After SDS-PAGE, the proteins were transferred to nitrocellulose or low-fluorescent PVDF membrane and detected by immunoblotting using monoclonal antibodies or specific anti-sera, as indicated. Blots were washed with 1X PBST and incubated with anti-mouse or anti-rabbit HRP-conjugated or IR-680 and IR-800 dye-conjugated secondary antibodies (LI-COR Biotechnology, Lincoln, NE) for 1hr. Protein signals were detected with SuperSignal Western chemiluminescent substrate (Thermo Scientific, Inc., Waltham, MA) and developed with X-ray films according to the manufacturer’s instructions (Thermo Scientific, Inc) or via infrared detection of immunoblots using a LI-COR Odyssey 3000 infrared imager. Densitometric analysis was conducted using NIH ImageJ image analysis software 58 (link) or QuantityOne Software (Bio-rad).
Anti mouse or anti rabbit hrp conjugated or ir 680 and ir 800 dye conjugated secondary antibodies
Manufactured by LI COR
Anti-mouse or anti-rabbit HRP-conjugated or IR-680 and IR-800 dye-conjugated secondary antibodies are laboratory reagents used for detection and visualization of target proteins in various immunoassays and Western blotting applications. They bind to the primary antibody and provide a means for signal amplification or fluorescent labeling.
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2 protocols using anti mouse or anti rabbit hrp conjugated or ir 680 and ir 800 dye conjugated secondary antibodies
1
Immunoblotting Detection and Quantification
2
Immunoblotting Detection and Quantification
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After SDS-PAGE, the proteins were transferred to nitrocellulose or low-fluorescent PVDF membrane and detected by immunoblotting using monoclonal antibodies or specific anti-sera, as indicated. Blots were washed with 1X PBST and incubated with anti-mouse or anti-rabbit HRP-conjugated or IR-680 and IR-800 dye-conjugated secondary antibodies (LI-COR Biotechnology, Lincoln, NE) for 1hr. Protein signals were detected with SuperSignal Western chemiluminescent substrate (Thermo Scientific, Inc., Waltham, MA) and developed with X-ray films according to the manufacturer’s instructions (Thermo Scientific, Inc) or via infrared detection of immunoblots using a LI-COR Odyssey 3000 infrared imager. Densitometric analysis was conducted using NIH ImageJ image analysis software 58 (link) or QuantityOne Software (Bio-rad).
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