Anti β actin 200068 8f10

Manufactured by ZenBio

Anti-β-actin (200068-8F10) is a monoclonal antibody that recognizes the β-actin protein. β-actin is a ubiquitous cytoskeletal protein found in all eukaryotic cells. The antibody can be used to detect and quantify β-actin expression in various experimental applications.

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Lab products found in correlation

St506 2, by Beyotime (1 mentions) Anti aff4 14662 1 ap, by Proteintech (1 mentions) Anti collagen 1, by ZenBio (1 mentions) Anti runx2, by ZenBio (1 mentions) Anti alp, by ZenBio (1 mentions) Anti mtor, by ZenBio (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Immobilon western, by Merck Group (1 mentions) Ipvh00010, by Merck Group (1 mentions) Mouse anti flag sc 7392, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ha h6908, by Merck Group (1 mentions) Goat anti mouse igg hrp, by Proteintech (1 mentions) Hrp goat anti rabbit igg, by Proteintech (1 mentions) Pcr based mycoplasma detection kit, by Beyotime (1 mentions)

Spelling variants of this product

β-actin (30 citations) Anti-β-actin (8 citations) Anti-Actin (3 citations)

2 protocols using anti β actin 200068 8f10

Protein Extraction and Western Blot Analysis

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Protein contents were extracted with RIPA lysis buffer (P0013B; Beyotime) supplemented with 1% proteinase inhibitor phenyl‐methylsulfonyl fluoride (PMSF; ST506‐2; Beyotime). Isolated target protein on SDS‐PAGE gels were transferred onto PVDF membranes (IPVH00010; Millipore). The blotting membranes were treated with 5% skim milk for 1 h and then with primary antibody overnight (12–16 h). Thereafter, blotting bands were incubated with secondary antibodies at room temperature for 2 h before visualization with Immobilon® Western (P90719, Millipore). In this study, primary antibodies (1:1000) included: anti‐β‐actin (200068‐8F10; ZEN‐BIO), anti‐AFF4 (14662‐1‐AP; Proteintech), anti‐LC3B (18725‐1‐AP; Proteintech), anti‐Collagen I (R26615; ZEN‐BIO), anti‐Runx2 (860139; ZEN‐BIO), anti‐ALP (381009; ZEN‐BIO), anti‐ULK1(381887; ZEN‐BIO), anti‐Phospho‐ULK1 (Ser758) (AF4387; Affinity Biosciences), anti‐mTOR (380411; ZEN‐BIO), anti‐Phospho‐mTOR (Ser2448) (381557; ZEN‐BIO). Secondary antibodies included: Goat anti‐mouse IgG (H&L) (HRP conjugated) (511103; ZEN‐BIO) and Goat anti‐rabbit IgG (H&L) (HRP conjugated) (511203, ZEN‐BIO).

Zhu L., Wang J., Wu Z., Chen S., He Y., Jiang Y., Luo G., Wu Z., Li Y., Xie J., Zou S, & Zhou C. (2023). AFF4 regulates osteogenic potential of human periodontal ligament stem cells via mTOR‐ULK1‐autophagy axis. Cell Proliferation, 57(2), e13546.

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Immunoblot Analysis in 293T Cells

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Human embryonic kidney 293T cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) and tested for mycoplasma contamination periodically by a PCR-based mycoplasma detection kit (Beyotime Biotec).
For immunoblot analysis, the following antibodies were used: Mouse anti-Flag (sc-7392, Santa Cruz Biotechnology), Rabbit anti-HA (H6908, Sigma-Aldrich). Rabbit anti-Myc (16286-1), anti-V5 (14440-1), Mouse anti-GFP (66002-1), Mouse anti-GAPDH (60004-1), Mouse anti-Tubulin (66031-1), goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP were from Proteintech. Rabbit anti-Flag (14793S), anti-HA (3724S), anti-p65 (8284S), anti-IRF3 (11904T) were purchased from Cell Signaling Technology. Rabbit anti-STAT1 (AF0288) and anti-LaminB1 (AF5222) were from Beyotime Biotec. Mouse anti-HA (201113) and anti-β-Actin (200068-8F10) antibodies were purchased from Zen-Bioscience.

Ran X.H., Zhu J.W., Chen Y.Y., Ni R.Z, & Mu D. (2022). Papain-like protease of SARS-CoV-2 inhibits RLR signaling in a deubiquitination-dependent and deubiquitination-independent manner. Frontiers in Immunology, 13, 947272.

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