Anti rabbit alexa fluor 488 af488 secondary antibody

Sorafenib was purchased from Selleck (Houston, TX, USA). Total-FOXO3a (t-FOXO3a; 12829S), phospho-FOXO3a (Ser253; 9466S), total-AKT (t-AKT; 4685S), phospho-AKT (Ser473; 4058S), LC-3B (3868S) primary antibodies for western blot and immunofluorescence were obtained from Cell Signaling (Danvers, MA, USA). The p62 (ab109012) primary antibody for western blot and immunohistochemical (IHC) was purchased from Abcam (Cambridge, MA, USA). The β-actin (66009-1-Ig) primary antibody was purchased from ProteinTech (Rosemont, IL, USA). The HRP-conjugated secondary antibodies were purchased from Beijing ZhongShan Biotechnology Company (Beijing, China). Anti-rabbit Alexa Fluor 488 (AF488) secondary antibody was purchased from Invitrogen (Carlsbad, CA, USA).

Salinomycin, doxorubicin and PKF 118-310 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Total-FOXO3a (t-FOXO3a), phospho-FOXO3a (Thr32; p-FOXO3a), total-AKT (t-AKT), phospho-AKT (Ser473; p-AKT), E-cadherin, Vimentin, β-catenin and TCF4 primary antibodies for western blotting, immunoprecipitation or immunofluorescence were obtained from Cell Signaling (Danvers, MA, USA). GAPDH primary antibodies were obtained from Kangchen Biotechnology (Sichuan, China). The HRP-conjugated secondary antibodies, goat-anti-mouse second antibody and goat-anti-rabbit second antibody, were purchased from Beijing ZhongShan Biotechnology Company (Beijing, China). Propidium iodide (PI) and anti-rabbit Alexa Fluor 488 (AF488) secondary antibody were both purchased from Invitrogen (Carlsbad, CA, USA).