A TriM Scope II multi photon system from LaVision BioTec was used for SHG imaging to visualise anisotropic structures, such as collagen fibres. The microscope setup is a single beam instrument with an upright Olympus BX51 WI microscope stand equipped with high sensitive non-descanned detectors close to the objective lens. The TriM Scope II is fitted with a Coherent Scientific Chameleon Ultra II Ti:Sapphire laser (tuning range 680-1080 nm) and a Coherent Chameleon Compact OPO (automated wavelength extension from 1000 nm to 1600 nm). A 20x IR objective lens (Olympus XLUMPlanFl 20x/1.0W) with a working distance of 2.0 mm was used. SHG signals were detected using TiSa light at 850 nm, a 420/40 band pass filter and a blue-sensitive photomultiplier (Hamamatsu H67080-01). 3D-images were acquired and processed with LaVision BioTec ImSpector Software under Microsoft Windows. Overview images were reconstructed from a sequence of tiled 3D images with Image J.
Xlumplanfl 20x 1.0w
Manufactured by Olympus
The XLUMPlanFl 20x/1.0W is a waterimmersion objective lens designed for microscopy applications. It has a magnification of 20x and a numerical aperture of 1.0. The lens is optimized for use with water-based samples.
Automatically generated - may contain errors
3 protocols using xlumplanfl 20x 1.0w
1
Multi-Photon Imaging of Collagen Fibers
2
Multi-Photon Imaging of Collagen Fibers
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A TriM Scope II multi photon system from LaVision BioTec was used for SHG imaging to visualise anisotropic structures, such as collagen fibres. The microscope setup is a single beam instrument with an upright Olympus BX51 WI microscope stand equipped with high sensitive non-descanned detectors close to the objective lens. The TriM Scope II is fitted with a Coherent Scientific Chameleon Ultra II Ti:Sapphire laser (tuning range 680-1080 nm) and a Coherent Chameleon Compact OPO (automated wavelength extension from 1000 nm to 1600 nm). A 20x IR objective lens (Olympus XLUMPlanFl 20x/1.0W) with a working distance of 2.0 mm was used. SHG signals were detected using TiSa light at 850 nm, a 420/40 band pass filter and a blue-sensitive photomultiplier (Hamamatsu H67080-01). 3D-images were acquired and processed with LaVision BioTec ImSpector Software under Microsoft Windows. Overview images were reconstructed from a sequence of tiled 3D images with Image J.
3
Visualizing Skeletal Muscle Fiber Organization
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A TriM Scope II multi photon system from LaVision BioTec was used to visualize skeletal muscle fiber organization inside organoids and distinct sarcomeres. The microscope setup is a single beam instrument with an upright Olympus BX51 WI microscope stand equipped with highly sensitive nondescanned detectors close to the objective lens. The TriM Scope II is fitted with a Coherent Scientific Chameleon Ultra II Ti:Sapphire laser (tuning range 680-1080 nm) and a Coherent Chameleon Compact OPO (automated wavelength extension from 1000 nm to 1600 nm). A 20x IR objective lens (Olympus XLUMPlanFl 20x/1.0W) with a working distance of 2.0 mm was used.
Muscle fiber SHG signals were detected in forward direction using TiSa light at 850 nm, a 420/40 band pass filter and a blue-sensitive photomultiplier (Hamamatsu H67080-01). 3D-images were acquired and processed with LaVision BioTec ImSpector Software.
Muscle fiber SHG signals were detected in forward direction using TiSa light at 850 nm, a 420/40 band pass filter and a blue-sensitive photomultiplier (Hamamatsu H67080-01). 3D-images were acquired and processed with LaVision BioTec ImSpector Software.
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