Clone g043h7
Clone G043H7 is a primary antibody that recognizes a specific epitope on its target antigen. It is designed for research use and can be used in various immunoassays to detect the presence and/or abundance of the target antigen in biological samples.
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10 protocols using clone g043h7
Multiparametric Flow Cytometry Sorting of T-cell Subsets
Monitoring T cell Activation and Phenotype
Flow Cytometry for Cell Phenotyping
Cell activation and phenotypes were determined by CD69 expression (Brilliant Violet 421 anti-human CD69 antibody; Clone FN50, BioLegend) and CCR7 and CD45RA expression (Pe/Cy7 anti-human CCR7 antibody; Clone G043H7, BioLegend and PerCP/Cy5.5 anti-human CD45RA antibody; Clone HI100, BioLegend). We had a minimum of 1000 events to be included as a data point in
Isolation and Sorting of Resting CD4+ T Cell Subsets from HIV-1-Infected Donors
Phenotypic Characterization of Activated T Cells
Expansion and Immunophenotyping of T Cells
Multiparametric Flow Cytometry Sorting of T-cell Subsets
Comprehensive Immune Profiling of TILs
For localization and quantification of granzyme B, cells were fixed (BLG420801, Biolegend), permeabilized (BLG421002, Biolegend), and stained with granzyme B-specific antibodies (BLG515406, BioLegend). For carboxy fluorescein succinimidyl ester (CFSE) cell proliferation assays, TIL cultures were stained before seeding at day 11 of the REP with 5 μM CFSE (Thermo Fisher Scientific) for 20 min at 37°C, according to the manufacturer’s instructions. Four days later cells were taken for flow cytometry analysis and their CFSE fluorescence intensity was determined. Samples were analyzed using FlowJo software (FlowJo LLC, Ashland, OR).
Isolation and Sorting of Resting CD4+ T Cell Subsets from HIV-1-Infected Donors
PBMC Isolation and Immunophenotyping
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