CSBTA group was treated with 0, 0.05, 0.5, 5, 50 μg/ml of CSBTA, and paclitaxel groups were given 0, 1, 10, 100, 500, 1000 nM paclitaxel (A4393, APExBIO, USA) on the 4th day of primary DRG neurons. Five days after administration, a Cell Counting Kit-8 (CCK-8) solution (Dojindo, Japan) was added. After incubation for 4 h, absorbance at 450 nm was measured with a microplate reader.
Paclitaxel
Manufactured by Apexbio
Sourced in United States
Paclitaxel is a natural product that is isolated from the bark of the Pacific yew tree. It is a widely used anticancer agent that has been approved for the treatment of various types of cancer, including ovarian, breast, and lung cancer.
Automatically generated - may contain errors
Lab products found in correlation
Puromycin, by Merck Group (3 mentions)
Docetaxel, by Apexbio (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Paclitaxel, by Solarbio (1 mentions)
Tunicamycin, by Solarbio (1 mentions)
Tunicamycin, by Thermo Fisher Scientific (1 mentions)
Cck 8 assay, by Dojindo Laboratories (1 mentions)
Recombinant human il 1β, by Sangon (1 mentions)
Paclitaxel, by Thermo Fisher Scientific (1 mentions)
Il 1β, by Solarbio (1 mentions)
Polyethylene glycol, by Merck Group (1 mentions)
Sodium polystyrenesulfonate pss, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Doxycycline, by LKT Laboratories (1 mentions)
Doxorubicin, by Thermo Fisher Scientific (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Irinotecan, by Merck Group (1 mentions)
Cisplatin, by Adipogen (1 mentions)
8 protocols using paclitaxel
1
Dose-Dependent Effects of CSBTA and Paclitaxel on DRG Neurons
2
Cell Viability Evaluation by CCK-8 Assay
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The CCK-8 assay (Dojindo Molecular Technologies, Inc.) was performed to test cell viability. Following treatment with 8 nM paclitaxel (APeXBIO Technology LLC), 20 ng/ml recombinant human IL-1β (Sangon Biotech Co., Ltd.), 0.1 µg/ml tunicamycin (Beijing Solarbio Science & Technology Co., Ltd.), paclitaxel + IL-1β, paclitaxel + tunicamycin and blank control, then 2x103 A549 cells were seeded into 96-well plates and incubated at 37˚C with 5% CO2 for 48 h. At 0, 12, 24 and 48 h, 10 µl CCK-8 reagent was added to each well and incubated with the cells for 2 h. Absorbance was measured at 450 nm by a microplate reader (Thermo Fisher Scientific, Inc.).
3
Synthesis and Characterization of PLA-Based Nanocarriers
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Amino-terminated poly-L-lactide (PLA-NH2) with Mn = 2500 and PDI ≤ 1.3 (see Figure 1 I), polyethyleneglycol methyl ether-block-poly-D,L-lactide (PLA–PEG) with polyethylene glycol Mn = 2000 and polylactide Mn = 2000 and PDI < 1.4 (Figure 1 II), from Sigma-Aldrich (St. Louis, MO, USA), were used as received.
The antibiotic paclitaxel (Figure 1 III) from ApexBio (Houston, TX, USA) and the pharmaceutical form of paclitaxel (Taxol®)- paclitaxel-Teva (macrogol glyceryl ricinyl oleate, anhydrous citric acid and absolute ethanol containing composition), from Pharmachemie (Haarlem, The Netherlands) were used as received.
The enzyme trypsin, sodium polystyrenesulfonate (PSS) with Mw = 200,000, sodium acetate and tris-hydroxyaminomethan (Tris) from Sigma-Aldrich (St. Louis, MO, USA) were used as received.
Bidistilled water (DI water) was used in all experiments.
The antibiotic paclitaxel (
The enzyme trypsin, sodium polystyrenesulfonate (PSS) with Mw = 200,000, sodium acetate and tris-hydroxyaminomethan (Tris) from Sigma-Aldrich (St. Louis, MO, USA) were used as received.
Bidistilled water (DI water) was used in all experiments.
4
Compound Solubilization for In Vitro Assays
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STL427944 and its derivative STL001 (Vitas-M Laboratory, Hong Kong), Paclitaxel (APExBIO Technology, USA), 5-FU (LKT Laboratories, USA), Doxorubicin (Thermo Fisher Scientific), and Irinotecan (Millipore Sigma) were dissolved in DMSO (Millipore Sigma). Tamoxifen (Millipore Sigma) was dissolved in ethanol. Cisplatin (AdipoGen Life Sciences, USA) was dissolved in 5% D-glucose solution in sterile water. Doxycycline (LKT Laboratories) and Puromycin (Millipore Sigma) were dissolved in sterile water. Estrogen, β-Estradiol (E2) was provided by Dr. Hisham Mohammed (Oregon Health & Science University) dissolved in ethanol.
5
Comprehensive Panel of Breast Cancer Cell Lines
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MDA-MB-231, MCF7, T47D, SKBR3, BT474, BT-20, MDA-MB-468, Hs578t and MDA-MB-453 and MDA-MB-157 cells purchased from the American Type Culture Collection (Manassas, VA). MFM 223, SUM159PE and SUM185PE cells were kindly provided by Dr. Jennifer A. Pietenpol (Vanderbilt-Ingram Cancer Center). ACK1, AR, EGFR, Her2, Her3, Mcl-1, Bcl-2, Puma, YB-1, FAK, AKT, Anti-phospho-AKT (Ser473), anti-phospho-S6K1 (S79), Anti-phospho-4-EBP1, Anti-phospho-LRP6, Anti-phospho-mTOR, Anti-phospho-YB-1, Anti-phospho-FAK (Y397), anti- ribosomal protein S6, anti-ribosomal protein S6, BCL-xL and anti-cleaved Casp3 antibodies were obtained from Cell Signaling Technology (Beverly, MA). Anti-phospho-ribosomal protein S6 (S235/236), mouse anti-ERK antibody and Anti-phospho-ERK were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Vinculin was purchased from Sigma. Secondary anti-mouse IgG with horseradish peroxidase was from Calbiochem. Secondary anti-rabbit IgG with horseradish peroxidase was from GE Healthcare. Ceritinib and Paclitaxel were purchased from APExBIO. Bicalutamide (97%) purchased from ACROS Organics. The 133 FDA-approved drugs were kindly provided from NCI/DTP Approved Oncology Drugs Plated Set (AODVIII).
6
Cytotoxic Drug Procurement Protocol
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Doxorubicin, paclitaxel, docetaxel and 4-hydroperoxy cyclophosphamide were purchased from ApexBio (Houston, TX, USA). Puromycin was purchased from Sigma-Aldrich (Shanghai, China).
7
Comprehensive Drug Dissolution Protocol
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STL427944 (Vitas-M Laboratory, Hong Kong), actinomycin D (ActD, MilliporeSigma, Burlington, MA, USA), cycloheximide (CHX, MilliporeSigma), bafilomycin A1 (BafA1, AdipoGen Life Sciences, San Diego, CA, USA), bortezomib (APExBIO Technology, Houston, TX, USA), MG132 (Tocris Bioscience, Minneapolis, MN, USA), docetaxel (APExBIO Technology), paclitaxel (APExBIO Technology), and 5-FU (LKT Laboratories, St Paul, MN, USA) were dissolved in DMSO. Doxycycline (LKT Laboratories), chloroquine (CQ, LKT Laboratories), and puromycin (MilliporeSigma) were dissolved in sterile water. Carboplatin (AdipoGen Life Sciences) was dissolved in sterile 5% d -glucose (MilliporeSigma) solution in water. Leptomycin B (LMB, Alfa Aesar, Haverhill, MA, USA) was dissolved in ethanol.
8
Preparation and Storage of Propofol and Paclitaxel
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Propofol was purchased from Sigma-Aldrich (1572503, St. Louis, MO, United States). Propofol injectable emulsion (PIE) was obtained from AstraZeneca Co., United Kingdom. Paclitaxel was from Apexbio (A4393, Houston, TX, United States). All the reagents were dissolved according to the manufacturer’s instructions. Stock solutions of Propofol and Paclitaxel were stored in −20°C, while PIE was stored in 4°C.
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