Toyopearl AF-Formyl-650M resin was from Tosoh Bioscience (King of Prussia, PA, USA). Wheat germ agglutinin (WGA) was purchased from Vector Laboratories (Burlingame, CA, USA). Stainless steel columns (2×250 mm) and the Gemini C18 column were obtained from Phenomenex (Torrance, CA, USA). Ovomucoid (type III-O, free of ovoinhibitor), trypsin (TPCK treated), PNGase F, chloroform, NaOH beads (97% purity), iodomethane, 2-mercaptoethanol, dithiothreitol, iodoacetamide, trizma base, and sodium cyanoborohydride (NaCNBH3) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Water and acetonitrile (ACN) were obtained from EMD Chemicals (Darmstadt, Germany). Formic acid was from Alfa Aesar (Ward Mill, MA, USA). Trifluoroacetic acid (TFA), dimethylformamide (DMF), urea, ammonium bicarbonate, sodium chloride, magnesium chloride, and calcium chloride were all from Mallinckrodt Pharmaceuticals (St. Louis, MO, USA). C18 Zip-tips were from Agilent Technologies (Santa Clara, CA, USA). C18 Sep-Pak cartridges were from Waters (Milford, MA, USA). Empty spin columns were purchased from Harvard Apparatus (Holliston, MA, USA).
Magnesium chloride
Manufactured by Mallinckrodt
Sourced in United States
Magnesium chloride is a chemical compound with the formula MgCl2. It is a white crystalline solid that is highly soluble in water. Magnesium chloride is commonly used as a laboratory reagent and is a component in various industrial processes.
Automatically generated - may contain errors
Lab products found in correlation
Calcium chloride, by Mallinckrodt (2 mentions)
Gemini c18 column, by Phenomenex (1 mentions)
Sep pak c18 cartridge, by Waters Corporation (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Pngase f, by Merck Group (1 mentions)
Wheat germ agglutinin (wga), by Vector Laboratories (1 mentions)
Iodomethane, by Merck Group (1 mentions)
Sodium chloride (nacl), by Mallinckrodt (1 mentions)
C18 zip tips, by Agilent Technologies (1 mentions)
Naoh beads, by Merck Group (1 mentions)
Sodium cyanoborohydride nacnbh3, by Merck Group (1 mentions)
Empty spin columns, by Harvard Apparatus (1 mentions)
Ammonium bicarbonate, by Mallinckrodt (1 mentions)
Sodium chloride (nacl), by Avantor (1 mentions)
Polyethylene glycol, by Hampton Research (1 mentions)
3 protocols using magnesium chloride
1
Glycoprotein Purification and Analysis
2
Anaerobic Protein Crystal Growth
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Protein stocks consisted of 30–35
mg/mL protein in a solution of 200 mM sodium chloride, 50 mM Tris/HCl
at pH 7.75, and 5 mM sodium dithionite. Protein crystals were grown
in 24-well plates using the sitting-drop method with a 1:1 ratio of
protein stock to reservoir solution at room temperature in an anaerobic
chamber with an atmosphere of ∼95% argon and ∼5% hydrogen.
All solutions were made anaerobic through a series of vacuum and argon
cycles. The reservoir solution for Av1 crystals consisted of double-distilled
water, 15% polyethylene glycol (MW 4000 g/mol, Hampton Research),
0.5–0.8 M sodium chloride (VWR), 0.2 M imidazole/malate at
pH 8 (Sigma-Aldrich), and 5 mM sodium dithionite (J.T. Baker). The
reservoir solution for Cp1 crystals consisted of double-distilled
water, 13.5–14% polyethylene glycol (MW 3350 g/mol, Hampton
Research), 0.3–0.5 M magnesium chloride (Mallinckrodt), 0.08
M Tris/HCl at pH 8 (Fisher Scientific), and 5 mM sodium dithionite.
Av1 and Cp1 crystals of block morphology formed overnight.
mg/mL protein in a solution of 200 mM sodium chloride, 50 mM Tris/HCl
at pH 7.75, and 5 mM sodium dithionite. Protein crystals were grown
in 24-well plates using the sitting-drop method with a 1:1 ratio of
protein stock to reservoir solution at room temperature in an anaerobic
chamber with an atmosphere of ∼95% argon and ∼5% hydrogen.
All solutions were made anaerobic through a series of vacuum and argon
cycles. The reservoir solution for Av1 crystals consisted of double-distilled
water, 15% polyethylene glycol (MW 4000 g/mol, Hampton Research),
0.5–0.8 M sodium chloride (VWR), 0.2 M imidazole/malate at
pH 8 (Sigma-Aldrich), and 5 mM sodium dithionite (J.T. Baker). The
reservoir solution for Cp1 crystals consisted of double-distilled
water, 13.5–14% polyethylene glycol (MW 3350 g/mol, Hampton
Research), 0.3–0.5 M magnesium chloride (Mallinckrodt), 0.08
M Tris/HCl at pH 8 (Fisher Scientific), and 5 mM sodium dithionite.
Av1 and Cp1 crystals of block morphology formed overnight.
3
Thiolated Oligonucleotides for Graphite-based Biosensors
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All thiolated oligonucleotides used in this study were purchased from Integrated DNA Technologies (Coralville, IA). Graphite powder (99%, 7–11 μM) was purchased from Alfa–Aesar (Heysham, Lancashire, UK). Sulfuric acid, phosphoric acid, hydrogen peroxide, trisodium citrate, sodium phosphate tribasic, sodium phosphate dibasic, boric acid, tetrachloroauric acid, dimethyl sulfoxide, apigenin, xylene, ethanol, 3,3-diaminobenzidine tetrahydrochloride, hematoxylin, and isopropanol were purchased from Sigma–Aldrich (Milwaukee, WI). Citric acid, calcium chloride, magnesium chloride, potassium permanganate, tris(hydroxymethyl)aminomethane (Tris), and hydrochloric acid were purchased from Mallinckrodt Baker (Phillipsburg, NJ). Alpha-MEM and fetal bovine serum were purchased from GIBCO (Campinas, Brazil). An MTT assay kit was purchased from Thermo Fisher Scientific (Waltham, MA). MUC1 (VU4H5) mouse mAb, anti-mouse IgG, and HRP-linked antibody were purchased from Cell Signaling Technology (Danvers, MA). Milli-Q ultrapure water (18 MΩ, Millipore, Billerica, MA) was used in all experiments.
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