Ot 2

Manufactured by Taconic Biosciences

The OT-II is a laboratory instrument designed for the generation and maintenance of transgenic mouse models. The core function of the OT-II is to facilitate the controlled manipulation and propagation of genetically engineered mouse strains.

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Lab products found in correlation

C57bl 6, by Taconic Biosciences (2 mentions) C57bl 6, by Charles River Laboratories (1 mentions) Bcl xltg mice, by Jackson ImmunoResearch (1 mentions) Cd2 icre mice, by Jackson ImmunoResearch (1 mentions) Rag1 mice, by Taconic Biosciences (1 mentions) Lck cre, by Jackson ImmunoResearch (1 mentions) Cd4 cre, by Taconic Biosciences (1 mentions)

9 protocols using ot 2

Transgenic Mouse Strains for Immunology Research

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C57BL/6 (H-2b) mice were obtained from the National Cancer Institute (Frederick, MD). OT-I (30 (link)) and OT-II (31 (link)) transgenic mice were purchased from Taconic Farms (Germantown, NY) and bred to Thy1.1⁺ background at Emory University. mOVA mice (C57BL/6 background, H-2b) (32 (link)) were a generous gift from Dr. Marc Jenkins (University of Minnesota, Minneapolis, MN). This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals. The protocol was approved by the Institutional Animal Care and Use Committee of Emory University (protocol number: DAR-2002050-092815GN). All surgery was performed under general anesthesia with maximum efforts made to minimize suffering. All animals were housed in specific pathogen-free animal facilities at Emory University.

Laurie S.J., Liu D., Wagener M.E., Stark P.E., Terhorst C, & Ford M.L. (2018). 2B4 Mediates Inhibition of CD8+ T Cell Responses via Attenuation of Glycolysis and Cell Division. Journal of immunology (Baltimore, Md. : 1950), 201(5), 1536-1548.

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Murine Models for T Cell Studies

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C57BL/6 (H-2^b) mice were obtained from the National Cancer Institute (Frederick, MD). OT-I (30 (link)) and OT-II (31 (link)) transgenic mice, purchased from Taconic Farms (Germantown, NY), were bred to Thy1.1⁺ background at Emory University. mOVA mice (C57BL/6 background, H-2^b) (32 (link)) were a gift from Dr. Marc Jenkins (University of Minnesota, Minneapolis, MN). All animals were maintained in accordance with Emory University Institutional Animal Care and Use Committee guidelines (Atlanta, GA). All animals were housed in pathogen-free animal facilities at Emory University.

Liu D., Burd E.M., Coopersmith C.M, & Ford M.L. (2016). Retrogenic ICOS Expression Increases Differentiation of KLRG-1hi CD127lo CD8+ T Cells During Listeria Infection and Diminishes Recall Responses. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1000-1012.

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Immunization with HIV-1 Epitope Peptides

Cited 1 time

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Mice were immunized subcutaneously (s.c.) with an immunodominant CTL epitope presented by H-2D^d in BALB/c mice, P18-I10 (RGPGRAFVTI) from the V3 loop of the IIIB strain of HIV-1 or PCLUS3-P18-I10 which contains both a CD4 helper epitope and CTL epitope (11 (link)) complexed in DOTAP. IL-1β (2 μg) was injected s.c.daily for 4 days where indicated(4 (link)). For the in vitro stimulation assays 6-12 week old C57BL/6 (Charles River), OT-I (Taconic) and OT-II (Taconic) mice were used.

Hogg A., Sui Y., Ben-Sasson S.Z., Paul W.E, & Berzofsky J.A. (2017). Role of CD4 T cell helper subsets in immune response and deviation of CD8 T cells. European journal of immunology, 47(12), 2059-2069.

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Genetic Mouse Models for Immunology

Cited 1 time

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HDAC3 fl/fl mice were provided by S. Hiebert (Vanderbilt (12 (link))). Human Bcl-2 Tg mice were generated by S. Korsmeyer (13 (link)) and provided by A. Singer (National Institutes of Health). Bcl-xL Tg mice (14 (link)), RORγt -KO mice (15 (link)), CD2-icre mice (16 (link)), and P2rx7-KO (17 (link))–(18 (link)) mice were purchased from The Jackson Laboratory. OT-II (19 (link)) mice were purchased from Taconic. Mice were housed in barrier facilities and experiments were performed at the Mayo Clinic with Institutional Animal Care and Use Committee approval. Mice were analyzed between the ages of 4 and 8 weeks with either littermate or age-matched controls (termed WT), which may include floxed only mice (no Cre), CD2-icre, or WT mice, as no differences were observed between these mice.

Philips R.L., McCue S.A., Rajcula M.J, & Shapiro V.S. (2019). HDAC3 protects DP thymocytes from P2X7 receptor-induced cell death. Journal of immunology (Baltimore, Md. : 1950), 202(4), 1033-1038.

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Mouse Strain Breeding and Characterization

Cited 1 time

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C57BL/6 (H-2b) and BALB/c (H-2d) mice were obtained from the National Cancer Institute (Frederick, MD). OT-I [19 (link)] and OT-II [20 (link)] transgenic mice, purchased from Taconic Farms (Germantown, NY), were bred to Thy1.1⁺ background at Emory University. mOVA mice (C57BL/6 background, H-2b) [21 (link)] were a gift from Dr. Marc Jenkins (University of Minnesota, Minneapolis, MN). 2B4 (CD244)^-/- animals on a B6 background [18 (link)] were a gift of Dr. Cox Terhorst (Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA), and were bred onto OT-I x Thy1.1 background at Emory University. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals. The protocol was approved by the Institutional Animal Care and Use Committee of Emory University (protocol number: DAR-2002050-092815GN). All surgery was performed under general anesthesia, and all efforts were made to minimize suffering. All animals were housed in pathogen-free animal facilities at Emory University.

Liu D., Suchard S.J., Nadler S.G, & Ford M.L. (2015). Inhibition of Donor-Reactive CD8+ T Cell Responses by Selective CD28 Blockade Is Independent of Reduced ICOS Expression. PLoS ONE, 10(6), e0130490.

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Mouse Lines for T Cell Studies

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C57BL/6 (H-2^b) and BALB/c (H-2^d) mice were obtained from the National Cancer Institute (Frederick, MD). OT-I (Hogquist et al., 1994 (link)) and OT-II (Barnden et al., 1998 (link)) transgenic mice, purchased from Taconic Farms, were bred to Thy1.1⁺ background at Emory University. mOVA mice (C57BL/6 background, H-2^b; Ehst et al., 2003 (link)) were a gift from M. Jenkins (University of Minnesota, Minneapolis, MN). 2B4 (CD244)^−/− animals on a B6 background were a gift from C. Terhorst (Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA), and were bred onto OT-I x Thy1.1 background at Emory University (Atlanta, GA). All animals were maintained in accordance with Emory University Institutional Animal Care and Use Committee guidelines. All animals were housed in pathogen-free animal facilities at Emory University.

Liu D., Krummey S.M., Badell I.R., Wagener M., Schneeweis L.A., Stetsko D.K., Suchard S.J., Nadler S.G, & Ford M.L. (2014). 2B4 (CD244) induced by selective CD28 blockade functionally regulates allograft-specific CD8+ T cell responses. The Journal of Experimental Medicine, 211(2), 297-311.

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Genetic Models of NFkB Signaling

Cited 2 times

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Bcl3^−/−, Nfkb2^−/−, Bcl3 transgenic and conditional Bcl-3^−/− mice have been described previously (Franzoso et al., 1998 (link); Franzoso et al., 1997 (link); Tassi et al., 2014 ; Zhang et al., 2013 (link)).. C57BL/6, OTII, Ly5.1 and Rag1^−/− mice were purchased from Taconic, Thy1.1, Lck-Cre, Nfkb1^−/− and IFNγ-YFP reporter mice from Jackson. Mice were housed in NIAID facilities, and all experiments were done with approval of the NIAID Animal Care and Use Committee and in accordance with all relevant institutional guidelines.

Tang W., Wang H., Claudio E., Tassi I., Ha H.L., Saret S, & Siebenlist U. (2014). The oncoprotein and transcriptional regulator Bcl-3 governs plasticity and pathogenicity of auto-immune T cells. Immunity, 41(4), 555-566.

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Generation of miR-210 Conditional Knockout Mice

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The Mir210 conditional knockout mice have been generated as described previously^{33 (link)}. Briefly, Mir210^f/f mice were generated by crossing miR-210 (lacZ-neo^r-flox) mice^{33 (link)} with the actin-Flp deleter mouse strain (Jax) to delete the lacZ–neomycin-resistance cassette, followed by backcrossing to the C57BL/6 (Jax) background for more than 9 generations. The C57BL/6 (Jax), Cd28^−/− (Jax), BoyJ (Taconic), CD45.1⁺CD45.2^–Rag2^−/− (Taconic), CD4-Cre (Taconic), OTII (Taconic) and P14 TCR transgenic (Jax) mouse strains were purchased from the indicated vendors. Il2^–/– D011.10 mice and Hif1a^f/f mice have been described previously^{50 (link), 51 (link)}. All mice were housed and bred in specific-pathogen-free conditions in the Animal Barrier Facility at the University of California, San Francisco. All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of California, San Francisco.

Wang H., Flach H., Onizawa M., Wei L., McManus M.T, & Weiss A. (2014). Negative Regulation of Hif1a Expression and TH17 Differentiation by Hypoxia Regulated miR-210. Nature immunology, 15(4), 393-401.

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Transgenic Mouse Models for Immunology

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Male C57BL/6 (H-2^b), BALB/c (H-2^d), and B6/Ly5.2 mice (H-2^b) (all 6–8 weeks) were obtained from the National Cancer Institute (Frederick, MD). OT-I ^{22 (link)} and OT-II ^{23 (link)} transgenic mice were purchased from Taconic Farms (Germantown, NY) and bred to Thy1.1⁺ mice at Emory. Transgenic mice expressing membrane-bound chicken ovalbumin under the control of the beta-actin promoter (C57BL/6 background, H-2b)^{24 (link)} were a generous gift from Dr. Marc Jenkins (University of Minnesota, Minneapolis, MN). Female B6.129(Cg)-Foxp3^{tm4(YFP/icre)Ayr} mice were purchased from Jackson Laboratories ^{25 (link)} (Stock No: 016959, Farmington, CT) and bred to TIGIT^fl/fl mice, a generous gift from Dr. Jane Grogan (Genentech). Recipients were either male or female, and donor animals for adoptive transfers and skin transplantation were sex matched to the recipient. This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals. The protocol (PROTO201700558) was approved by the Institutional Animal Care and Use Committee of Emory University.

Hartigan C.R., Tong K.P., Liu D., Laurie S.J, & Ford M.L. (2023). TIGIT agonism alleviates costimulation blockade resistant rejection in a Treg-dependent manner. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 23(2), 180-189.

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