK‐16s, 1:300), IL‐2‐Brilliant Violet 711 (BioLegend, Cat.# 503837, clone: JES6‐5H4, 1:40), IFN‐ɣ‐Brilliant Violet 785 (BioLegend, Cat.# 505838, clone: XMG1.2, 1:80), CD8a‐FITC (BioLegend, Cat.# 100706, clone: 53‐6.7, 1:50), CD4‐PerCP‐Cy5.5 (BioLegend, Cat.# 100434, clone: GK1.5, 1:80), CD25‐PE/Cy5 (BioLegend, Cat.# 102010, clone: PC61, 1:80), CD44‐PE/Cyanine7 (BioLegend, Cat.# 103029, clone: IM7, 1:80), IL‐10‐APC (BioLegend, Cat.# 505010, clone: JES5‐16E3, 1:80), CD107b‐Alexa Fluor 647 (BioLegend, Cat.# 108512, clone: M3/84, 1:200), CD3‐APC/Cyanine7 (BioLegend, Cat.# 100222, clone: 17A2, 1:80), and LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher Scientific, Cat.# L34967, 1:7500).
Matos A.I., Peres C., Carreira B., Moura L.I., Acúrcio R.C., Vogel T., Wegener E., Ribeiro F., Afonso M.B., Santos F.M., Martínez‐Barriocanal Á., Arango D., Viana A.S., Góis P.M., Silva L.C., Rodrigues C.M., Graca L., Jordan R., Satchi‐Fainaro R, & Florindo H.F. (2023). Polyoxazoline‐Based Nanovaccine Synergizes with Tumor‐Associated Macrophage Targeting and Anti‐PD‐1 Immunotherapy against Solid Tumors. Advanced Science, 10(25), 2300299.
+ Open protocol
+ Expand
4

Flow cytometric analysis of T cell subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells from all treatment groups were taken on the sixth day after treatment and centrifuged at 3000 rpm for 12 minutes. The pellets formed were used for examination using BD FACS Melody flowcytometry. The pellets were washed using PBS and centrifuged at 2500 rpm for 5 minutes. Staining using antihuman antibodies CD4+ FITC & IFN-γ PE for Th1, CD4+ FITC and IL-4 PE for Th2, CD4+ FITC and IL-17A PE for Th17, CD4+ FITC, CD25 PE/Cy5, and FoxP3 PE for Treg, and CD14 for macrophage markers (BioLegend, San Diego, CA, USA).
Raras T.Y., Fahrinda A., Y.u.l.i.a.t.i., Nurhidayati D.Y., Sujuti H, & Prawiro S.R. (2022). IMMUNE RESPONSE UPON THE ADMINISTRATION OF RECOMBINANT PROTEIN ANTIBODIES Ag-38 KDa Mycobacterium tuberculosis AND RIFAMPICIN EX-VIVO. African Journal of Infectious Diseases, 16(2), 71-79.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!