Optisol gs medium

The donor corneas were received from Lions Eye Bank at Albany and Rochester, New York. Corneas were preserved upon recovery in Optisol-GS medium (Bausch and Lomb, Rochester) inside a storage container for corneas and were delivered to the University of Rochester inside a polystyrene box filled with packs of ice. The donor age ranged from 32 to 66 years. Time from death to tissue preservation was about 5 to 22 h. Donor tissue details are summarized in Table 1. Prior to imaging, corneas were stored at room temperature for 1 h to improve the image quality.³⁷ (link) As a reference, a conventional noncontact specular microscope, Kerato Analyzer (EKA-98, Konan Medical Inc., Japan) was first employed to image the central location of the corneal endothelium through the storage container. A USAF calibration target (R1L1S1P, THORLABS) was used to convert the number of pixels into the physical dimension of endothelial cells area. Corneas were then removed from the storage container and mounted onto a corneal artificial chamber (Moria, Inc., France) equipped with a microfluidic system that was used to control the physiological pressure inside the anterior chamber. The latter was perfused using Optisol to avoid air bubbles inside the anterior chamber.