Arf1 antibodies

Human SDF1α was purchased from PeproTech; UK14304, rapamycin, brefeldin A, monensin, nigericin, swainsonine, and Exo2 were from Sigma–Aldrich; pertussis toxin was from List Biological Laboratories; gallein was from Tocris Bioscience; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; secinH3, GCA, nocodazole, ilimaquinone, AMD3100, control siRNA, and siRNA targeting human ARF1, CRISPR–Cas9 control plasmids, and knockout plasmids targeting human ARF1, and antibodies against phospho-ERK1/2 and β-actin were from Santa Cruz Biotechnology; p230 antibodies were from BD Transduction Laboratories; ARF1 antibodies were purchased from Abcam; ERK1/2 antibodies were from Cell Signaling Technology; 12-well inserts and Matrigel matrix were from Corning. All other materials were obtained as described elsewhere (9 (link), 19 (link), 27 (link)).

Exo2, BFA and Secin H3 were obtained from Selleckchem (Houston, TX). Salirasib and β-actin antibody were purchased from Sigma-Aldrich (St Louis, MO). Antibodies that recognize p-AKT, p-ERK1/2, p-STAT3, p-Src, AKT, ERK1/2, STAT3, Src and cleaved (c)-PARP were purchased from Cell Signaling Technology (Beverly, MA). Ki67 and Arf1 antibodies were purchased from Abcam (Cambridge, MA). Cell proliferation was determined by CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, MI) and crystal violet staining. Apoptosis was determined by FITC Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, San Diego, CA). Western blot, Arf1 activation, and Transwell invasion analyses were carried out as described previously [21 (link), 28 (link)–31 (link)].