Anti mouse cd16 32 antibody 2.4g2

Manufactured by Cytek Biosciences

Sourced in United States

The Anti-mouse CD16/32 antibody (2.4G2) is a laboratory reagent used for the detection and analysis of mouse CD16 and CD32 proteins. It is a rat monoclonal antibody that specifically binds to these cell surface receptors, which are involved in immune cell function. The antibody can be used in various applications such as flow cytometry, immunohistochemistry, and Western blotting to identify and characterize cells expressing CD16 and CD32.

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Lab products found in correlation

Zombie uv dead cell staining kit, by Cytek Biosciences (2 mentions) Unconjugated mouse and rat igg antibodies, by Jackson ImmunoResearch (2 mentions)

Close spelling variants of this product

Anti-CD16/32 (2.4G2, Mouse anti- CD16/32 antibody Anti-mouse CD16/32 Anti-CD16/32 CD16/32

2 protocols using anti mouse cd16 32 antibody 2.4g2

Multicolor Flow Cytometry Antibodies

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Monoclonal antibodies against the following mouse antigens were used in this study: CD3ε (145.2C11), CD4 (RM4-5), CD8α (53–6.7), CD11b (M1/70), CD11c (N418), CD45 (30-F11), CD45R/B220 (RA3-6B2), CD90.2 (30-H12), CD127 (A7R34), CD170/Siglec-F (E50-2440), F4/80 (BM8), IL-10 (JESS-16E3), Ly6C (HK1.4), Ly6G (1A8). Antibodies were purchased from BD Biosciences (San Diego, USA), BioLegend (San Diego, USA), or ThermoFisher Scientific (Carlsbad, USA). Antibodies were conjugated to Pacific Blue, Brilliant Violet 421, V500, Brilliant Violet 510, Brilliant Violet 570, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 785, Brilliant Violet 786, FITC, Alexa Fluor 488, PerCP-Cy5.5, PerCP-eFluor 710, PE, PE-CF594, PE-Cy7, APC, Alexa Fluor 647, Alexa Fluor 700, APC-Cy7, or APC-Fire750. Details on the antibody used in this study are given in the S1 Table. Anti-mouse CD16/32 antibody (2.4G2) and Zombie UV Dead Cell Staining Kit were obtained from Tonbo Biosciences (San Diego, USA) and from BioLegend, respectively. Unconjugated mouse and rat IgG antibodies were purchased from Jackson ImmunoResearch (West Grove, USA).

Özkan M., Eskiocak Y.C, & Wingender G. (2021). The IL-10GFP (VeRT-X) mouse strain is not suitable for the detection of IL-10 production by granulocytes during lung inflammation. PLoS ONE, 16(5), e0247895.

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Comprehensive Mouse Immune Cell Profiling

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Monoclonal antibodies against the following mouse antigens were used in this study: CD3 (145.2C11), CD4 (RM4-5), CD8 (53-6.7), CD11b (M1/70), CD11c (N418), CD45 (30-F11), CD45R/B220 (RA3-6B2), CD90.2 (30-H12), CD127 (A7R34), CD170/Siglec-F (E50-2440), F4/80 (BM8), IL-10 (JESS-16E3), Ly6C (HK1.4), Ly6G (1A8). Antibodies were purchased from BD Biosciences (San Diego, USA), BioLegend (San Diego, USA), eBioscience (San Diego, USA) or ThermoFisher Scientific (Carlsbad, USA). Antibodies Fluor 700, APC-Cy7, APC-eFluor 780 or APC-Fire750. Anti-mouse CD16/32 antibody (2.4G2) and Zombie UV Dead Cell Staining Kit were obtained from Tonbo Biosciences (San Diego, USA) and from BioLegend, respectively. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove, USA).

Özkan M., Eskiocak Y.C., & Wingender G. (2021). The IL-10^GFP(VeRT-X) mouse strain is not suitable for the detection of IL-10 production by granulocytes during lung inflammation.

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