Pcmv neo bam p53 wild type

RPMI1640 medium, Opti-modified Eagle’s medium (Opti-MEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Life Technologies, Inc. (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), RNase A, leupeptin, aprotinin, phenylmethylsulfonylfluoride, and Triton X-100 were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). The pCMV-Neo-Bam p53-R175H, pCMV-Neo-Bam p53-R248W, pCMV-Neo-Bam p53-R273H, and pCMV-Neo-Bam p53 wild-type were obtained from Addgene (Cambridge, MA, USA). The antibodies against anti-p53, anti-Rad21, anti-S1PR1, anti-THBS1, and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The protein G PLUS-agarose was also obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

RPMI 1640 medium, foetal bovine serum (FBS), penicillin, and streptomycin were obtained from Life Technologies Inc. (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), RNase A, leupeptin, aprotinin, phenylmethylsulfonylfluoride, selective FAK inhibitor 14 (1,2,4,5-benzenetetramine tetrahydrochloride), and Triton X-100 were purchased from Sigma–Aldrich Co. (St Louis, MO, USA). The pCMV-Neo-Bam p53 R248W, pCMV-Neo-Bam p53 R273H, and pCMV-Neo-Bam p53 wild-type were obtained from Addgene (Cambridge, MA). CellTracker^TM was obtained from Invitrogen (Grand Island, NY, USA). The antibodies anti-p53 (DO-1, sc-126), anti-Akt, anti-focal adhesion kinase (FAK), integrin β4, and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies anti-phospho-Akt and anti-phospho-FAK were purchased from Cell Signalling Technology (Beverly, MA, USA). Function-blocking antibody against the human integrin β4 was obtained from EMD Millipore (Billerica, MA, USA)53 (link). The PI3K/Akt inhibitor LY294002 and wortmannin were obtained from Calbiochem (San Diego, CA, USA).