Total RNA was extracted from Col-0 plants as described above. Samples were then sent to Edinburgh Genomics for QC check and sequencing. Sample quality was checked using Qubit with the broad range RNA kit (Thermo Fisher Scientific) and Tapestation 4200 with the RNA Screentape for eukaryotic RNA analysis (Agilent). Libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) and then validated. Samples were pooled to create 14 multiplexed DNA libraries, which were paired-end (PE) sequenced on an Illumina HiSeq 4000 platform (Machine name K00166, Run number 346, flowcell AHT2HKBBXX, lanes 5 and 6). On average, 23.9 million 150 nt PE reads were obtained for each sample (Supplemental Table S8 ).
Rna screentape for eukaryotic rna analysis
Manufactured by Agilent Technologies
The RNA ScreenTape is a lab equipment product from Agilent Technologies designed for eukaryotic RNA analysis. It provides a rapid and automated method for the analysis of RNA samples.
Automatically generated - may contain errors
Lab products found in correlation
Qubit with the broad range rna kit, by Thermo Fisher Scientific (2 mentions)
Truseq stranded mrna kit, by Illumina (2 mentions)
Hiseq 4000 platform, by Illumina (2 mentions)
Tapestation 4200, by Agilent Technologies (1 mentions)
Rnalater, by Thermo Fisher Scientific (1 mentions)
Mz16f, by Leica (1 mentions)
Rneasy plant mini kit, by Qiagen (1 mentions)
2 protocols using rna screentape for eukaryotic rna analysis
1
Comprehensive RNA-seq Analysis Pipeline
2
Transcriptome analysis of plant leaves
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RNA extraction and cDNA library preparation and high throughput sequencing Whole plants were harvested in RNAlater TM (ThermoFisher Scientific). Once all samples were collected, leaf 3 primordias and leaf 3 blades were dissected with a scalpel, in a Petri dish filled with RNA later solution, under a Leica MZ 16 F dissecting microscope. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) with on-column DNase digestion. Samples were then sent to Edinburgh Genomics for QC check and sequencing. Briefly, quality check of the samples was performed using Qubit with the broad range RNA kit (Thermo Fisher Scientific) and Tapestation 4200 with the RNA Screentape for eukaryotic RNA analysis (Agilent). Libraries were prepared using the TruSeq Stranded mRNA kit (Illumina), and then validated. Samples were pooled to create 14 multiplexed DNA libraries, which were paired-end sequenced on an Illumina HiSeq 4000 platform.
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