Chlorpromazine chloride

All the chemicals and reagents used were of the highest purity available. HAuCl₄, Methylene Blue, Toluidine Blue O, RPMI 1640, XTT, Chlorpromazine chloride were procured from Sigma Aldrich, USA. Yeast extract, Peptone, dextrose and cotton buds were purchased from Himedia Laboratories Pvt. Ltd. All other reagents used were of analytical grade and procured from local suppliers.

To instigate oral candidiasis in the mice, a specific treatment and infection protocol were implemented. The animals underwent immunosuppression through two subcutaneous injections of prednisolone (Sigma Aldrich-P6004, Taufkirchen, Germany) at a dosage of 100 mg/kg. These injections were administered one day prior to and three days following the infection with Candida albicans ATCC 10231. Additionally, tetracycline hydrochloride (Sigma Aldrich-T7660, Taufkirchen, Germany) was introduced into the mice’s drinking water at a concentration of 0.9 mg/ml, starting one day before the infection. Anesthesia was induced through intramuscular injections of 2 mg/ml chlorpromazine chloride (Sigma), with 50 μl administered on each femur.
To initiate oral infections, small cotton pads (baby cotton buds from Johnson & Johnson) were immersed in a cell suspension of C. albicans ATCC 10231 containing 2.0 x 10⁸ viable cells per milliliter. These saturated cotton pads were then used to gently swab the entire oral cavity of the anesthetized mice. The daily assessment of infection severity relied on monitoring the presence and intensity of whitish, curd-like patches observed on the surface of the tongue [16 (link)].