Perforin b d48 pe

Manufactured by Cell Sciences

Perforin (B-D48: PE) is a protein that is involved in the cytotoxic function of natural killer cells and cytotoxic T lymphocytes. It plays a crucial role in the immune system's ability to eliminate infected or transformed cells. The B-D48: PE label refers to the specific clone and conjugation used for this product.

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Lab products found in correlation

Cd107a, by BD (3 mentions) Staphylococcal enterotoxin b, by Merck Group (3 mentions) Cd49d l25, by BD (2 mentions) Pepmix hiv nef ultra, by JPT Peptide Technologies (2 mentions) Cd4 t4d11 ecd, by Beckman Coulter (2 mentions) T bet 4b10 ax647 unlabeled cd28 l293, by BD (2 mentions) Eomesodermin wd1928 pe efluor610, by Thermo Fisher Scientific (2 mentions) Unlabeled functional grade cd3 okt3, by Thermo Fisher Scientific (2 mentions) Unlabeled pan anti tgf β cat no t9429, by Merck Group (2 mentions) Rabbit igg cat no i5006, by Merck Group (2 mentions) T bet, by BioLegend (2 mentions) Mip 1β, by BD (1 mentions) Granzyme b, by BD (1 mentions) Tnf α, by BD (1 mentions) Ifn γ, by BD (1 mentions)

3 protocols using perforin b d48 pe

Multiparameter Flow Cytometry Panel

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The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (BG11: V450) from BD Biosciences (San Jose, CA); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), Granzyme A (CB9: Ax647), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). T-bet (4B10: Ax647); unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and unlabeled functional grade CD3 (OKT3) were from eBiosciences (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). Unlabeled pan anti-TGF-β (Cat No. T9429) and rabbit IgG (Cat No. I5006) were from Sigma Aldrich. The HIV Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). HIV Nef peptide pool (PepMix™ HIV NEF Ultra, JPT Peptide Technologies, Berlin, Germany) consisted of 150 15-mers with an 11 amino acid overlap. Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.

Kiniry B.E., Ganesh A., Critchfield J.W., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2016). Predominance of Weakly Cytotoxic, T-betLowEomesNeg CD8+ T-cells in Human Gastrointestinal Mucosa: Implications for HIV Infection. Mucosal immunology, 10(4), 1008-1020.

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Multiparameter Flow Cytometry Panel

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Comprehensive Flow Cytometry Antibody Panel

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The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (GB11: V450, PE-CF594), MIP-1β (D21-1351: APC-H7, PerCP-Cy5.5), IFNγ (B27: PE-Cy7), TNFα (Mab11: Ax700), CD69 (L78: PE) from BD Biosciences (San Jose, CA); Granzyme K (GM6C3: FITC) from Santa Cruz Biotechnology, Inc. (Dallas, Tx); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), CD103 (Ber-ACT8: Ax488), Granzyme A (CB9: Ax647, PacBlue), and CD3 (UCHT-1: Ax700) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). S1PR1 (SW4GYPP: eFluor660) was purchased from eBioscience (San Diego, CA); Unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). This perforin antibody detects de novo as well as pre-formed perforin and is suitable for monitoring perforin expression following TCR stimulation (21 (link)). The HIV-1 Gag peptide pool (Consensus Clade B) consisted of 123 15-mers overlapping by 11 residues (JPT Innovative Peptide Solutions, Berlin, Germany). Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.

Kiniry B.E., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2018). Differential Expression of CD8+ T Cell Cytotoxic Effector Molecules in Blood and Gastrointestinal Mucosa in HIV-1 Infection. Journal of immunology (Baltimore, Md. : 1950), 200(5), 1876-1888.

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