Bassoon

Manufactured by Synaptic Systems

Sourced in Germany

The Bassoon is a laboratory instrument designed for the automated measurement and analysis of various samples. It features a precise and reliable system for handling and processing samples, enabling accurate and consistent data collection. The Bassoon's core function is to provide researchers and scientists with a versatile and efficient tool for their analytical needs.

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Lab products found in correlation

Neuronal nuclei (neun), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Vglut1, by Synaptic Systems (1 mentions) Vglut2, by Synaptic Systems (1 mentions) Glua1, by Merck Group (1 mentions) Calbindin, by Merck Group (1 mentions) Glun2a, by Merck Group (1 mentions) Sc12734, by Santa Cruz Biotechnology (1 mentions) Clone mab7a, by Synaptic Systems (1 mentions) Phospho erk2, by Santa Cruz Biotechnology (1 mentions) Sc 348, by Santa Cruz Biotechnology (1 mentions) Gephyrin, by Synaptic Systems (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Glun2b, by BD (1 mentions) Gapdh, by Merck Group (1 mentions) Gap43, by Novus Biologicals (1 mentions) Mms 435p, by Synaptic Systems (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Sc 6433, by Santa Cruz Biotechnology (1 mentions) Ab21679, by Abcam (1 mentions)

Spelling variants of this product

Anti-bassoon (6 citations) Guinea pig anti-Bassoon (2 citations) Rabbit anti-Bassoon (2 citations)

6 protocols using bassoon

Antibodies for Neurochemical Analysis

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Antibodies used for immunohistochemistry were as follows: vGlut1 (#135303, Synaptic Systems, Gottingen, Germany), vGlut2 (#135403, Synaptic Systems, Gottingen, Germany), bassoon (#141003, Synaptic Systems, Gottingen, Germany), tyrosine hydroxylase (#ab112, AbCam, Camdridge, MA) and NeuN (#MAB377; Millipore, Temecula, CA). For westerns, DOPA decarboxylase (#369003, Synaptic Systems, Gottingen, Germany), dopamine ß-hydroxylase (#ab209487, AbCam, Cambridge, MA), tryptophan hydroxylase 2 (#ab184505, AbCam, Cambridge, MA), dopamine D1 receptor (#ab216644, AbCam, Cambridge, MA), dopamine D2 receptor (#ab85367, AbCam, Cambridge, MA), dopamine transporter (#284003, Synaptic Systems, Gottingen, Germany), Lys63-linked ubiquitin (#05–1313, Millipore, Temecula, CA) and beta-actin (#a2228, Sigma, St. Louis, MO) antibodies were used.

Maynard M.E., Redell J.B., Kobori N., Underwood E.L., Fischer T.D., Hood K.N., LaRoche V., Waxham M.N., Moore A.N, & Dash P.K. (2019). Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory. Experimental neurology, 323, 113081.

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Antibody Characterization for Neuroscience Research

Cited 1 time

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In addition to the NRG2 antibodies described above, the commercial and custom antibodies against the following proteins were used in this study: ErbB4, mouse monoclonal Ab-77 (Thermo Fisher; 1 μg/ml), rabbit monoclonal mAB10 (1 μg/ml) ^{7 (link)} and rabbit polyclonal custom antibody 5721 (unpublished; 0.4 μg/ml); Kv2.1, mouse monoclonal (clone K89/34; NeuroMab; 1 μg/ml); rabbit polyclonal antibody against NRG1 (SC-348; Santa Cruz Biotechnology; 0.2 μg/ml); anti-V5 epitope tag (AbD Serotech; 1 μg/ml); Bassoon, rabbit polyclonal (Synaptic Systems; 1:1,000); Gephyrin, mouse monoclonal (clone mAb7a; Synaptic Systems; 1:500); PSD95, mouse monoclonal (clone 7E3-1B8; Pierce; 1:500); Calbindin, mouse monoclonal (clone CB-955; Sigma; 1:1,000); GFP, rabbit polyclonal (Molecular Probes; 1:2,000); Erk2, rabbit polyclonal (C-14; Santa Cruz; 0.2 μg/ml); phospho-Erk2, mouse monoclonal (clone E-4; Santa Cruz; 0.2 ug/ml); GAPDH, mouse monoclonal (clone 6C5; Santa Cruz; 0.2 μg/ml); clathrin heavy chain (CHC), mouse monoclonal (SC-12734; Santa Cruz; 0.1 μg/ml); GluN2A, rabbit monoclonal (clone A12W; Millipore; 1:2,000); GluN2B (clone 13; BD Biosciences; 1 μg/ml); GluA1 (AB1504; Millipore; 0.2 μg/ml).

Vullhorst D., Mitchell R.M., Keating C., Roychowdhury S., Karavanova I., Tao-Cheng J.H, & Buonanno A. (2015). A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling. Nature Communications, 6, 7222.

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Antibody Labeling Protocol for Protein Analysis

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Primary antibodies were purchased from Abcam [protein kinase C (PKCβ), DA receptor D1 (DR1)], Merck Millipore (GAP-43), Novus Biologicals [arrestin beta 2 (ARRB2), GAPDH], Sigma–Aldrich [ACTIN-β, fasciculation and elongation protein zeta 1 (FEZ1)], and Synaptic Systems (BASSOON). Secondary Alexa-coupled antibodies were from Life Technologies. Secondary HRP-coupled antibodies were from Dako. Anti-Mouse IgG + IgM/HRP was obtained from Jackson ImmunoResearch (West Grove, United States). Unless otherwise indicated, all other chemicals were obtained from Sigma–Aldrich.

Grabrucker S., Haderspeck J.C., Sauer A.K., Kittelberger N., Asoglu H., Abaei A., Rasche V., Schön M., Boeckers T.M, & Grabrucker A.M. (2018). Brain Lateralization in Mice Is Associated with Zinc Signaling and Altered in Prenatal Zinc Deficient Mice That Display Features of Autism Spectrum Disorder. Frontiers in Molecular Neuroscience, 10, 450.

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Immunohistochemical Analysis of Human Cortical Spheroids

Cited 1 time

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Cultured cells were fixed with 4% PFA for 10 minutes at room temperature, permeablized and blocked with 10% goat serum with 0.2% Triton-X100. For cryosections, hCS were fixed overnight at 4C in 4% PFA before transferring to 30% sucrose for 24 hours. Dehydrated hCS were then frozen in OCT and sliced at 10 μm thickness. Antibodies used were GFAP (DAKO, Z033401-2, dilution 1:1500), TUJ1 (Covance, MMS-435P, dilution 1:1500), Homer (Synaptic Systems, #160 011, dilution 1:1000), Bassoon (Synaptic Systems, #141 004, dilution 1:1000), HOPX (Santa Cruz, #sc-398703, dilution 1:300), phosphor-Vimentin (MBL, #D076-3, dilution 1:2000). Array tomography in hCS was performed as previously described (Pasca et al., 2015 (link)).

Sloan S.A., Darmanis S., Huber N., Khan T., Birey F., Caneda C., Reimer R., Quake S.R., Barres B.A, & Pașca S.P. (2017). Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells. Neuron, 95(4), 779-790.e6.

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Molecular Tools for Synaptic Protein Analysis

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Plasmids encoding for Syb2 (WT, rat) and Syt1 (rat) with a TEV protease cleavable superecliptic pHluorin-tag were a kind gift from J. Klingauf (University of Münster, Münster, Germany)7 (link). Mutant Syb2 (M46A) was cloned into a pHluorin plasmid by PCR amplification and standard cloning procedures. A PCR based approach was used to introduce a c-myc epitope tag into a plasmid encoding Syb2 (WT). The Syp-pHluorin plasmid (rat) was a kind gift from L. Lagnado (University of Sussex, UK)48 (link). A plasmid encoding Munc13-1-mCherry (rat) was derived from pEGFP-N1 carrying Munc13-1, kindly provided by N. Brose (MPI for Experimental Medicine, Göttingen, Germany). Clathrin light chain (rat) was fused to mRFP and expressed from pcDNA5/FRT/TO and fused to eGFP, expressed from pcDNA3. The plasmid encoding for Lifeact-eGFP57 (link) was a kind gift from F. Bradke (Center for Neurodegenerative Diseases, Germany). The CALM or scrambled shRNA was coexpressed with mKate from the pFUGW vector. The following antibodies were used: AP180 (155003, Synaptic Systems, 1: 100), Bassoon (141004, Synaptic Systems, 1: 500), CALM (sc-6433, Santa Cruz, 1: 100), clathrin heavy chain (ab21679, Abcam, 1: 400) c-myc epitope tag (9E10, purified from ascites, monoclonal, 1: 1,000).

Gimber N., Tadeus G., Maritzen T., Schmoranzer J, & Haucke V. (2015). Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins. Nature Communications, 6, 8392.

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Antibody Inventory for Brain Research

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The following primary antibodies were used: APP (6E10) (Novus Biotech, NBP2-62566, RRID:AB_2917960), Aβ (BioLegend, 803001, RRID:AB_2564653), β-actin (Cell Signaling Technology, 4970, RRID:AB_2223172), Bassoon (Novus Biologicals, NB120-13249), Bassoon (Synaptic Systems, 141016, RRID:AB_2661779), GFP (Millipore, 06-896, RRID:AB_310288), GFP (Invitrogen, A-6455), HA (Sigma-Aldrich, H6908, RRID:AB_260070), HA (Roche, 11867423001, RRID:AB_390918), Homer1, (Synaptic Systems, 160002, RRID:AB_2120990), Homer1, (Synaptic Systems, 160003, RRID:AB_887730), Homer1, (Synaptic Systems, 160006, RRID:AB_263122), LRP6 (Abcam, ab134146, RRID:AB_2895164), LRP6 (R&D Systems, AF1505, RRID:AB_2266025), LRP6 (Cell Signaling Technology, 2560, RRID:AB_2139329), LRP6 (Cell Signaling Technology, 3395, RRID:AB_1950408), pLRP6 (Cell Signaling Technology, 2568, RRID:AB_2139327), MAP2 (Abcam, ab5392, RRID:AB_2138153), MAP2 (Abcam, ab92434, RRID:AB_2138147), NeuN, (Cell Signaling Technology, 12943, RRID:AB_2630395), PSD-95 (Millipore, MAB1598, RRID:AB_94278), α-tubulin (Sigma-Aldrich, T9026, RRID:AB_477593), vGlut1 (Millipore, AB5905, RRID:AB_2301751), and vinculin (Sigma-Aldrich, V4505, RRID:AB_477617).

Jones M.E., Büchler J., Dufor T., Palomer E., Teo S., Martin-Flores N., Boroviak K., Metzakopian E., Gibb A, & Salinas P.C. (2023). A genetic variant of the Wnt receptor LRP6 accelerates synapse degeneration during aging and in Alzheimer’s disease. Science Advances, 9(2), eabo7421.

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