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Anti hexokinase

Manufactured by US Biological
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Anti-Hexokinase is a laboratory reagent used for the inhibition of hexokinase, an enzyme involved in the first step of glycolysis. It can be used to study the effects of hexokinase inhibition on cellular metabolism and signaling pathways.

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Lab products found in correlation

Anti ded1p, by US Biological (2 mentions) Anti gapdh mab374, by Merck Group (1 mentions) Anti ubiquitin, by Merck Group (1 mentions) Hrp conjugated anti rabbit igg, by Merck Group (1 mentions) Anti ha sc 7392, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated anti mouse igg m8770, by Merck Group (1 mentions) Anti myc mms 150r, by Fortrea (1 mentions)

3 protocols using anti hexokinase

1

Yeast Ded1p Western Blot Analysis

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Lysates from yeast strain A14201 at vegetative phase and stage 11 (ndt80 release timecourse) were prepared as described.15 (link) After loading equal amounts of protein on a 10% NEXT gel, denaturing gel electrophoresisand transfer to a PVDF membrane, Western blotting was performedwith anti-Ded1p (Rabbit; 1:5000) and anti-Hexokinase(Rabbit;1:10,000; US Biological) antibodies. Chemiluminescencewas quantified by Imagequant software. Hexokinaseserved as a loading control for normalization.
Guenther U.P., Weinberg D.E., Zubradt M.M., Tedeschi F.A., Stawicki B.N., Zagore L.L., Brar G.A., Licatalosi D.D., Bartel D.P., Weissman J.S, & Jankowsky E. (2018). The helicase Ded1p controls use of near-cognate translation initiation codons in 5′UTRs. Nature, 559(7712), 130-134.
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2

Immunoblotting Antibody Reagents and Dilutions

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Anti-Rpl1 and anti-Rpl3 were generous gifts from Jonathan Warner. Anti-FLAG (F1804; RRID:AB_262044; 1:10,000 dilution) was from Sigma (St. Louis, MO), anti-Hexokinase (H2035-02; 1:10,000 dilution) was from USBiological (Salem, MA), anti-HUWE1 (A300-486A; RRID: AB_2615536; 1:1,000 dilution) was from Bethyl laboratories (Montgomery, TX), anti-HA (SC-7392; RRID:AB_627809; 1:5,000 dilution) was from Santa Cruz (Dallas, TX), anti-myc (MMS-150R; RRID: AB_291325; 1:5,000 dilution) was from Covance (San Diego, CA), anti-Ubiquitin (05–944; RRID: AB_441944; 1:5,000 dilution) and anti-GAPDH (MAB374; RRID: AB_2107445; 1:5,000 dilution) were from EMD Millipore, and anti-His6 (200-332-382; RRID: AB_10704645; 1:5,000 dilution) was from Rockland (Limerick, PA). For secondary antibody, HRP-conjugated anti-rabbit IgG (A6154; RRID: AB_258284; 1:10,000 dilution) and HRP-conjugated anti-mouse IgG (M8770; RRID: AB_260711; 1:10,000 dilution) were from Sigma, IR680RD conjugated anti-rabbit (926–68071; RRID: AB_10956166; 1:10,000 dilution) and IR800CW conjugated anti-mouse (926–32210; RRID: AB_621842; 1:10,000 dilution) were from LI-COR Biosciences (Lincoln, NE).
Sung M.K., Porras-Yakushi T.R., Reitsma J.M., Huber F.M., Sweredoski M.J., Hoelz A., Hess S, & Deshaies R.J. (2016). A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. eLife, 5, e19105.
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3

Yeast Ded1p Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates from yeast strain A14201 at vegetative phase and stage 11 (ndt80 release timecourse) were prepared as described.15 (link) After loading equal amounts of protein on a 10% NEXT gel, denaturing gel electrophoresisand transfer to a PVDF membrane, Western blotting was performedwith anti-Ded1p (Rabbit; 1:5000) and anti-Hexokinase(Rabbit;1:10,000; US Biological) antibodies. Chemiluminescencewas quantified by Imagequant software. Hexokinaseserved as a loading control for normalization.
Guenther U.P., Weinberg D.E., Zubradt M.M., Tedeschi F.A., Stawicki B.N., Zagore L.L., Brar G.A., Licatalosi D.D., Bartel D.P., Weissman J.S, & Jankowsky E. (2018). The helicase Ded1p controls use of near-cognate translation initiation codons in 5′UTRs. Nature, 559(7712), 130-134.
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