NaCl (Cat #S5886), CaCl2 (Cat #C8106) and dimethyl sulfoxide (DMSO, #276855-100) were purchased from Sigma. Low-yield tissue-disruptor Mix (Cat #P01-3002) and Cell Implantation Resuspension Medium (#P03-2001) were purchased from BioReperia, and RPMI-1640 culture medium (Cat #LM-R1637/500) was purchased from Biosera. Foetal bovine serum (Cat #97068-085), MgSO4 (Cat #0662), KCl (Cat #26764.232), and phosphate buffered saline (PBS, #E403-500) were purchased from VWR. Gentle MACS™ C-tube (Cat #130096334) was purchased from Miltenyi Biotec. Trypan blue 0.4% solution (Cat #15250061) and Fast-DiI™ oil (#D3899) were purchased from ThermoFisher Scientific. Carboplatin (Cat #S1215) and paclitaxel (Cat #S1150) were purchased from Selleckchem. 1-Phenyl-2-Thiourea (aka PTU, Cat #L06690) was purchased from Alfa Aesar. Penicillin-Streptomycin (#L0022-100) and Trypsin/EDTA (#MS0158100U) were purchased from Biowest.
Foetal bovine serum (fbs)
Manufactured by Avantor
Foetal bovine serum is a cell culture supplement derived from the blood of bovine foetuses. It provides a rich source of proteins, hormones, and other growth factors essential for the proliferation and maintenance of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Trypan blue solution 0.4, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Biowest (1 mentions)
Gentlemacs c tube, by Miltenyi Biotec (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Avantor (1 mentions)
Mgso4, by Avantor (1 mentions)
Cacl2, by Merck Group (1 mentions)
Trypsin edta, by Biowest (1 mentions)
Fast dii oil, by Thermo Fisher Scientific (1 mentions)
Carboplatin, by Selleck Chemicals (1 mentions)
Paclitaxel, by Selleck Chemicals (1 mentions)
1 phenyl 2 thiourea aka ptu, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 culture medium, by Biosera (1 mentions)
Penicillin streptomycin, by Avantor (1 mentions)
B16f10 murine melanoma cells, by American Type Culture Collection (1 mentions)
3 protocols using foetal bovine serum (fbs)
1
Comprehensive Cell Isolation and Culture
2
B16F10 Murine Melanoma Cell Culture
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B16F10 murine melanoma cells were acquired from the American Type Culture Collection (CRL‐6475™; Manassas, VA) and cultured in high‐glucose Dulbecco's Modified Eagle Medium (10‐013‐CV; Corning, Corning, NY) containing L‐glutamine and sodium pyruvate, supplemented with 10% foetal bovine serum (89510‐196; VWR, Radnor, PA) and 1× penicillin/streptomycin (VWR 97063‐708). Modified Jurkat ADCC effector cells expressing hFcγRIIIa and firefly luciferase downstream of an NFAT response element were obtained from Promega (G7102; Madison, WI) and cultured according to the kit protocol. All cells were incubated at 37 °C with 5% CO2.
3
Carboplatin-induced Myotube Atrophy in C2C12 Cells
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C2C12 myoblasts (CRL‐1772, ATCC, Manassas, VA) were cultured subconfluently in Dulbecco's modified Eagle's media (DMEM) (Hyclone, Logan, UT) with 10% foetal bovine serum (VWR, Radner, PA). Differentiation to myotubes was initiated by replacing growth media with Dulbecco's modified Eagle's media supplemented with 2% horse serum (Hyclone) plus 1% foetal bovine serum for 4 days, refreshing media every 48 h. Three‐hundred‐micromolar carboplatin was diluted in differentiation media and applied to cells for 24 or 48 h prior to harvest. Three hundred micromolars was the lowest effective dose to induce myotube atrophy. Another set of myoblasts were differentiated for 6 days before carboplatin treatment as described above to confirm effects in myotubes under extended differentiation. Cells were maintained at 37°C with 5% CO2 in a humidified chamber. Three biological replicates were used for analysis, and all experiments were repeated at least twice to confirm findings.
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