Plasma was obtained by centrifugation immediately after collection. Blood chemical parameters were measured by using an automatic chemistry analyzer (Mindray, BS220). Blood chemical parameters were as follows: C-reactive protein (CRP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), lactate dehydrogenase (LDH), and triglyceride (TG). Plasma concentrations of IL-6 and soluble IL-6R (sIL-6R) were measured by ELISA kit for human IL-6 (R&D, Cat# DY206-05) and human IL-6R (R&D, Cat# DY227), respectively.
Elisa kit for human il 6
Manufactured by R&D Systems
Sourced in United States
The ELISA kit for human IL-6 is a quantitative sandwich enzyme immunoassay designed for the measurement of human interleukin-6 (IL-6) in cell culture supernatants, serum, and plasma. The kit utilizes a monoclonal antibody specific for human IL-6 that has been pre-coated onto a microplate. The assay procedure involves the addition of standards and samples to the wells, followed by the addition of a biotinylated detection antibody specific for human IL-6. Streptavidin-peroxidase is then added, and a substrate solution is used to elicit a color change that is proportional to the amount of human IL-6 present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elisa kit for human il 6
1
Blood Biomarker Panel for Disease Assessment
2
Quantifying IL-6 and Histone H1.5 in Amniotic Fluid
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ELISA kit for human IL-6 (R&D systems, MN, USA) and human histone H1.5 (MyBiosource, CA, USA) were used to determine IL-6 and H1.5 levels in amniotic fluid. Samples with IL-6 concentration above the top calibration point were diluted to fit the calibration range. Resulting absorbance was read at 450 nm with correction to 540 nm. Data was evaluated in GraphPad Prism 6.
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