Goat anti mouse igg h l

Manufactured by Bio-Rad

Sourced in United States

Goat anti-mouse IgG (H+L) is a secondary antibody that binds to the heavy and light chains of mouse immunoglobulin G (IgG). This product is commonly used in various immunoassay techniques, such as Western blotting and ELISA, to detect and quantify the presence of mouse antibodies in samples.

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Lab products found in correlation

Goat anti rabbit igg h l, by Bio-Rad (7 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (3 mentions) Anti human nfat1 1 nfat 1 antibody, by BD (3 mentions) Anti human β actin ac 15 antibody, by Merck Group (3 mentions) Penicillin streptomycin, by Corning (2 mentions) Atr n 19, by Santa Cruz Biotechnology (2 mentions) Human igg fc fragment, by Jackson ImmunoResearch (1 mentions) 2 iminothiolane hydrochloride, by Merck Group (1 mentions) P p53 ser15, by Cell Signaling Technology (1 mentions) Affinipure donkey anti goat igg, by Jackson ImmunoResearch (1 mentions) Amersham ecl, by Cytiva (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Vimentin, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Anti human rybp, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Polyethylenimine (pei), by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Goat anti-mouse IgG (H+L)-HRP conjugate (90 citations) Goat anti-mouse IgG (H+L)-HRP (18 citations) HRP-conjugated goat anti-mouse IgG (H+L) (9 citations) HRP-conjugated goat anti-mouse IgG (H+L) antibody (5 citations) Goat anti-mouse IgG (H + L) horseradish peroxidase conjugate (4 citations) Goat anti-mouse IgG (H+L) HRP-conjugated secondary antibodies (3 citations) Goat anti-mouse and anti-rabbit IgG (H+L) horseradish peroxidase secondary antibodies (3 citations) Goat anti-mouse IgG (H + L)-horseradish peroxidase (HRP) conjugate (3 citations) Goat-anti-mouse IgG (H+L)-HRP conjugate 1706516 (2 citations) Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (2 citations)

9 protocols using goat anti mouse igg h l

Characterization of SP141 and FcRn Binding

Cited 2 times

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The small molecule MDM2 inhibitor SP141 was synthesized and characterized as described in our previous studies [12 (link), 14 (link)]. The Mal-PEG-PCL copolymers (6 KDa) were purchased from Advanced Polymer Materials (Montreal, Canada). Human IgG Fc fragments were sourced from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). 2-Iminothiolane hydrochloride was obtained from Sigma (St Louis, MO, USA). DilC₁₈(5) oil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate) was purchased from Thermo Scientific (Rockford, IL, USA). Fetal bovine serum was obtained from Atlanta Biologicals (Lawrenceville, GA, USA). The penicillin/streptomycin was bought from Corning (Manassas, VA, USA). The antibodies against human p53 (DO-1; 1:2000) and FcRn (H-4; 1:1000) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against human MDM2 (Ab-2; 1:500) and p21 (Ab-1; 1:1000) were bought from EMD Chemicals (Gibbstown, NJ, USA). The goat antimouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were obtained from Bio-Rad (Hercules, CA, USA).

Qin J.J., Wang W., Sarkar S, & Zhang R. (2016). Oral delivery of anti-MDM2 inhibitor SP141-loaded FcRn-targeted nanoparticles to treat breast cancer and metastasis. Journal of controlled release : official journal of the Controlled Release Society, 237, 101-114.

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Molecular Mechanisms of Cellular Regulation

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All chemicals and solvents used in the present study were of highest grade available. InuA with a purity higher than 95% was prepared as reported previously [34 (link)]. The antibodies against human MDM2 (Ab-2), p21 (Ab-1), and p-H2AX (Ser139) were bought from EMD Chemicals (Gibbstown, NJ, USA). The antibodies against human p53 (DO-1), Cdk2 (M2), Cdk4 (H-22), Cdk6 (C-21), cyclin D1 (DCS-6), cyclin E (HE12), c-Myc (0.N.222), Bax (N-20), Bcl-2 (100), PARP (H-250), Chk1 (G-4), Chk2 (B-4), and ATR (N-19) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against human p-Chk1 (Ser317), p-Chk2 (Thr68), and p-p53 (Ser15) were obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-human NFAT1 (1/NFAT-1) antibody was sourced from BD Biosciences (San Jose, CA, USA) and the anti-human β-actin (AC-15) antibody was purchased from Sigma (St. Louis, MO, USA). The goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were bought from Bio-Rad (Hercules, CA, USA). The NFAT1 and MDM2 siRNA pool and control siRNA pool were obtained from Thermo Scientific (Rockford, IL, USA).

Qin J.J., Wang W., Sarkar S., Voruganti S., Agarwal R, & Zhang R. (2016). Inulanolide A as a new dual inhibitor of NFAT1-MDM2 pathway for breast cancer therapy. Oncotarget, 7(22), 32566-32578.

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Immunoprecipitation and Western Blotting Protocols for Detecting DCTN1, TDP-43, and β-Tubulin

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Antibodies against DCTN1 (goat polyclonal; Abcam, Cambridge, UK; ab11806), TDP-43 (against the C-terminal region; rabbit polyclonal; Proteintech; #12892-1-AP), and β-tubulin (mouse monoclonal (mAb); Sigma-Aldrich, St. Louis, MO, USA; TUB2.1) were used for immunoprecipitation and/or Western blotting. Antibodies against GFP (polyclonal; MBL; #598), RFP (mouse monoclonal cocktail; MBL; M208-3), c-Myc (mouse monoclonal used for immunoprecipitation; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 9E10), Myc-tag (rabbit polyclonal used for Western blotting; MBL; #562), and horseradish peroxidase (HRP)-conjugated anti-RFP antibody (MBL; PM005-7) were also used for the detection of tagged DCTN1 and TDP-43 proteins.
HRP-conjugated secondary antibodies used for Western blotting in this study were: goat antimouse IgG (H + L; Bio-Rad, Hercules, CA, USA; #1706516), AffiniPure donkey antigoat IgG (Jackson ImmunoResearch, West Grove, PA, USA; #705-035-147), Amersham ECL donkey antirabbit IgG (Cytiva; NA934), and mouse antirabbit IgG (conformation-specific) mAb (Cell Signaling Technology, Danvers, MA, USA; L27A9; #5127).

Deshimaru M., Kinoshita-Kawada M., Kubota K., Watanabe T., Tanaka Y., Hirano S., Ishidate F., Hiramoto M., Ishikawa M., Uehara Y., Okano H., Hirose S., Fujioka S., Iwasaki K., Yuasa-Kawada J., Mishima T, & Tsuboi Y. (2021). DCTN1 Binds to TDP-43 and Regulates TDP-43 Aggregation. International Journal of Molecular Sciences, 22(8), 3985.

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Molecular Markers for Cancer Progression

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All chemicals and solvents were of the highest analytical grade available. Cell culture supplies and media, fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin-streptomycin were obtained from Invitrogen (Carlsbad, CA). The anti-human p53, Bax, and poly (ADP-ribose) polymerase (PARP) antibodies were from Santa Cruz Biotechnology Inc. (Dallas, TX). The anti-human E-cadherin antibody was from BD Biosciences (San Jose, CA). The anti-human RYBP, vimentin, and β-actin antibodies were from Sigma (St. Louis, MO). Goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) were obtained from Bio-Rad (Hercules, CA). The preparation of the Myc-RYBP expression vector was described previously. The RYBP siRNA pool and control siRNA pool were obtained from Dharmacon.

Wang W., Cheng J., Qin J.J., Voruganti S., Nag S., Fan J., Gao Q, & Zhang R. (2014). RYBP expression is associated with better survival of patients with hepatocellular carcinoma (HCC) and responsiveness to chemotherapy of HCC cells in vitro and in vivo. Oncotarget, 5(22), 11604-11619.

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Synthesis and Characterization of Anticancer Nanoparticles

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Poly(methacrylic acid) (PMAA, average M_w 21800 g mol⁻¹), poly(N-vinylpyrrolidone) (PVPON, average M_w 58000 g mol⁻¹), poly(ethylenimine) (PEI, average M_w 25000 g mol⁻¹), were purchased from Sigma-Aldrich. 1-Ethyl-3-(3-(dimethylamino)propyl)-carbodiimide hydrochloride (EDC) was obtained from Chem-Impex International. Monobasic sodium phosphate, hydrochloric acid, sodium hydroxide, L-glutathione reduced (GSH), ethylenediaminetetraacetic acid (EDTA) and methanol were from Fisher Scientific and used as received. Ultrapure deionized water with a resistivity of 18 MΩ cm was used in all experiments (Evoqua). The anti-human p53 (DO-1) antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The anti-human MDM2 (Ab-2) and p21 (Ab-1) antibodies were from EMD Chemicals, Inc. (Gibbstown, NJ, USA). The anti-human β-actin (AC-15) antibody was from Sigma (St. Louis, MO, USA). The goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) were obtained from Bio-Rad (Hercules, CA, USA). Fetal bovine serum was obtained from Atlanta Biologicals (Lawrenceville, GA, USA). Penicillin/streptomycin was from Corning (Manassas, VA, USA). Porous cubic Mn₂O₃ microparticles (1.5–2 μm) were synthesized as described previously [37 ]. Anti-cancer drug BA-TPQ was synthesized as described previously [47 ] (see Supplementary Material for details).

Xue B., Wang W., Qin J.J., Nijampatnam B., Murugesan S., Kozlovskaya V., Zhang R., Velu S.E, & Kharlampieva E. (2017). Highly efficient delivery of potent anticancer iminoquinone derivative by multilayer hydrogel cubes. Acta biomaterialia, 58, 386-398.

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Investigating NFAT1 Regulation of MDM2

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JapA was obtained as previously reported [13 (link)], with the purity being > 95% as confirmed by IR, ESI-MS, NMR, and HPLC/MSⁿ [16 (link)–17 (link)]. All chemicals and solvents were of the highest analytical grade available. The anti-human NFAT1 (1/NFAT-1) antibody was from BD Biosciences (San Jose, CA). The anti-human COX-2 (C-20) and c-Myc (0.N.222) antibodies were from Santa Cruz Biotechnology Inc. (Dallas, TX). The anti-human MDM2 (Ab-2) antibody was from Calbiochem (Billerica, MA). The anti-human ubiquitin (6C1) and β-actin (AC-15) antibodies were from Sigma (St. Louis, MO). Goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) were obtained from Bio-Rad (Hercules, CA). The human full-length MDM2 P2 promoter reporters were kind gifts from Dr. J.P. Blaydes (Southampton General Hospital, UK). Vectors expressing HA-NFAT1, CA-NFAT1, and DN-NFAT were kindly provided by Dr. C.W. Chow (Yeshiva University). NFAT1 siRNA or control siRNA were from Thermo Scientific (Rockford, IL). Both plasmids and siRNAs were transfected into cells using the same protocols as we reported previously [57 (link)].

Qin J.J., Wang W., Voruganti S., Wang H., Zhang W.D, & Zhang R. (2015). Inhibiting NFAT1 for breast cancer therapy: New insights into the mechanism of action of MDM2 inhibitor JapA. Oncotarget, 6(32), 33106-33119.

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Immunoblot and Immunofluorescence Protocols

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For immunoblot analysis, the following primary antibodies were used: MT1-MMP (1:1000, AB6004, Millipore); MT1-MMP (1:1000, AB51074, Abcam); Phospho-ERK1/2 (1:2000, D13.14.4E), ERK1/2 (1:2000, 137 F5) (Cell Signaling Technology); TIMP-2 (1:1000, 3A4), β-Actin (1:1000, C4), and phospho-histone-3 (PH3) (1:5000, C1513) (Santa Cruz). Goat anti-mouse IgG (H + L) (Bio-Rad) and goat anti-rabbit IgG (H + L) (Thermo Fisher) HRP conjugates were used as secondary antibodies (1:10000). For immunofluorescence analysis we used MT1-MMP antibody AB6004 (1:200), and anti-rabbit-IgG-Alexa488 or Alexa594 (Thermo Fisher) as secondary antibodies (1:400).

Cepeda M.A., Pelling J.J., Evered C.L., Williams K.C., Freedman Z., Stan I., Willson J.A., Leong H.S, & Damjanovski S. (2016). Less is more: low expression of MT1-MMP is optimal to promote migration and tumourigenesis of breast cancer cells. Molecular Cancer, 15, 65.

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Terphenyllin Synthesis and Antibody Validation

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The test compound terphenyllin (Figure 1A) was prepared in Dr. Weiyi Wang’s laboratory (Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China), and the structure was confirmed by NMR, MS, UV, and IR spectroscopy. The purity of terphenyllin was greater than 98%. All chemicals and solvents used were of the highest analytical grade available. The anti-rabbit Bax (D2E11), Bad (D24A9), Puma (D30C10), Bim (C34C5), Bcl-2 (D55G8), phos-Bcl-2 (p-Ser70) (5H2), Bcl-xl (54H6), caspase7 (D2Q3L), PARP (9542), and GAPDH (D16H11) antibodies were obtained from Cell Signaling Technology (Boston, USA). The goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were obtained from Bio-Rad (Hercules, CA, USA).

Zhang J., Wang W., Zhou Y., Yang J., Xu J., Xu Z., Xu B., Yan L., Cheng X.D., Li M, & Qin J.J. (2020). Terphenyllin Suppresses Orthotopic Pancreatic Tumor Growth and Prevents Metastasis in Mice. Frontiers in Pharmacology, 11, 457.

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Reagents and Antibodies for Protein Analysis

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LinA was prepared as described in our previous study^{[48 ]}. All chemicals and solvents used in the present study were of highest grade available. Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA, USA). The penicillin/streptomycin preparation was bought from Corning (Manassas, VA, USA). The anti-human NFAT1 (1/NFAT-1) antibody was purchased from BD Biosciences (San Jose, CA, USA). The antibodies against human MDM2 (Ab-2), p21 (Ab-1), and p-H2AX (Ser139) were sourced from EMD Chemicals (Gibbstown, NJ, USA). The antibodies against human p53 (DO-1), Cdk2 (M2), Cdk4 (H-22), Cdk6 (C-21), cyclin D1 (DCS-6), cyclin E (HE12), c-Myc (0.N.222), Bax (N-20), Bcl-2 (100), PARP (H-250), Chk1 (G-4), Chk2 (B-4), and ATR (N-19) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against human p-Chk1 (Ser317), p-Chk2 (Thr68), and p-p53 (Ser15 (link)) were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-human β-actin (AC-15) antibody was bought from Sigma (St. Louis, MO, USA) and the goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were obtained from Bio-Rad (Hercules, CA, USA).

Qin J.J., Sarkar S., Voruganti S., Agarwal R., Wang W, & Zhang R. (2016). Identification of lineariifolianoid A as a novel dual NFAT1 and MDM2 inhibitor for human cancer therapy. Journal of Biomedical Research, 30(4), 322-333.

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